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A method of combining ultra-low temperature and pulverization to pretreat peanut shells as a supplementary substrate for biohydrogen production

A technology of biological hydrogen production and peanut shells, applied in the field of biochemistry, can solve the problems of corrosive equipment, long processing cycle, and environmental pollution, and achieve the effects of small environmental pollution, short cycle, and low energy consumption

Active Publication Date: 2020-12-11
NORTHEASTERN UNIV LIAONING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, common cellulose pretreatment methods include physical methods, chemical methods, biological methods, etc. Among them, physical methods such as ultrasonic method and ultraviolet method have little environmental pollution, but consume a lot of energy; chemical methods such as acid treatment and alkali treatment etc., the effect is good, but it is easy to corrode equipment and pollute the environment; biological methods such as white rot fungi, brown rot fungi, etc., are non-polluting, but the treatment cycle is long

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The method of using ultra-low temperature freezing and crushing peanut shells as a supplementary substrate to ferment hydrogen production in this embodiment is carried out according to the following steps:

[0025] Step 1: Take the peanut shells sold in the market, wash off the dust on the surface with water, put them in the oven, dry them at 60°C until they reach a constant weight, put them at -40°C, freeze them at ultra-low temperature for 36 hours, and take them out quickly Put it in a plant pulverizer and pulverize to 40 mesh.

[0026] Step 2: Add glucose anaerobic fermentation medium (ingredients include: glucose 10g / L, peptone 4g / L, yeast powder 1g / L, L-cysteine ​​0.5g / L, Na 2 HPO 4 12H 2 O 14.33g / L, KH 2 PO 4 3.63g / L, MgSO 4 ·7H 2 (0.37g / L) 50mL and the peanut shells after the above-mentioned pretreatment, meanwhile, get untreated peanut shells and add in the drop bottle as a control group, wherein the peanut shell content in the medium is 3g, use high-pur...

Embodiment 2

[0031] The method of using ultra-low temperature freezing and crushing peanut shells as a supplementary substrate to ferment hydrogen production in this embodiment is carried out according to the following steps:

[0032] Step 1: Take the peanut shells sold in the market, wash off the dust on the surface with water, put them in an oven, dry them at 60°C until they reach a constant weight, then place them at -60°C, freeze them at ultra-low temperature for 24 hours, and quickly Take it out and put it in a plant pulverizer, and pulverize it to 60 mesh.

[0033] Step 2: Add glucose anaerobic fermentation medium (ingredients include: glucose 10g / L, peptone 4g / L, yeast powder 1g / L, L-cysteine ​​0.5g / L, Na 2 HPO 4 12H 2 O 14.33g / L, KH 2 PO 4 3.63g / L, MgSO 4 ·7H 2 O 0.37g / L) 50mL and the above-mentioned peanut shells after pretreatment, wherein the content of peanut shells is 0, 1, 2, 3, 4, 5g respectively, blow off with high-purity nitrogen to discharge oxygen, and under the c...

Embodiment 3

[0038] The method of using ultra-low temperature freezing and crushing peanut shells as a supplementary substrate to ferment hydrogen production in this embodiment is carried out according to the following steps:

[0039] Step 1: Take the peanut shells sold in the market, wash off the dust on the surface with water, put them in an oven, dry them at 60°C until they reach a constant weight, then place them at -80°C, freeze them at ultra-low temperature for 48 hours, and quickly Take it out and put it in a plant pulverizer, and pulverize it to 80 mesh.

[0040] Step 2: Add glucose anaerobic fermentation medium (ingredients include: glucose 10g / L, peptone 4g / L, yeast powder 1g / L, L-cysteine ​​0.5g / L, Na 2 HPO 4 12H 2 O 14.33g / L, KH 2 PO 4 3.63g / L, MgSO 4 ·7H 2 (0.37g / L) 50mL and the peanut shells after the above-mentioned pretreatment, wherein the peanut shell content is 2g, adjust the pH to be 5.5, 6.0, 6.5, 7.0, 7.5, 8.0 respectively, use high-purity nitrogen to blow off ...

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Abstract

The invention discloses a method for pretreating peanut shells by combining ultra-low temperature and crushing as a supplementary substrate for biological hydrogen production, which is characterized by: placing the dried peanut shells in an ultra-low temperature environment for a period of time, and then quickly crushing them physically after taking them out; Place the above-processed peanut shells together with the glucose anaerobic fermentation medium in a culture bottle, fill it with nitrogen, and sterilize it at high temperature; add the activated anaerobic hydrogen production medium to the above mixed glucose anaerobic fermentation medium. The bacteria are placed under suitable conditions to carry out anaerobic biological fermentation to produce hydrogen. The invention has the following advantages: (1) It helps to recycle peanut shells and avoids the environmental problems caused by traditional treatment methods; (2) It helps to destroy its lignin protective layer and improve the accessibility of cellulose. area, reducing the crystallinity, thereby improving its hydrogen production efficiency; (3) Provides a cellulosic biomass pretreatment method with low environmental pollution, short cycle, and low energy consumption.

Description

technical field [0001] The invention relates to the field of biochemistry, specifically belongs to the fields of resource utilization of agricultural waste and hydrogen production by microbial fermentation, and relates to a method for pretreating peanut shells by combining ultra-low temperature freezing and crushing as a supplementary substrate for biological hydrogen production. Background technique [0002] As a clean and high-combustion energy source, hydrogen energy has broad application prospects. However, natural hydrogen cannot be utilized as an effective source of hydrogen energy. Therefore, the development of economical and efficient hydrogen production methods has become one of the urgent problems to be solved. [0003] As a new type of hydrogen production technology, biological fermentation hydrogen production technology has the advantages of low energy consumption and clean products compared with traditional hydrogen production technologies (such as electrolysis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P3/00
CPCC12P3/00
Inventor 赵鑫齐男胡筱敏杨静许淑红李丹阳姜彬慧
Owner NORTHEASTERN UNIV LIAONING