Probiotic and preparation method thereof

A technology of probiotics and bacterial liquid, applied in the field of microorganisms, can solve the problems of complex separation and single type of probiotics, and achieve the effect that the preparation method is simple and easy to operate, and is suitable for large-scale promotion and application

Active Publication Date: 2017-08-15
GUANGZHOU KANGZE MEDICAL TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the present invention provides a kind of probiotics and its preparation method, whic

Method used

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  • Probiotic and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0028] Example 1

[0029] This example is a specific example for preparing probiotics.

[0030] 1.1 Mucin purification

[0031] 1.1.1 Material preparation

[0032] Weigh 0.2g anhydrous NaH 2 PO 4 , 7gNa 2 HPO 4 ·12H 2 O and 6g NaCl were dissolved in deionized water, adjusted to pH 7.8, fixed to 1000ml, sterilized under high pressure at 125°C for 30 minutes to prepare liquid A, and stored at room temperature.

[0033] Weigh 5.85g NaCl in deionized water, adjust the pH to 7.0, dilute the volume to 1000ml, and sterilize it under high pressure at 125°C for 30 minutes to prepare liquid B, and store it at room temperature.

[0034] 1.1.2 Purification

[0035] Weigh 10-15g of mucin powder and add it to a 500ml flask of solution A. After stirring for 2h on a magnetic stirrer, adjust the pH of the solution to 7.2±0.2 with 1M sodium hydroxide. Add 500-1000ul of toluene dropwise and continue to Stir uniformly on a stirrer for 18 hours, centrifuge at 3000 rpm for 10 minutes, collect the supernatant ...

Example Embodiment

[0067] Example 2

[0068] This example is a specific example to verify that the purified colony prepared in Example 1 participates in carbohydrate metabolism.

[0069] 2.1 DNA extraction, library construction and sequencing

[0070] Using the mouse low-dose fecal DNA extraction kit (Meiji Biotech, Guangzhou), the bacterial DNA was extracted according to the product instructions and technical means well known to those of ordinary skill in the art.

[0071] Using the TruSeq DNA sample preparation kit, according to the product instructions and technical means well known to those of ordinary skill in the art, a DNA library with double-end 150bp and insert length 250-300bp was constructed.

[0072] Use the Illumina Hiseq2500 sequencer to perform DNA sequencing and extract high-quality sequenced fragments for subsequent work such as bacterial genome assembly, annotation, and comparative genome analysis.

[0073] 2.2 Genome assembly

[0074] Use Velvet microbial genome assembly software to assem...

Example Embodiment

[0080] Example 3

[0081] This example is a specific example for verifying that the purified colony prepared in Example 1 participates in fat metabolism.

[0082] 3.1 DNA extraction, library construction and sequencing

[0083] Using the mouse low-dose fecal DNA extraction kit (Meiji Biotech, Guangzhou), the bacterial DNA was extracted according to the product specification and technical means well known to those of ordinary skill in the art.

[0084] Using the TruSeq DNA sample preparation kit, according to the product specification and technical means well known to those of ordinary skill in the art, a DNA library with double-end 150bp and insert length 250-300bp was constructed.

[0085] Use the Illumina Hiseq2500 sequencer to perform DNA sequencing and extract high-quality sequenced fragments for subsequent work such as bacterial genome assembly, annotation, and comparative genome analysis.

[0086] 3.2 Genome assembly

[0087] Velvet microbial genome assembly software was used to ass...

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Abstract

The invention belongs to the technical field of microorganisms and particularly relates to a probiotic and a preparation method thereof. The invention provides the probiotic, and the preservation number of the probiotic is GDMCC60085. The invention further provides a preparation method of the probiotic. The preparation method comprises the steps of (1) preparing a bacteria solution; (2) carrying out PCR amplification; and (3) cultivating and purifying a bacterial colony. The invention further provides a probiotic solid yeast, wherein the probiotic solid yeast is prepared from the probiotic. According to the technical scheme, through a whole-genome sequencing experiment, the prepared strain can participate in carbohydrate metabolism and fat metabolism, meanwhile, the vitamin and short-chain fatty acid acetic acid can be produced, and the probiotic is the probiotic with good potential. Meanwhile, the preparation method is simple and easy to operate, and suitable for large-scale popularization and application. The technical problems of a single probiotic variety and complicated separation of the prior art are solved.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a probiotic and a preparation method thereof. Background technique [0002] Probiotics are a class of active microorganisms that are beneficial to the host. They are a general term for active beneficial microorganisms that colonize the human intestinal tract and reproductive system and can produce definite health effects to improve the host's micro-ecological balance and exert beneficial effects. Probiotics play an important role in the health of the host, mainly including the following aspects: 1. Help the host digest and absorb nutrients; 2. Produce important substances such as vitamins, short-chain fatty acids and antioxidants; 3. By inhibiting the growth of harmful bacteria Growth and removal of toxins produced by harmful bacteria to help the host resist bacterial and viral infections and enhance the host's resistance. [0003] At present, the probiotics d...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20C12N1/205C12R2001/01
Inventor 彭永正伍菲凡郭先凤易江丰
Owner GUANGZHOU KANGZE MEDICAL TECH CO LTD
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