Protein/polypeptide-polymer conjugate with fluorescence emission properties and its preparation method and application
A fluorescent emission, polymer technology, applied in the direction of albumin peptides, animal/human proteins, medical preparations with inactive ingredients, etc., can solve problems such as preventing real-time monitoring
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0068] The preparation method of the vesicles in the present invention is not particularly limited, and may be a preparation method well known to those skilled in the art. Preferably in the present invention, the above polypeptide-polymer conjugate is dissolved in DMSO, added with deionized water, and then dialyzed with deionized water.
[0069] The polymer vesicles provided by the present invention are uniformly dispersed and have a uniform particle size distribution, and the particle size distribution is 60-150 nm.
[0070] The above-mentioned polymersomes have matrix metalloenzyme responsive properties, so they can be used as drug carriers, or as fluorescent indicators to monitor drug release in real time.
Embodiment 1c1
[0074] The synthesis of embodiment 1c1
[0075] 4-Bromosalicylaldehyde (1; 3.11g, 15.4mmol), Pd(PPh 3 ) 2 Cl 2 (0.22g, 0.31mmol), PPh 3 (0.061g, 0.23mmol), anhydrous tetrahydrofuran (50mL) was added to a reaction flask containing a magnetic stirrer, and the reaction system was degassed by bubbling with dry nitrogen for 30min, and then freshly evaporated dry Et 3 N (3.05 g, 30.0 mmol) and trimethylethynylsilane (1.67 g, 17.0 mmol) were added under nitrogen atmosphere, the solution turned orange. After stirring for 20 min, the cocatalyst CuI (0.088 g, 0.46 mmol) was added to the reaction system under a nitrogen atmosphere, and the solution turned dark brown. After stirring overnight at room temperature, the solvent was removed to give a dark brown solid, which was dissolved in n-pentane and filtered to give a yellow solution. All the solvents were spinned off, and finally, recrystallized twice in n-hexane to obtain yellow crystal 2 (2.96g, yield: 88.2%, >95% purity by HPLC)...
Embodiment 2
[0087] Example 2 BSA and PEG mediated by bifunctional fluorescent molecule c1 227 -N 3 Fluorescent conjugates of
[0088] Bovine serum albumin BSA (498 mg, 7.5 μmol) was dissolved in phosphate buffer (PBS) (70 mL, pH 6.5, 0.1 M, containing 1 mM EDTA), and tris (2-chloroethyl) phosphate hydrochloride (TCEP· HCl) (21.5 mg, 75 μmol) was dissolved in PBS (2.5 mL), and then added dropwise to the above BSA solution. After 4 h, the solution was dialyzed against deionized water for 24 h (2.0 kDa molecular weight cut-off), and then lyophilized to obtain BSA red .
[0089] BSA or BSA red (4mg, 0.06μmol) dissolved in PBS (0.9mL, pH 7.0, 50mM), C1 (0.6μmol, dissolved in 0.1mL DMSO), PEG 227 -N 3 (6mg, 0.6μmol) and CuSO 4 / Na-ascorbate (1 / 5 molar ratio) was added to the solution, stirred at 25°C for different times, and the conjugation progress was detected by in situ observation of the change of fluorescence emission intensity at about 420 nm. After reaching the set time, 80 μL of ...
PUM
| Property | Measurement | Unit |
|---|---|---|
| particle diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More