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Lactobacillus pentosus with effects of lowering cholesterol and lowering triglyceride and application

A Lactobacillus pentosus and cholesterol-lowering technology, applied to Lactobacillus, bacteria used in food preparation, applications, etc., can solve the problem of low degradation rate, achieve good bile salts, good safety, lower cholesterol and lower triglycerides. Ester powerful effect

Active Publication Date: 2017-08-18
NORTHWEST UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN 104818230A Lactobacillus plantarum (L01), CN 102899276A Streptococcus thermophilus (BLST), CN102604851A Lactococcus lactis, CN101559082A Lactobacillus (MA2), CN1186855A Urinary Streptococcus (DM891128) and CN 104789488A Lactobacillus rhamnosus (SCF 18 2012) It has a certain effect in the effect of lowering cholesterol, but the degradation rate is not high, and the patent of Lactobacillus pentosus in lowering cholesterol has not been reported, and the strains of lactic acid bacteria in lowering triglycerides are rarely reported.

Method used

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  • Lactobacillus pentosus with effects of lowering cholesterol and lowering triglyceride and application
  • Lactobacillus pentosus with effects of lowering cholesterol and lowering triglyceride and application
  • Lactobacillus pentosus with effects of lowering cholesterol and lowering triglyceride and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Screening and identification of Lactobacillus pentosus EAM-ZL016T with the ability to lower cholesterol and triglyceride provided by the present invention.

[0029] 1 Separation and purification of strains

[0030] Weigh 0.5g of the intestinal content of Tibetan Mastiff in Maqu County, Gansu Province, add it to a test tube filled with 4.5ml sterile water, and dilute it to 10 -6 , take 10 -4 、10 -5 、10 -6 0.1ml each of the three tubes of dilution was applied to the MRS medium for separation by the coating method, and incubated at a constant temperature of 37°C for 48h. Colonies were picked, streaked continuously, separated and purified, and finally a single colony was selected. After microscopic examination confirmed that it was a pure species, it was inoculated into slant medium and cultured for 24 hours, and then stored in a refrigerator at 4°C for later use.

[0031] The formula and conditions of MRS solid medium are: peptone 10g, beef extract 10g, yeast powder 5g...

Embodiment 2

[0042] The cholesterol-lowering effect of Lactobacillus pentosus EAM-ZL016T with the ability of lowering cholesterol and triglyceride provided by the present invention.

[0043] 1 Effect of lowering cholesterol bacteria in egg yolk

[0044]Under aseptic conditions, a certain amount of egg yolk is added to the sterilized culture medium as a cholesterol source, and aseptically evenly dispensed into test tubes. With 2% (thalline concentration is 10 8 cfu / ml) was added to the bacterial solution, incubated at 37°C for 24 hours, centrifuged at 8000r / min for 10 minutes, and the supernatant was taken to determine the cholesterol content in the supernatant according to the phosphorus-sulfur-iron colorimetric method. Two repetitions were set for each group of experiments.

[0045] Configuration of ferric sulfur phosphorus color reagent - ferric chloride solution: weigh 5.0g FeCl 3 ·6H 2 O was dissolved in 200ml of concentrated phosphoric acid; phosphorus sulfur iron reagent (P-S-Fe ...

Embodiment 3

[0061] The triglyceride-lowering effect of Lactobacillus pentosus EAM-ZL016T with the ability to lower cholesterol and triglyceride provided by the present invention.

[0062] Under aseptic conditions, add a certain amount of standard triglycerides to the sterilized medium, and aseptically dispense it into test tubes. With 2% (thalline concentration is 10 8 cfu / ml) was added to the bacterial solution (the uninoculated test tube was used as a blank control), incubated at a constant temperature of 37°C for 24 hours, and the triglyceride degradation rate was measured at 420nm.

[0063] Triglyceride reduction rate=(C-A) / C×100%

[0064] In the formula, A is the OD value at 420nm of the culture solution after the fermentation of the strain, and C is the OD value at 420nm of the blank control.

[0065] The triglyceride-lowering ability of the bacterial strain EAM-ZL016T was determined to be 2.8% through experiments.

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Abstract

The invention discloses lactobacillus pentosus (La.Pentosus), separated and purified from intestinal tracts of Tibetan mastiff in Maqu county, Gansu province, with effects of lowering cholesterol and lowering triglyceride, wherein the preservation number is CGMCC 7426. An experiment proves that the strain has very high acid-resistant ability and bile salt-resistant ability, the cholesterol (TC) can be significantly lowered and the triglyceride (TG) can be lowered. An In-vivo experiment in mice finds that the strain is free of any toxic or side effect, so that the strain can be used for preparation of health drugs and functional foods capable of reducing lipid and lowering cholesterol.

Description

[0001] 【Technical field】 [0002] The invention relates to a lactobacillus pentosus, in particular to a lactobacillus pentosus (La. Pentosus) with the effect of lowering cholesterol and triglyceride and application thereof. [0003] 【Background technique】 [0004] Cholesterol is an essential nutrient for the human body and bears important physiological functions in the body. However, the content of cholesterol in human serum is often too high, and a series of cardiovascular diseases caused by high serum cholesterol are common. Modern research has found that atherosclerosis, venous thrombosis and hypercholesterolemia are closely related. WTO has predicted that by 2030, cardiovascular disease will be the main cause of death, affecting the health of about 236 million people around the world. It has been reported that the incidence of heart disease in Western and Eastern European countries is due to excess blood cholesterol. Compared with people with normal blood lipid levels, pe...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P3/06A23L33/135C12R1/225
CPCA23V2002/00A61K2035/115C12R2001/225C12N1/205A23V2400/167A23V2200/3262
Inventor 吴慧昊牛荇洲牛锋
Owner NORTHWEST UNIVERSITY FOR NATIONALITIES
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