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L-phenylalanine and preparation method thereof

A technology for phenylalanine and liquid ammonia, applied in the field of L-phenylalanine and its preparation, can solve the problems of low yield, multiple impurities in L-phenylalanine, etc., so as to increase the volume of the tank and ensure nutrition. Ingredient, foam reducing effect

Inactive Publication Date: 2017-07-21
新疆苏源生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many preparation methods for L-phenylalanine, such as protein hydrolysis extraction, direct fermentation, chemical synthesis, etc., but the L-phenylalanine prepared by the current method contains more impurities and the yield is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1 proposes a kind of preparation method of L-phenylalanine, comprises the steps:

[0019] (1) add water and the first substratum in batching tank, stir evenly, obtain the first culture solution, then put the first culture solution into the seed tank, then add NaOH to adjust its pH value to 7.0, and then Sterilize at 115°C for 8 minutes, then cool it to 33.0°C, add 2.0 parts by weight of sterilized liquid glucose, then add liquid ammonia to adjust its pH value to 6.3, and then inoculate at 33.0°C Escherichia coli was cultured for 16 hours; wherein, the first culture medium included the following raw materials in parts by weight: 1.0 part of peptone, 0.5 part of yeast extract, 0.5 part of sodium chloride, 0.2 part of ammonium sulfate, 0.001 part of vitamin B1 and kanamycin 0.004 part; The weight ratio of described water and described first culture medium is 100:4.5, and the mass concentration of described liquid glucose is 65%, and the insertion amount of descr...

Embodiment 2

[0024] Embodiment 2 proposes a kind of preparation method of L-phenylalanine, comprises the steps:

[0025] (1) add water and the first substratum in batching tank, stir evenly, obtain the first culture solution, then put the first culture solution into the seed tank, then add NaOH to adjust its pH value to 7.2, and then Sterilize at 110°C for 12 minutes, then cool it to 32.0°C, add 2.0 parts by weight of sterilized liquid glucose, then add liquid ammonia to adjust its pH to 6.7, and inoculate at 32.0°C Escherichia coli was cultured for 18 hours; wherein, the first culture medium included the following raw materials in parts by weight: 1.0 part of peptone, 0.5 part of yeast extract, 0.5 part of sodium chloride, 0.2 part of ammonium sulfate, 0.001 part of vitamin B1 and kanamycin 0.004 part; The weight ratio of described water and described first culture medium is 100:4.5, and the mass concentration of described liquid glucose is 65%, and the insertion amount of described esche...

Embodiment 3

[0030] Embodiment 3 proposes a kind of preparation method of L-phenylalanine, comprises the steps:

[0031](1) add water and the first substratum in batching tank, stir evenly, obtain the first culture solution, then put the first culture solution into the seed tank, then add NaOH to adjust its pH value to 7.1, and then Sterilize at 112°C for 10 minutes, then cool it to 32.0°C, add 2.0 parts by weight of sterilized liquid glucose, then add liquid ammonia to adjust its pH to 6.5, and inoculate at 33.0°C Escherichia coli was cultured for 17 hours; wherein, the first culture medium included the following raw materials in parts by weight: 1.0 part of peptone, 0.5 part of yeast extract, 0.5 part of sodium chloride, 0.2 part of ammonium sulfate, 0.001 part of vitamin B1 and kanamycin 0.004 part; The weight ratio of described water and described first culture medium is 100:4.5, and the mass concentration of described liquid glucose is 65%, and the insertion amount of described escher...

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PUM

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Abstract

The invention relates to L-phenylalanine and a preparation method thereof. The preparation method includes the following steps that water and a first culture medium are added into a mixing tank, a first culture solution is obtained and pumped into a seed tank, then, the pH value of the first culture solution is adjusted, sterilization is conducted, then, cooling is conducted, liquid glucose is added, the pH value of a mixture of the liquid glucose and the first culture solution is adjusted, and the mixture is inoculated with escherichia coli; liquid glucose is pumped into a carbohydrate consumption tank, constant volume operation is conducted, and then sterilization and cooling are conducted; dipotassium phosphate and monopotassium phosphate are put into a material supplementing tank, constant volume operation is conducted, and then sterilization and cooling are conducted; water and a second culture medium without glucose are pumped into the mixing tank, a second culture solution is obtained, then, the second culture solution is added into a fermentation tank and sterilized, then, the temperature of the second culture solution is reduced to 31-33 DEG C, sterilized liquid glucose is added, then, the pH value of a mixture of the second culture solution and the sterilized liquid glucose is adjusted, strains in the seed tank are transplanted in the fermentation tank to be fermented, and L-phenylalanine is obtained. By the adoption of the preparation method of L-phenylalanine, harmful pigment in a fermentation medium is reduced, and the acid production and the fermentation level are improved.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to L-phenylalanine and a preparation method thereof. Background technique [0002] L-Phenylalanine is a colorless to white flaky crystal or white crystalline powder. It is a nutritional supplement and one of the essential amino acids. L-phenylalanine is an important food additive-the main raw material of the sweetener aspartame, one of the essential amino acids for the human body, and is mainly used in amino acid infusions and amino acid drugs in the pharmaceutical industry. L-Phenylalanine is an essential amino acid that cannot be synthesized by the human body. There are many methods for preparing L-phenylalanine, including protein hydrolysis extraction, direct fermentation, chemical synthesis, etc. However, the L-phenylalanine prepared by the current method contains more impurities and has a lower yield. Contents of the invention [0003] One object of the pre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/22C12N1/20C12R1/19
CPCC12P13/222C12N1/20
Inventor 张伟陈飞翔校军锋李涛陶武装章江吴鹏王成
Owner 新疆苏源生物工程有限公司
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