Soybean protein composite polymer micro-gel-based radical antioxidant and preparation method thereof

A composite polymer and soybean protein technology, which is applied in the field of soybean protein composite polymer microgel-based antioxidants and its preparation, can solve the problems of limited application, low swelling performance of pure PVA gel, and reactive inertness, and achieve oxygen The effect of improving the ability of free radicals

Active Publication Date: 2017-08-18
NORTHWEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, pure PVA gel has low swelling performance and inert reaction, thes

Method used

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  • Soybean protein composite polymer micro-gel-based radical antioxidant and preparation method thereof
  • Soybean protein composite polymer micro-gel-based radical antioxidant and preparation method thereof
  • Soybean protein composite polymer micro-gel-based radical antioxidant and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Preparation of soybean protein isolate dispersion: take 0.01 g of soybean protein isolate, disperse it in 10 mL of 0.5 mol / L NaOH aqueous solution, and prepare a soybean protein isolate dispersion with a concentration of 1 mg / mL;

[0035] (2) Preparation of soybean protein isolate composite polymer microgel: Heat the above soybean protein isolate dispersion to 50°C under stirring, and add 60 mL of 0.5 mg / mL polyethylene dropwise with a syringe at a constant speed under high-speed (600 rpm) stirring Alcohol solution (containing 0.03 g of polyvinyl alcohol), continued to stir for 1 h after dripping, frozen at –20 °C for 24 h, thawed at room temperature for 24 h, and dialyzed in distilled water for 24 h with a 2000 Da dialysis bag (replace 3 ~5 dialysate), to obtain soybean protein isolate composite polymer microgel;

[0036](3) Preparation of soybean protein composite polymer microgel-based antioxidant: Add the above soybean protein isolate composite polymer microgel ...

Embodiment 2

[0039] (1) Preparation of soybean protein isolate dispersion: take 0.025 g of soybean protein isolate, disperse it in 10 mL of 1mol / L NaOH aqueous solution, and prepare a soybean protein isolate dispersion with a concentration of 2.5 mg / mL;

[0040] (2) Preparation of soybean protein isolate composite polymer microgel: Heat the above soybean protein isolate dispersion to 70°C under stirring, and add 50 mL of 2 mg / mL polyethylene dropwise at a constant speed with a syringe under high-speed (700 rpm) stirring Alcohol solution (containing 0.1 g of polyvinyl alcohol), continued to stir for 5 h after dripping, frozen at –15 °C for 12 h, thawed at room temperature for 36 h, and dialyzed in distilled water for 12 h with a 10,000 Da dialysis bag (replace 3 h during the dialysis process). ~5 dialysate), to obtain soybean protein isolate composite polymer microgel;

[0041] (3) Preparation of soybean protein composite polymer microgel-based antioxidant: add the above soybean protein iso...

Embodiment 3

[0044] (1) Preparation of soybean protein isolate dispersion: take 0.02 g of soybean protein isolate, disperse it in 10 mL of 2.5mol / L KOH aqueous solution, and prepare a soybean protein isolate dispersion with a concentration of 2 mg / mL;

[0045] (2) Preparation of soybean protein isolate composite polymer microgel: Heat the above soybean protein isolate dispersion to 90°C under stirring, and add 20 mL of 6 mg / mL polyethylene dropwise at a constant speed with a syringe under high-speed (800 rpm) stirring Alcohol solution (containing 0.12 g of polyvinyl alcohol), continued to stir for 2.5 h after dripping, frozen at –10°C for 36 h, thawed at room temperature for 12 h, and dialyzed in distilled water for 36 h with a 18,000 Da dialysis bag (need to be replaced during the dialysis process for 3~ 10 times of dialysate) to obtain soybean protein isolate composite polymer microgel;

[0046] (3) Preparation of soybean protein composite polymer microgel-based antioxidant: Add the abov...

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Abstract

The invention provides a soybean protein composite polymer micro-gel-based antioxidant. Soybean protein isolate with poor hydrophilicity and relative stability is combined with water-soluble polymer polyvinyl alcohol, a hydrogen bonding effect on hydroxyl, carboxyl and amino contained in the soybean protein isolate and hydroxyl contained in polyvinyl alcohol is achieved through a heat dispersion and cold shrinkage method, so that soybean protein polymer micro-gel which can be uniformly dispersed in water is formed; then the micro-gel is combined with a salicylaldehyde-cysteine schiff base metal coordination compound to obtain the soybean protein composite polymer micro-gel-based antioxidant. A research on the anti-oxidization performance proves that the composite polymer micro-gel-based antioxidant has strong ability of removing oxygen free radicals; therefore, the composite polymer micro-gel-based antioxidant can be used as super oxide dismutase (SOD) analogue, and has good application prospect in the fields of medicines, food processing and packaging materials.

Description

technical field [0001] The invention relates to a soybean protein composite polymer microgel-based antioxidant and a preparation method thereof, which can be used as an SOD enzyme mimic and belongs to the fields of natural polymer composite materials and biomedical materials. Background technique [0002] Active oxygen, including superoxide anion radicals, hydroxyl radicals, hydrogen peroxide and singlet oxygen, is a natural by-product of human metabolism. But when it is in excess, it attacks biomolecules such as lipids, proteins, enzymes, DNA and RNA, causing cell or tissue damage. Superoxide dismutase (SOD) is a metalloenzyme in vivo, which can catalyze the disproportionation reaction of oxygen free radicals to balance oxygen free radicals in the body. Although natural SOD has a strong ability to scavenge oxygen free radicals, its extraction process is complicated, the price is high, and the permeability through the cell membrane is poor. Therefore, research on SOD enzym...

Claims

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Application Information

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IPC IPC(8): A61K9/06A61K47/42A61K47/32A61K31/555A61K31/30A61P39/06C08J3/075C08L29/04C08L89/00
CPCA61K9/06A61K31/30A61K31/555A61K47/32A61K47/42C08J3/075C08J2329/04C08J2489/00
Inventor 何玉凤闫桂芳钱立忠王荣民宋鹏飞
Owner NORTHWEST NORMAL UNIVERSITY
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