Preparation method of a bionic vascular network tissue engineering scaffold with multi-level structure

A bionic blood vessel and network tissue technology, which is applied in the fields of medical formula, medical science, prosthesis, etc., can solve the problems of ineffective performance of blood vessel function, easy deformation and blockage, poor mechanical properties, etc., and achieve good scalability, maintenance of survival and Effect of growth, good elasticity

Active Publication Date: 2021-01-01
DONGHUA UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, the gel matrix constructed by 3D printing mentioned above has poor mechanical properties, and it is easy to deform and block under stress without recovery, so it cannot effectively perform the function of its blood vessels.

Method used

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  • Preparation method of a bionic vascular network tissue engineering scaffold with multi-level structure
  • Preparation method of a bionic vascular network tissue engineering scaffold with multi-level structure
  • Preparation method of a bionic vascular network tissue engineering scaffold with multi-level structure

Examples

Experimental program
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Embodiment 1

[0046] (1) 3D printing "sacrificable" sugar template

[0047] ①Model building: use CAD software to construct a model of a cubic network structure, the size is 20mm×20mm, the network gap is designed to be 1.2mm, the intersection angle is 45°, and the design path simulates the path of the nozzle of the 3D printer;

[0048] ②Set printer parameters: Fill the heating chamber of the 3D printer with sucrose powder, set the temperature of the heating chamber to 150°C, the temperature of the nozzle to 135°C, and the heating time to 70 minutes.

[0049] ③Set 3D printing software parameters: layer height is 0.5mm, part position is X-axis 100, Y-axis 100, filling type is 90°, XY-axis movement speed is 1.2mm / s, T2-axis extrusion speed is 0.005mm / s , to get the sugar template.

[0050] (2) Preparation of three-dimensional hollow scaffolds

[0051] ① Preparation of solution: Weigh 0.4g of PCL solid and dissolve it in 10ml of THF, stir magnetically at room temperature for 2 hours to form a ...

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Abstract

The invention relates to a preparation method of a multistage-structure bionic vascular network tissue engineering stent. The preparation method comprises the following steps: (1) establishing a model of a cubic net structure by using CAD software, and then filling a heating chamber of a 3D printer with a sucrose powder to obtain a sucrose template through 3D printing; (2) preparing a polymer material solution, immersing the sucrose template in the step (1) into the polymer material solution, impregnating, taking out, and drying to obtain a polymer coating-containing sucrose template; then removing sucrose from the polymer coating-containing sucrose template through phase separation, and lyophilizing to obtain the multistage-structure bionic vascular network tissue engineering stent. The tissue engineering stent prepared by the preparation method provided by the invention can achieve structural and mechanical double-effect simulation of a vascular network; the preparation method is simple and fast, is suitable for a variety of biological materials, and has a good application prospect.

Description

technical field [0001] The invention belongs to the field of tissue engineering scaffolds, in particular to a method for preparing a bionic vascular network tissue engineering scaffold with a multi-level structure. Background technique [0002] Most of the existing tissue engineering constructs do not have an ideal microvascular network, and their diffusion exchange ability and material transport performance are poor. The high-density cells in the tissue cannot obtain sufficient material exchange and quickly form necrotic centers, making it difficult to construct thick and dense structures. The tissue especially maintains its long-term activity [J.P.Armstrong, R.Shakur, J.P.Horne, S.C.Dickinson, C.T.Armstrong, K.Lau, J.Kadiwala, R.Lowe, A.Seddon, S.Mann, J.L.Anderson, A.W. Perriman and A.P.Hollander, Nat Commun, 2015, 6, 7405.]. Therefore, it is a recognized difficulty and challenge in the field of tissue engineering to construct an engineered tissue that contains a bionic ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/18A61L27/58A61L27/56A61L27/50
CPCA61L27/18A61L27/50A61L27/56A61L27/58A61L2400/08C08L67/04
Inventor 游正伟雷东
Owner DONGHUA UNIV
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