Selenium-enriched fermented soybean meal, preparation method and application

A fermented soybean meal and selenium-enriched technology, applied in the direction of bacteria used in food preparation, application, food science, etc., can solve the problems of limiting the nutritional value and retention of soybean meal, and achieve the effects of overcoming pollution and damage, low cost, and convenient material acquisition

Active Publication Date: 2017-07-21
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the related research on fermented soybean meal stays on improving the protein quality of soybean meal, and there are few reports on the research on selenium-enriched fermented soybean meal, which also limits the further improvement of the nutritional value of soybean meal

Method used

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  • Selenium-enriched fermented soybean meal, preparation method and application
  • Selenium-enriched fermented soybean meal, preparation method and application
  • Selenium-enriched fermented soybean meal, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The strain composition of the fermented soybean meal compound probiotic bacterial liquid of the present embodiment is as follows:

[0040]

[0041] The preparation method of the selenium-enriched fermented soybean meal of the present embodiment is as follows:

[0042] (1) Breeding of selenium-enriched strains

[0043] C.utilis was cultured on the YEPD culture plate for 48-72 hours, picked a single colony into a 250ml Erlenmeyer flask containing 100ml YEPD liquid medium, 180-200rpm, oscillating culture for 48-72 hours, and the bacterial liquid was centrifuged for 3-72 hours. 4 times to make a single cell suspension, so that the cell concentration was 10 8 ~10 9 individual / mL. Take 0.1 mL of the bacterial suspension and evenly spread it on a sterile petri dish, and place it in an ultra-clean workbench to air dry to form a bacterial film. in N + Ion implantation is carried out in the implanter with an energy of 14-18KeV. Six gradient doses were made for each strain,...

Embodiment 2

[0062] The strain composition of the fermented soybean meal compound probiotic bacterial liquid of the present embodiment is as follows:

[0063]

[0064]

[0065] The preparation method of the selenium-enriched fermented soybean meal of the present embodiment is as follows:

[0066] (1) Breeding of selenium-enriched strains

[0067] The specific implementation is the same as in Example 1.

[0068] (2) Preparation of seed culture solution

[0069] C. utilis cas6 was inoculated on the slant medium and cultured at 28°C for 30 hours to obtain the slant strain. The composition of the slant medium was: glucose 18g / L, peptone 18g / L, yeast extract 8g / L, selenous acid Sodium 25mg / L, agar 15g / L.

[0070] The above slant strains were inoculated into 80 mL of seed culture medium, and cultured with shaking at 28° C., 190 r / min, for 30 hours, to obtain a seed culture solution. The composition of the seed medium is: malt extract 15g / L, peptone 12g / L, yeast extract 8g / L, sodium sel...

Embodiment 3

[0086] The strain composition of the fermented soybean meal compound probiotic bacterial liquid of the present embodiment is as follows: Saccharomyces C.utilis cas65.0×10 9 cfu / ml

[0087] B. licheniforms B.Lincheniforms cas58 3.0×10 9 cfu / ml

[0088] P.pentosaceus cas138 3.0×10 9 cfu / ml

[0089] Lactococcus S.lactis cas168 2.0×10 9 cfu / ml

[0090] The preparation method of the selenium-enriched fermented soybean meal of the present embodiment is as follows:

[0091] (1) Breeding of selenium-enriched strains

[0092] The specific implementation is the same as in Example 1.

[0093] (2) Preparation of seed culture solution

[0094] C. utilis cas6 was inoculated on the slant medium and cultured at 28°C for 32 hours to obtain the slant strain. The composition of the slant medium was: glucose 20g / L, peptone 20g / L, yeast extract 10g / L, selenous acid Sodium 30mg / L, agar 18g / L.

[0095] The above slant strains were inoculated into 80 mL of seed culture medium, and cultured ...

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Abstract

The invention discloses selenium-enriched fermented soybean meal, a preparation method and application. The preparation method comprises the following steps: breeding of a selenium-enriched strain, preparation of seed culture liquid, fermental cultivation, monoclonal fermentation of probiotics, preparation of composite bacteria liquid of the probiotics, adding of a fresh culture medium and preparation of high-activity selenium-enriched soybean meal. According to the preparation method, selenium-enriched yeast and the probiotics are jointly used for fermenting soybean meal, thus obtaining the fermented soybean meal with high organic selenium content and rich amino acids. On one hand, pollution and harm to the environment and an animal body, which are possibly caused by direct adding of a selenite substance into a feed, are avoided; on the other hand, the content of selenium in the soybean meal can be greatly increased; and the selenium-enriched fermented soybean meal is rich in biological organic selenium and also contains a large quantity of beneficial microorganisms and fermentation metabolites, so that the nutrition is richer, the quality of the soybean meal is obviously improved, the ability of an animal for digesting and absorbing nutritional substances can be promoted, the immune function of an organ is enhanced, multifunctional soybean meal with high selenium content is prepared, and the nutritional value and the effect of the soybean meal are increased.

Description

technical field [0001] The invention relates to a fermented soybean meal, in particular to a selenium-enriched fermented soybean meal, a preparation method and an application. Background technique [0002] Selenium is a multifunctional nutritional element necessary to maintain the normal life activities of organisms. The main manifestations of selenium deficiency in animals are white muscle disease and slow growth. The selenium content in natural food is generally low, which cannot meet the needs of normal growth of animals. Selenium-enriched feed can significantly improve animal growth performance, enhance animal reproductive performance, improve animal immune function, and improve the survival rate and disease resistance of pups. At present, the main form of supplementing selenium in animal feed is adding sodium selenite, but sodium selenite has low bioavailability, high toxicity, and is easy to pollute the environment, so its usage is strictly limited. For example, Sweden...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/37A23K50/30A23K50/60A23K10/26
CPCA23V2400/21A23V2400/427Y02P60/87
Inventor 吴丽芳王钰婷黄胜威蔡冬清吴正岩
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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