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Beauveria bassiana strain EHM-068 and application thereof

A technology of EHM-068, Beauveria bassiana, applied in application, fungi, biocides and other directions, can solve problems such as unfavorable biological control of Beauveria bassiana, and achieve difficult drug resistance, convenient use and good control effect Effect

Active Publication Date: 2017-07-21
JILIN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, when using Beauveria bassiana for field pest control, it is often inconvenient to a certain extent because of the production of corresponding specialized strains for different target pests, which is not conducive to the further promotion of biological control using Beauveria bassiana

Method used

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  • Beauveria bassiana strain EHM-068 and application thereof
  • Beauveria bassiana strain EHM-068 and application thereof
  • Beauveria bassiana strain EHM-068 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Isolation and identification of Beauveria bassiana bacterial strain EHM-068

[0027] (1) Separation and purification

[0028] After fully ossification of the naturally infected rice water weevil adults collected from the paddy fields of Gongzhuling City, Jilin Province, the white powder on the body surface was gently shaken off to contain 0.1% Tween-80 sterilized aqueous solution Shake the spores of Beauveria bassiana evenly in a sterilized Erlenmeyer flask, take the prepared spore suspension for gradient dilution, and use a sterile applicator dipped in different dilution times of Beauveria bassiana spores to spread evenly on the Place the culture on the PDA medium plate in an environment with a temperature of 26° C. and a relative humidity of 90%.

[0029] When a single colony grows, use a sterile inoculation needle to pick the colony and transfer it to another new PDA medium plate for separate culture. After continuing to culture and confirm that there are ...

Embodiment 2

[0042] The biological characteristic index of embodiment 2 Beauveria bassiana bacterial strain EHM-068

[0043] The spore yield, spore germination rate, strain growth rate and pathogenicity of Beauveria bassiana strains are important indicators reflecting the excellent traits of the strain.

[0044] (1) Determination of spore production

[0045] The sporulation yield of a strain reflects the reproductive ability of the strain, the dispersal ability and range of spores in the environment, and is one of the important indicators to measure the quality of a strain.

[0046] First, the Beauveria bassiana strains were prepared with 0.1% Tween-80 sterile water to a concentration of 1x10 7 individual / mL conidia suspension. Inoculate 200 uL of conidia suspension on the PDA solid plate medium, spread evenly with a sterilized glass rod, and then place it at a temperature of 25°C and a relative humidity of 90% to cultivate for 10-15 days. Use a hole puncher with a diameter of 0.8cm to ...

Embodiment 3

[0079] Example 3 Using the insecticide prepared by Beauveria bassiana strain EHM-068 to control Chilo borer and Rice water weevil

[0080] 1) Field test method:

[0081] From May to June 2016, the rice field was selected as the test base in Gongzhuling City, Jilin Province. The area of ​​the rice field is 300m 2 , a total of six treatments were set up in this experiment, arranged randomly. Handling one to four all adopts the insecticide containing Beauveria bassiana strain EHM-068 provided by the invention, wherein the content of conidia of Beauveria bassiana strain EHM-068 bacterial strain in the insecticide is 5,000,000,000 spores / g Bacteria agent, the spraying amount of treatment 1 to 4 is 62.5g / mu.time, 125g / mu.time, 250g / mu.time and 500g / mu.time respectively, treatment 5 is sprayed with biological pesticide, and treatment 6 is sprayed with water as a control test. Treatments 1 to 6 were evenly sprayed on the stems and leaves of the paddy field with a knapsack sprayer, ...

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Abstract

The invention relates to the technical field of biological control of crop pests, in particular to a beauveria bassiana strain EHM-068 and application thereof. The strain is preserved in the common microorganism center of Chinese microorganism strain preservation management committee on March 30, 2016, and the preservation number is CGMCC No.12110. The invention further provides application of the beauveria bassiana strain EHM-068 in prevention and control of rice-stem borer and lissorhoptrus oryzophilus and application in preparation of pesticides. The beauveria bassiana strain EHM-068 can simultaneously have the high pathogenicity to the rice-stem borer and lissorhoptrus oryzophilus, and the rice-stem borer and lissorhoptrus oryzophilus preventing and controlling effect is remarkably improved.

Description

technical field [0001] The invention relates to the technical field of biological control of crop pests, in particular to a Beauveria bassiana strain EHM-068 and its application. Background technique [0002] Chilo suppressalis Walker (Chilo suppressalis Walker), which belongs to Lepidoptera and Mothidae, is one of the most serious frequent pests of rice in my country. In addition to harming rice, Chilo borer can also harm weeds such as water bamboo, corn, sorghum, sugar cane, rapeseed, broad bean, wheat, reed, barnyardgrass, and Lishihe. Chilo borer larvae damage rice leaf sheaths, heart leaves, rice stems and other parts to cause damage such as withered sheaths, dead heart seedlings, and white ears. In the mature stage, they will cause semi-dead spike insects to damage the plants, resulting in severe yield reduction. Chilo borer can generally lead to a 5%-10% reduction in rice yield, and in severe cases it can be as high as 20%-30%. [0003] Rice water weevil (Lissorhopt...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P7/02C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 田志来朱晓敏张强
Owner JILIN ACAD OF AGRI SCI
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