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Method for evaluating quality of FRUCTUSSCHISANDRAE SPHENANTHERAE in commercially available Chinese patent medicines by using flash-gas chromatography

A technology of Schisandra chinensis and gas chromatography, which is applied in the field of evaluating the quality of Schisandra chinensis, a commercially available Chinese patent medicine, can solve the problems that there are no literature reports on the quality evaluation method of Schisandra chinensis, and achieve the effects of saving experimental time, simplifying experimental steps, and easy operation

Active Publication Date: 2017-08-18
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Existing literature on the detection methods of lignans in the raw medicinal materials of Schisandra chinensis and Chinese patent medicines containing Schisandra chinensis, commonly used methods include thin-layer chromatography, liquid chromatography, gas chromatography, etc., but soaking extraction, ultrasonic extraction, Microwave-assisted extraction and other cumbersome pretreatment methods, and there is no literature report on the quality evaluation method of Zhongnan Schisandra, a Chinese patent medicine containing Schisandra chinensis

Method used

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  • Method for evaluating quality of FRUCTUSSCHISANDRAE SPHENANTHERAE in commercially available Chinese patent medicines by using flash-gas chromatography
  • Method for evaluating quality of FRUCTUSSCHISANDRAE SPHENANTHERAE in commercially available Chinese patent medicines by using flash-gas chromatography
  • Method for evaluating quality of FRUCTUSSCHISANDRAE SPHENANTHERAE in commercially available Chinese patent medicines by using flash-gas chromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Qualitative analysis of lignans in the raw medicinal materials of Schisandra chinensis

[0029] (1) Instruments and reagents

[0030] American ThermoFinnigan Trace DSQ gas chromatography / mass spectrometer (GC-MS); American ThermoFinnigan Trace GC Ultra gas chromatograph with hydrogen flame detector (FID); Japanese Frontier PY-3030D double-click vertical micro-furnace cracker.

[0031] Purchase locally produced schisandra from Shaanxi, Sichuan, Shanxi, and pharmacies, remove visible branches, sawdust and other sundries, bake at 60°C for 5 hours, take out and quickly grind through a 120-mesh sieve to make schisandra powder for later use. See Table 1 for the list of samples.

[0032] Table 1: List of raw medicinal materials of Schisandra chinensis

[0033]

[0034] (2) Experimental method

[0035] Accurately weigh 0.3 mg of Schisandra chinensis (S1) powder produced in Shaanxi, put it into a sample cup, fix it on the injection rod, and put it into the cracke...

Embodiment 2

[0043] The investigation of embodiment 2 reproducibility

[0044] American Thermo Trace GC ultra gas chromatograph (UA-5 metal capillary column, 30m×0.25mmi.d.×0.25μm, film thickness 5% methylpolysiloxane, Japan), Japan Frontier PY-3030D double-click vertical type Micro Furnace Cracker.

[0045] Gas phase conditions: heating program: initial temperature 50°C, rise to 200°C at a rate of 10°C / min, then rise to 300°C at a rate of 10°C / min, and keep for 10 minutes; the temperature of the injection port is 300°C, and the temperature of the detector is 300°C ℃, split ratio 30:1; carrier gas nitrogen or helium, flow rate 1.0mL / min.

[0046] Cracker conditions: cracking furnace temperature: 300°C; interface temperature between cracker and gas chromatograph: 300°C.

[0047] Accurately weigh 3 parts of 0.3 mg of Southern Schisandra powder prepared by the method in Example 1 and put it into a sample cup, fix it on the injection rod, and put it into the cracker installed above the GC in...

Embodiment 3

[0049] Example 3 Quantitative analysis of lignans in the raw medicinal materials of Schisandra chinensis

[0050] The gas chromatograph of ThermoFinnigan Trace in the United States and the Japan Frontier PY-2020iD double-click vertical micro-furnace cracker.

[0051] The gas phase conditions are: the chromatographic column is a UA-5 metal capillary column (30m×0.25mmi.d.×0.25μm, film thickness 5% methyl polysiloxane, Japan). Raise to 200°C at a rate of ℃ / min, then rise to 300°C at a rate of 10°C / min, and hold for 10 minutes; the temperature of the injection port is 300°C, the temperature of the detector is 300°C, and the split ratio is 30:1; the carrier gas is nitrogen or Helium, flow rate 1.0mL / min.

[0052] Cracker conditions: cracking furnace temperature: 300°C; interface temperature between cracker and gas chromatograph: 300°C.

[0053] Make 8 lignan standard products into 20-10000 mg / L methanol solution (mixed standard), draw 1 microliter of the solution and inject it i...

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Abstract

The invention relates to a method for evaluating quality of FRUCTUSSCHISANDRAE SPHENANTHERAE in commercially available Chinese patent medicines by using a flash-gas chromatography. The method comprises the steps of putting a powdered or liquid Chinese patent medicine sample, which contains the FRUCTUSSCHISANDRAE SPHENANTHERAE, into a pyrolyzer, and placing the pyrolyzer at a sample inlet of a gas chromatograph; when the temperature of the pyrolyzer reaches a certain value, carrying out sample introduction; detecting the sample by using the gas chromatograph to obtain a gas chromatogram; carrying out qualitative analysis on a lignan component in the Chinese patent medicine sample by using standard material retention time, and determining the corresponding peak positions of anwuligan, deoxyschizandrin and schisantherin in the gas chromatogram; (2) then, using the flash-gas chromatography to carry out quantitative analysis on the deoxyschizandrin and the schisantherin in the Chinese patent medicine sample by means of an external standard method; if the quantitative result shows that the relative contents of the deoxyschizandrin and the schisantherin in the Chinese patent medicine sample are within a range of 1.50-2.0, proving that the quality of the FRUCTUSSCHISANDRAE SPHENANTHERAE in the Chinese patent medicine sample is better, and otherwise, proving that the quality of the fructus schisandrae in the Chinese patent medicine sample is poorer. The method has the characteristics of being simple, environmentally friendly and efficient.

Description

[0001] (1) Technical field [0002] The invention relates to a method for evaluating the quality of the commercially available Chinese patent medicine Schisandra chinensis. [0003] (2) Background technology [0004] Schisandra chinensis is the dry and mature fruit of Schisandra esphenanthera Rehd.et Wils., a perennial epiphytic herb of the Schisandra family Magnoliaceae (Magnoliacae), mainly produced in central areas such as Shaanxi, Sichuan and Shanxi. Schisandra chinensis mainly contains various chemical components such as lignans, volatile oils, crude polysaccharides and fatty acids, and has high medicinal value. Traditional medicine believes that it has the effects of astringing the lungs and promoting body fluid, nourishing the kidneys and nourishing the heart, and astringing and astringent. It is generally believed that the biological activity of Schisandra chinensis mainly comes from lignans, especially lignans with biphenylcyclooctene as the mother nucleus. Due to its...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 王丽丽黄翌磊黄忠平
Owner ZHEJIANG UNIV OF TECH
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