Fermentation parameters of porcine colonic microbiome in vitro simulation culture

Abstract

The invention discloses fermentation parameters for in-vitro simulated culture of a porcine colon microbiome. The fermentation parameters include a culture medium for in-vitro simulated culture of porcine intestinal microorganisms. The culture medium comprises a solute which is formed by beef extract powder, tryptose, L-cysteine-HCL, glucose, yeast extract powder, sodium chloride, soluble starch and molasses. The fermentation parameters further include the following parameters: the volume of fermenting liquid is 330mL, the pH value is 6.4+ / -0.1, the temperature is 37+ / -0.1 DEG C, the stirring speed is 120rpm, the supplementing speed and the discharging speed are 330mL / day, the fermentation time is 12 days, and the environment is a strict anaerobic environment. By optimizing a culture medium of an in-vitro continuous fermentation culture medium, the microbiome with relatively high similarity with a porcine intestinal tract is cultured, so that a reliable and powerful practical tool is provided for in-vitro study of the porcine intestinal microorganisms, and the production of pigs and the study of human health are positively promoted.

Description

technical field [0001] The present invention relates to the fields of biotechnology and animal husbandry, and in particular to a fermentation parameter for in vitro simulated culture of porcine colon microbiome, which is mainly a culture medium formula, and also includes fermentation liquid volume, pH value, fermentation time, temperature, stirring speed, oxygen partial pressure, Feed and discharge speeds. Background technique [0002] There are a large number of microorganisms living in the gut of animals, the number and number of genes of which exceed those of the host (Baekhed et al., 2005; Qin et al., 2010), and are closely associated with the host. Colonic microbes dominate in abundance, variety, and function. Intestinal microorganisms have important functions in nutrition, immunity, anti-tumor and anti-aging (Wang Shanshan et al., 2015). Microbes can not only ferment substrates to provide the host with 10% of the energy required by the host, but also synthesize vitami...

AI Technical Summary

Problems solved by technology

[0011] Solve the problem of poor in vitro simulation culture of pig colonic microbiome, make the similarity between the simulated cultured intestinal microbiome and colonic microbiome reach a higher level, and make the in vitro fermentation simulation culture system replace animal experiments to study pig colonic microorganisms more reliable , credible

Images