Electrochemical biosensor targeting PTK7K based on Co-Thi-Sgc8c and preparation method and application thereof
A co-thi-sgc8c, biosensor technology, applied in the field of electrochemical biosensing, can solve the problems of low practical application value, high cost of nano-gold, low success rate and efficiency, etc., to improve the ability to transfer electrons, Effect of short response time and improved efficiency
Active Publication Date: 2017-08-25
NANJING NORMAL UNIVERSITY
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Problems solved by technology
Due to the low success rate and efficiency of layer-by-layer self-assembly, the detection limit is not too low
Moreover, gold nanoparticles are relatively expensive, resulting in high cost and low practical application value.
Method used
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Experimental program
Comparison scheme
Effect test
Embodiment 1
[0030] Preparation of metal thionine coordination compound Co-Thi:
[0031] Take 0.05mmol of thionine and 0.5mmol of sodium ethoxide, add 0.6mmol of cobalt acetate, dissolve in 10ml of ethanol, and react in a reaction kettle at 150°C for 6 days, cool down naturally, and centrifuge and wash the product with ethanol and hot water to obtain metal thionine complex. bit compound Co-Thi.
Embodiment 2
[0033] Preparation of metal thionine coordination compound Co-Thi:
[0034] Take 0.05mmol of thionine and 0.6mmol of sodium ethoxide, add 0.5mmol of cobalt acetate, dissolve in 10ml of ethanol, and react in a reactor at 150°C for 5 days, and cool down naturally. The product is centrifuged and washed with ethanol and hot water to obtain the metal thionine coordination compound Co-Thi.
Embodiment 3
[0036] Example 3 has the same formula and preparation method as Example 1, except that it is reacted at 140° C. for 7 days in a reactor.
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Abstract
The invention discloses an electrochemical biosensor targeting PTK7K based on Co-Thi-Sgc8c and a preparation method and application thereof. The sensor is prepared by coating Co-Thi-Sgc8c suspension on the surface of a glassy carbon electrode in a droplet mode, Co-Thi-Sgc8c is obtained by the synthesis of a metal thionine coordination compound Co-Thi and an aptamer Sgc8c, and the metal thionine coordination compound Co-Thi is prepared from thionine, cobalt acetate and sodium ethoxide with the molar ratio of 1 to (10-12) to (10 12). The electrochemical biosensor is simple in preparation, convenient to use, capable of detecting PTK7K effectively, high in detection sensitivity, short in response time and high in specificity, and has reproducibility and stability.
Description
technical field [0001] The invention belongs to the application field of electrochemical biosensing, and in particular relates to an electrochemical biosensor based on Co-Thi-Sgc8c targeting PTK7K and its preparation method and application. Background technique [0002] Tumors have become a global problem in human health, and protein tyrosine kinase-7 (PTK7K) is a member of defective receptor protein tyrosine kinases overexpressed in a variety of human malignancies. Currently existing detection methods are difficult to qualitatively evaluate and quantify the expression of PTK7K protein in cancer tissues. The Sgc8c aptamer (Apt) is a DNA that can specifically bind protein tyrosine kinase-7 (PTK7K). Thionine (Thi) is a thiophene small molecule compound with a ring-shaped planar structure and a positively charged aromatic ring structure. The molecular structure of thionine can make it easy to insert into DNA molecules, but it can also generate strong electrostatic attraction w...
Claims
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IPC IPC(8): G01N27/327G01N27/48C07F15/06
CPCG01N27/308G01N27/3275G01N27/48C07F15/065
Inventor 周泊潘竞雄
Owner NANJING NORMAL UNIVERSITY