A Quantitative Analysis Method for Microbial Pollution Sources of Water Body

Abstract

The invention discloses a quantitative analysis method for a microbial contamination source of a water body. The method comprises steps as follows: specific biomarker primers which are high in specificity and sensitivity for a target area and capable of being quantitatively analyzed are selected, and a standard curve equation is constructed with a qPCR (quantitative polymerase chain reaction) amplification technology; an actual water area sample is collected, and genomic DNA (deoxyribonucleic acid) of the water sample is extracted; qPCR amplification is performed by adopting regional specific primers, a result is substituted into the standard curve equation for calculation, a concentration value of each microorganism in the target area is obtained, and the contamination source of the target water area is analyzed according to the concentration value of each microorganism. The method can be used for identifying low-concentration target microorganisms, and is suitable for environmental water bodies with different water quality, thereby providing reliable basis for monitoring, source tracing and treatment of the environmental water bodies.

Description

technical field [0001] The invention generally relates to environmental monitoring technology, in particular to a method for quantitatively analyzing sources of microbial pollution in water bodies. Background technique [0002] Untreated (or incompletely treated) human and livestock manure pollution is the main cause of microbial pollution in water bodies. How to quickly and accurately distinguish the main sources of microbial pollution in water bodies and carry out targeted source control in a timely and effective manner is the key to solving the problem link. At present, only about 30% of drinking water sources meet the standards in my country, and the main type is biological pollution (69.1%), which is much higher than the proportion of chemical pollution. [0003] Countries around the world usually use fecal coliforms or Escherichia coli as indicator microorganisms to reflect the microbial pollution of water bodies, but these traditional indicators can only reflect the ...

AI Technical Summary

Problems solved by technology

However, the interaction between various intestinal flora in the host's digestive tract is extremely complex, and the differences in the external environment can easily lead to changes in the genes of the intestinal flora in the digestive tract, resulting in differences in the expression of specific genes in different regions. The specificity of primers in different regions may vary greatly, and the evolution process of the environment cannot be fully represented by a single gene, so the research on biomarkers of regional adaptation should be actively carried out

So far, there has been no research on the quantitative identification of human, cow, pig, chicken and other animal feces pollution in water bodies using fluorescent quantitative PCR technology (qPCR), and there is no related patented technology

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