Second harmonic imaging method and second harmonic imaging device for single suspended particles

A second harmonic, imaging method technology, applied in the field of optical imaging, can solve problems such as insufficient resolution, difficulty in PM2.5 particles, inability to distinguish condensed water and condensation nuclei, and achieve high capture efficiency and low cost Effect

Inactive Publication Date: 2017-07-28
NORTHWEST UNIV
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Problems solved by technology

However, because the resolution of traditional optical microscopy imaging is not high enough to distinguish condensation water and condensat

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  • Second harmonic imaging method and second harmonic imaging device for single suspended particles

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Example Embodiment

[0029] Example 1

[0030] This embodiment provides a second harmonic imaging method for a single suspended particle, including the following steps:

[0031] Step 1: A Bessel beam is incident on a sample chamber 9 containing a single fine particle along an optical path, and a Bessel beam standing wave field is generated in the sample chamber by interference; the diameter of the fine particle is smaller than 10μm; The obtained Bebessel beam standing wave field can capture the three-dimensional information of fine particles;

[0032] Step 2: Focus a femtosecond laser on the sample chamber so that the femtosecond laser and the Bessel beam are collinear. The fundamental frequency light and the second harmonic signal emitted from the sample chamber are separated and imaged separately; The captured fine particles interact to produce a second harmonic signal;

[0033] Step 3: Adjust the size of the bright spot in the center of the Bessel beam until a stable fine particle image appears on the...

Example Embodiment

[0035] Example 2

[0036] The second harmonic imaging device for single suspended particles provided by the present invention, see figure 1 , Including femtosecond laser 1, continuous laser 2, laser beam expander 3, Bessel beam generator 4, first mirror 5, second mirror 6, convex lens 7, first microscope objective lens 8, sample chamber 9 , The second microscope objective lens 10, the third mirror 11, the first focusing lens 12, the first imaging device 13, the filter 14, the second focusing lens 15, the second imaging device 16, the rear quartz window 17, the front quartz Window 18.

[0037] Among them, the femtosecond laser 1 is a laser whose wavelength is adjustable in the range of 750-990nm; the center wavelength of the continuous laser 2 is 532nm; the sample chamber 9 contains free-floating fine particles (less than 10μm in diameter); laser beam expander 3 The beam expansion magnification can be adjusted within 2-10 times; the back focus of the convex lens 7 coincides with t...

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Abstract

The invention discloses a second harmonic imaging method and a second harmonic imaging device for single suspended particles. According to the method, Bessel laser is adopted for effectively capturing the suspended particles, and remote noncontact and noninvasive capture and high capture efficiency are realized; imaging is realized by second harmonic signals generated on the particles by femtosecond laser, polarization resolution, spectral resolution and three-dimensional imaging are realized, and imaging and observation of fine particles are realized. The device for implementing the second harmonic imaging method has advantages that due to adoption of common optical devices, low cost is realized while imaging of the second harmonic signals can be well realized, and suitableness for popularization is achieved.

Description

technical field [0001] The invention belongs to the field of optical imaging, and in particular relates to a second harmonic imaging method and device suitable for single suspended particles based on laser capture. Background technique [0002] Particulate pollutants floating in the atmosphere are not only one of the important factors causing environmental problems such as reduced atmospheric visibility and photochemical smog, but more seriously, fine particulate matter (PM2.5) with a diameter of less than 2.5 μm can enter the human body through the respiratory system and penetrate deeply Lungs and cardiovascular system, causing damage to the respiratory system and cardiovascular system. Studies have established that PM2.5 pollutants directly contribute to asthma, lung cancer, cardiovascular disease, birth defects and premature death. At present, PM2.5 pollution has become the most prominent atmospheric environmental problem in our country, seriously threatening the life an...

Claims

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Application Information

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IPC IPC(8): G01N21/63G01N21/01
CPCG01N21/01G01N21/63
Inventor 程雪梅陈浩伟张倩任兆玉白晋涛
Owner NORTHWEST UNIV
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