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Probiotic and preparation method thereof

A technology of probiotics and bacterial liquid, applied in the field of microorganisms, can solve the problems of complex separation and single type of probiotics, and achieve the effect that the preparation method is simple and easy to operate, and is suitable for large-scale promotion and application

Active Publication Date: 2017-08-15
GUANGZHOU KANGZE MEDICAL TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the present invention provides a kind of probiotics and its preparation method, which is used to solve the technical defects of single type of probiotics and complex separation in the prior art

Method used

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  • Probiotic and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] This embodiment is a specific embodiment for preparing probiotics.

[0030] 1.1 Mucin purification

[0031] 1.1.1 Material preparation

[0032] Weigh 0.2g anhydrous NaH 2 PO 4 , 7gNa 2 HPO 4 12H 2 Dissolve O and 6g NaCl in deionized water, adjust the pH to 7.8, set the volume to 1000ml, and sterilize under high pressure at 125°C for 30min to prepare liquid A, which is stored at room temperature.

[0033] Weigh 5.85g of NaCl in deionized water, adjust the pH to 7.0, set the volume to 1000ml, sterilize under high pressure at 125°C for 30min to prepare liquid B, and store at room temperature.

[0034] 1.1.2 Purification

[0035] Weigh 10-15g of mucin powder, add it into a 500ml flask of liquid A, stir evenly on a magnetic stirrer for 2 hours, adjust the pH of the solution to 7.2±0.2 with 1M sodium hydroxide, add 500-1000ul of toluene dropwise, and continue to Stir evenly on a stirrer for 18 hours, centrifuge at 3000 rpm for 10 minutes, collect the supernatant into ...

Embodiment 2

[0068] This example is a specific example of verifying that the purified colony prepared in Example 1 participates in carbohydrate metabolism.

[0069] 2.1 DNA extraction, library construction and sequencing

[0070] Bacterial DNA was extracted using a mouse small-dose fecal DNA extraction kit (Meiji Biology, Guangzhou), according to the product instructions and technical means well known to those of ordinary skill in the art.

[0071] Using the TruSeq DNA sample preparation kit, according to the product instructions and technical means known to those of ordinary skill in the art, a DNA library with a paired end of 150 bp and an insert length of 250-300 bp was constructed.

[0072] Illumina Hiseq2500 sequencer was used for DNA sequencing, and high-quality sequencing fragments were extracted for follow-up work such as bacterial genome assembly, annotation, and comparative genome analysis.

[0073] 2.2 Genome assembly

[0074] The sequencing fragments were assembled using the ...

Embodiment 3

[0081] This example is a specific example of verifying that the purified colony prepared in Example 1 participates in fat metabolism.

[0082] 3.1 DNA extraction, library construction and sequencing

[0083] Bacterial DNA was extracted using a mouse small-dose fecal DNA extraction kit (Meiji Biology, Guangzhou), according to the product instructions and technical means well known to those of ordinary skill in the art.

[0084] Using the TruSeq DNA sample preparation kit, according to the product instructions and technical means known to those of ordinary skill in the art, a DNA library with a paired end of 150 bp and an insert length of 250-300 bp was constructed.

[0085] Illumina Hiseq2500 sequencer was used for DNA sequencing, and high-quality sequencing fragments were extracted for follow-up work such as bacterial genome assembly, annotation, and comparative genome analysis.

[0086] 3.2 Genome Assembly

[0087]The sequencing fragments were assembled using the Velvet mic...

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Abstract

The invention belongs to the technical field of microorganisms and particularly relates to a probiotic and a preparation method thereof. The invention provides the probiotic, and the preservation number of the probiotic is GDMCC60085. The invention further provides a preparation method of the probiotic. The preparation method comprises the steps of (1) preparing a bacteria solution; (2) carrying out PCR amplification; and (3) cultivating and purifying a bacterial colony. The invention further provides a probiotic solid yeast, wherein the probiotic solid yeast is prepared from the probiotic. According to the technical scheme, through a whole-genome sequencing experiment, the prepared strain can participate in carbohydrate metabolism and fat metabolism, meanwhile, the vitamin and short-chain fatty acid acetic acid can be produced, and the probiotic is the probiotic with good potential. Meanwhile, the preparation method is simple and easy to operate, and suitable for large-scale popularization and application. The technical problems of a single probiotic variety and complicated separation of the prior art are solved.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a probiotic and a preparation method thereof. Background technique [0002] Probiotics are a class of active microorganisms that are beneficial to the host. They are a general term for active beneficial microorganisms that colonize the human intestinal tract and reproductive system and can produce definite health effects to improve the host's micro-ecological balance and exert beneficial effects. Probiotics play an important role in the health of the host, mainly including the following aspects: 1. Help the host digest and absorb nutrients; 2. Produce important substances such as vitamins, short-chain fatty acids and antioxidants; 3. By inhibiting the growth of harmful bacteria Growth and removal of toxins produced by harmful bacteria to help the host resist bacterial and viral infections and enhance the host's resistance. [0003] At present, the probiotics d...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20C12N1/205C12R2001/01
Inventor 彭永正伍菲凡郭先凤易江丰
Owner GUANGZHOU KANGZE MEDICAL TECH CO LTD
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