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Pulse negative-pressure low-temperature lentinan extraction method

A technology of negative pressure and low temperature, lentinan, applied in the field of polysaccharide extraction, can solve the problems of glycosidic bond breaking conformation, long extraction time, high equipment cost, etc., achieve the effect of maintaining physiological activity, improving extraction efficiency, and increasing wall breaking rate

Active Publication Date: 2017-08-22
HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, hot water and dilute alkali solution are mostly used for the extraction of lentinan. The Chinese patent application number 201410700171.5 discloses a semi-bionic method for extracting lentinan. The main steps are: raw material pretreatment, weak acidic water extraction, weak alkalinity Water extraction, neutral water extraction, preparation of eluent, preparation of retentate, concentration under reduced pressure, centrifugation to remove sediment, precipitation vacuum drying and crushing packaging; although this method has a high extraction yield, it has a long extraction time and is likely to cause polysaccharides Glycosidic bond breakage and conformational changes affect its activity
In addition, there are also reports on the extraction of Lentinan by ultrasonic waves and microwaves. Although the extraction time is shortened, the cost of equipment is high, which limits its industrial application.

Method used

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  • Pulse negative-pressure low-temperature lentinan extraction method
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for pulse negative pressure low temperature extraction of lentinan, comprising the following steps:

[0028] (1) Put the cleaned raw materials of fresh shiitake mushrooms and water into the extraction device according to the mass ratio of 1:5, and perform beating by shearing and stirring at a speed of 5000 rpm to obtain shiitake mushroom slurry;

[0029](2) Extract the shiitake mushroom slurry under the condition of shearing and stirring (5000 r / min), evacuate to -95 kPa, keep it for 2 minutes, return to normal pressure, cycle 4 times, and centrifuge to obtain the material slag and supernatant; extract the slag under the condition of shearing and stirring, vacuumize to -95 kPa, keep it for 2 minutes, return to normal pressure, and cycle 3 times in this way, and obtain the filter residue and filtrate after centrifugation , combining the filtrate and the supernatant to obtain the extract;

[0030] (3) Concentrate the extract obtained in step (2) under reduced pr...

Embodiment 2

[0032] A method for pulse negative pressure low temperature extraction of lentinan, comprising the following steps:

[0033] (1) Put the washed raw materials of fresh shiitake mushrooms and water into the extraction device according to the mass ratio of 1:8, heat to 50°C, shear and stir at a speed of 10,000 rpm for beating, and obtain shiitake mushroom slurry;

[0034] (2) Extract the shiitake mushroom slurry under the condition of shearing and stirring (10000 r / min), evacuate to -93kPa, keep it for 2 minutes, return to normal pressure, cycle 5 times, and centrifuge to obtain the slag and supernatant; the residue is extracted under the condition of shearing and stirring, vacuumized to -93 kPa, kept for 2 minutes, then returned to normal pressure, and circulated for 5 times, after centrifugation, filter residue and filtrate are obtained, Combine the filtrate and supernatant to obtain the extract;

[0035] (3) Concentrate the extract obtained in step (2) under reduced pressure ...

Embodiment 3

[0037] A method for pulse negative pressure low temperature extraction of lentinan, comprising the following steps:

[0038] (1) Dry, crush, and pass through a 40-mesh sieve to obtain dried shiitake mushroom powder. Put the dried shiitake mushroom powder and water into the extraction device at a mass ratio of 1:10, heat to 50°C, and use 500 rpm Shear and stir at a rotating speed to obtain a shiitake mushroom slurry;

[0039] (2) Extract the shiitake mushroom slurry under the condition of shearing and stirring (500 r / min), evacuate to -97 kPa, keep it for 1 minute, return to normal pressure, cycle 3 times, and centrifuge to obtain the material slag and supernatant; extract the slag under the condition of shearing and stirring, vacuumize to -97 kPa, keep it for 1 minute, return to normal pressure, and cycle 2 times, and obtain the filter residue and filtrate after centrifugation , combining the filtrate and the supernatant to obtain the extract;

[0040] (3) Concentrate the ex...

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Abstract

The invention discloses a pulse negative-pressure low-temperature lentinan extraction method, which comprises the following steps of adding water into fresh mushroom raw materials; performing pulping to obtain mushroom pulp; or drying the fresh mushroom raw materials; performing crushing to obtain dry mushroom powder; adding water into the dry mushroom powder; performing uniform mixing to obtain mushroom pulp; extracting the mushroom pulp under the shearing stirring conditions at the extraction temperature not higher than 50 DEG C and at the extraction pressure being the pulse negative pressure; after the extraction is completed, performing solid-liquid separation; obtaining filter slag and extraction liquid; concentrating the extraction liquid; performing precipitation by ethyl alcohol; further drying the precipitates; obtaining a finished product. The method provided by the invention has the advantages that the resource waste and the environment pollution are reduced; the added value of the mushroom is increased; the duplex effects of mechanical shearing and the negative pressure cavitation are utilized for promoting the lentinan dissolution; the yield of the lentinan is improved; the cost is low; the environment is protected; the operation is simple; the safety is realized; the energy sources are saved, and the like.

Description

technical field [0001] The invention belongs to the technical field of polysaccharide extraction, and in particular relates to a method for extracting lentinan at low temperature under pulse negative pressure. Background technique [0002] Shiitake mushrooms are delicious, fragrant and nutritious. They not only contain a variety of nutrients such as polysaccharides, cellulose, amino acids, lentinin, mushroom essence and mineral elements such as iron, calcium, phosphorus, etc., but also rich in provitamin D. Shiitake mushrooms have a good immune effect. It can improve the immune function of the body, delay aging, prevent cancer, lower blood pressure, lower blood fat, and lower cholesterol. In addition, shiitake mushrooms also have therapeutic effects on diabetes, tuberculosis, infectious hepatitis, neuritis, etc. , and can be used for indigestion, constipation, etc., and these effects are closely related to its polysaccharides. [0003] However, shiitake mushrooms are food m...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 李顺峰王安建田广瑞魏书信刘丽娜
Owner HENAN ACAD OF AGRI SCI