Serum protein marker group for diagnosis of type 1 and type 2 diabetes mellitus

A type 2 diabetes and serum protein technology, applied in the field of diabetes diagnostic protein markers, can solve the problems of diagnosis deviation and poor treatment effect, and achieve reliable and accurate diagnosis results

Inactive Publication Date: 2017-08-08
XINJIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Diagnostic bias is one of the main c...

Method used

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  • Serum protein marker group for diagnosis of type 1 and type 2 diabetes mellitus
  • Serum protein marker group for diagnosis of type 1 and type 2 diabetes mellitus
  • Serum protein marker group for diagnosis of type 1 and type 2 diabetes mellitus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Differentially expressed proteins in patients with MODY and healthy controls detected by iTRAQ technology

[0036] 1. Test samples:

[0037] A four-generation surviving Uyghur family with premature diabetes. Divided into 4 cases of case and 4 cases of normal control serum. Collect 2mL of whole blood on an empty stomach in the morning, let it stand at 4°C for 1-2h until the blood coagulates and precipitate the serum, centrifuge at 3000g for 10min, collect the supernatant, aliquot it on ice and store it at -80°C for later use.

[0038] 2. Detection method:

[0039] (1) Use the Proteominer kit to remove high-abundance proteins in serum: add DTT with a final concentration of 10mM to the enriched protein sample, and bathe in water at 56°C for 1h; after cooling to room temperature, add IAM with a final concentration of 55mM, and place in a dark room for 45min Add 1mL of cold acetone to precipitate overnight, centrifuge at 25000rpm*4°C for 15min and discard the supernatant;...

Embodiment 2

[0059] Validation of Mass Spectrometry MRM in MODY Family

[0060] 1. Test samples:

[0061] MODY patients, 4 cases of normal control serum; all from the family

[0062] 2. Detection method:

[0063] The establishment of the experimental method 1) Select the MODY-related target protein suitable for MRM detection and analysis; 2) Evaluate the quality of the extracted protein; 3) Select the parent-child ion pair suitable for MRM detection; 4) Analyze the mass spectrum based on the analysis software Skyline 5) Perform quality assessment and analysis on the obtained detection data. Sample Analysis:

[0064] (1) Serum proteolysis: 12.5ug of each sample was taken, combined into two tubes of MODY case group and healthy control group, and 01ug / ul of BSA was added to each tube as an internal reference. Use an ultrafiltration tube, take the protein solution, centrifuge for 20min, remove the bottom solution, add 100ul TEAB, centrifuge as above, repeat the step 3 times, replace a new ...

Embodiment 3

[0074] 1. Test samples:

[0075] Type 1 diabetes patients, type 2 diabetes patients, 10 cases of healthy control serum;

[0076] 2. Detection method:

[0077] The establishment of the experimental method 1) MRM detection and analysis of the selected parent-child ion pairs in the expanded 30 samples; 2) Based on the analysis software Skyline, the mass spectrometer scanning parameters-collision energy were optimized; 4) The obtained detection Data quality assessment and analysis.

[0078] Sample Analysis:

[0079] (1) Serum proteolysis: 200ug was taken from each sample, and 0.1ug / ul BSA was added to each tube as an internal reference. Add DTT with a final concentration of 10mM to the taken original serum sample, and put it in a water bath at 56°C for 1h; after cooling to room temperature, add IAM with a final concentration of 55mM, and place it in a dark room for 45min; add TEAB with a final concentration of 100Mm, according to the protein:enzyme=40: Add Trypsin enzyme at a ...

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Abstract

The invention relates to a serum protein marker group for diagnosis of type 1 and type 2 diabetes mellitus. The serum protein marker group is composed of the following serum protein markers: PZP, which has an Accession number of P20742, sex hormone binding globulin: SHBG, which has an Accession number of I3L145, C reactive protein: CRP, which has an Accession number of P02741, cholesteryl ester transfer protein: CETP, which has an Accession number of P11597, and a heparin cofactor 2: SERPIND1, which has an Accession number of P05546.

Description

technical field [0001] The invention relates to the field of diabetes diagnostic protein markers, in particular to a serum protein marker group for diagnosing type 1 and type 2 diabetes. Background technique [0002] As a special type of diabetes caused by a single gene mutation, MODY has attracted the attention of scholars from various countries in recent years. Because MODY has the characteristics of early onset age, autosomal dominant inheritance, and high penetrance, it is conducive to the collection of multi-generation families. Therefore, in other words, MODY families provide an ideal for the study of molecular genetic etiology and pathogenesis of diabetes. research object. MODY is easily misdiagnosed as type 1 or type 2 diabetes clinically, resulting in differences in its treatment, so the correct diagnosis of MODY is imminent. It is also necessary to strengthen the understanding of diabetes types, and constantly improve relevant clinical detection indicators, so th...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2800/042
Inventor 帕它木·莫合买提先锋伊力哈木江·依马木热沙来提·阿不都瓦衣特木哈达斯·吐尔逊依明热比亚·努力伊再提古丽·木提拉祖力卡提阿衣·阿布都拉阿瓦古丽·托合提艾扎提古丽·卡的尔
Owner XINJIANG MEDICAL UNIV
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