A method for induction and differentiation of leaf callus of saline-alkali-tolerant fast-growing Ulmus ulma clone

A technology of callus induction and callus, which is applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of high cost, resources, waste, and low differentiation rate of callus adventitious buds, and achieve It is suitable for industrial production, the effect of increasing the differentiation rate and the callus induction rate

Active Publication Date: 2018-11-09
山东建筑大学风景园林规划研究所 +1
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The callus induction rate of the above-mentioned medium is high (89%-100%), but the overall callus adventitious bud differentiation rate is low (50%-76%), resulting in high cost and waste of resources in industrialized production of Ulmus mellifera

Method used

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  • A method for induction and differentiation of leaf callus of saline-alkali-tolerant fast-growing Ulmus ulma clone
  • A method for induction and differentiation of leaf callus of saline-alkali-tolerant fast-growing Ulmus ulma clone
  • A method for induction and differentiation of leaf callus of saline-alkali-tolerant fast-growing Ulmus ulma clone

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Experimental program
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Effect test

Embodiment 1

[0027] (1) Selection of explants: callus-induced explants of saline-alkali-tolerant fast-growing Ulmus spp. clones originate from rooted seedlings cultured in the factory of Ulmus elm, that is, to select robust growth, stretched leaves, tissue-cultured seedlings with plant height > 2.5cm, Rooted seedling leaves (including petioles) or stems with uniform growth were used as callus induction materials.

[0028] (2) Explant treatment: according to figure 1 (Left) Cut the area shown, and cut the robust stem segment between the second leaf and the fifth leaf from top to bottom of the tissue cultured seedling. When cutting, the parts with axillary bud nodes are removed, and only the internode parts are taken as test materials, and the cutting length is about 0.3-0.4 cm. After cutting, insert the stem section into the medium in time, place the stem section horizontally on the surface of the medium, inoculate 5 stem section materials in each bottle (diameter 6.5cm; height 9.5cm) medi...

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Abstract

The invention discloses a callus induction and differentiation method for clonal leaves of salt-alkali-resistant fast-growing ulmus pumila. The method adopts leaves of a tissue seedling as a callus induction material, adopts an MS culture medium (1,4-D 0.5mg / L) to perform the callus induction, and adopts an EM culture medium (6-BA 0.3 mg / L) to perform the subculture and promote the formation of adventitious buds. By adopting the formula and method of the invention, and a callus forming rate of the ulmus pumila leaves is 100 percent; the final differentiation rate of the callus adventitious buds is 95 percent; and the final differentiation rate of the callus adventitious buds is increased by 20 to 45 percent compared with the preliminary research result.

Description

technical field [0001] The invention relates to a method for plant callus induction, in particular to a method for inducing and differentiating callus of the saline-alkali-tolerant fast-growing Ulmus ulma clone. Background technique [0002] White elm (Ulmus pumila L.), a plant of the genus Ulmus in the family Ulmaceae, has many varieties. As one of the main ornamental tree species in gardens in my country, white elm is highly resistant to toxic gases such as smoke, sulfur dioxide and hydrogen fluoride, and has extremely high environmental adaptability, anti-pollution, resistance to diseases and insect pests, suitable for reproduction and strong salt tolerance Alkaline properties make Ulmus elm very promising to become the backbone tree species for landscaping in saline-alkaline areas. [0003] The successful experience and cases of woody plants in plant tissue culture production are relatively rare compared with herbaceous plants. The lack of basic medium suitable for the g...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 慕徳宇房用梁玉慕宗昭王强范小莉董举文
Owner 山东建筑大学风景园林规划研究所
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