Roseobacter sp. and application thereof
A technology of bacilli and microbial strains, applied in the field of Rhodobacter, can solve problems such as low yield, inability of plants to survive, and difficulty in plant growth, and achieve the effects of promoting germination and growth, promoting plant growth, and improving soil microenvironment
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Embodiment 1
[0009] Example 1 Separation of marine bacteria
[0010] A surface water collector was used to take 53 surface seawater samples from the Cangzhou sea area of Bohai Bay in my country, using 2216E medium (the formula is: peptone 5g, yeast powder 1g, iron phosphate 0.1g, agar powder 15g, seawater 1000ml, and pH adjusted with NaOH For 7.6, use after sterilization at 121°C for 20 minutes), and use the method of diluting and coating to separate marine samples. Cultivate the coated plate in an incubator at 28°C. After the plate grows out of bacteria, pick out single colonies of different shapes and use the streaking method to separate and purify three times to obtain pure culture colonies of marine bacteria, and a total of marine bacteria are obtained 126 plants. After mixing the liquid culture of a single colony with 60% glycerol in a ratio of 1:1, store it in an ultra-low temperature refrigerator at -80°C.
Embodiment 2
[0011] Example 2 Screening of marine growth-promoting bacteria
[0012] The preserved strains isolated from the ocean were activated on the LB plate, and then the activated marine bacteria were inoculated into an Erlenmeyer flask containing 20ml of seawater culture medium at an inoculum of 1%, and cultured at 28°C for 12h. Centrifuge the bacterial solution at 5000 rpm for 10 min, discard the supernatant and add 5 ml of sterile water and shake to suspend the bacterial body. Then centrifuge again at 5000 rpm for 10 min, and discard the supernatant. Resuspend the bacteria in sterile water and adjust the OD 600 0.2-0.3. After soaking cucumber and wheat seeds in the adjusted concentration of bacteria solution for 4 hours, place the seeds neatly on sterilized filter paper and add 5 ml of sterile water. Put it into a plant culture room at 25±2℃ for 3 days, and measure the shoot length and root length regularly. Table 1 shows the promotion results of some strains on cucumber and wheat ...
Embodiment 33-2
[0015] Example 3 3-2 Growth promotion experiment on soil cultured cucumber
[0016] Mix the vermiculite and nutrient soil 1:1 evenly and add it to the square flower pot. Place the filled square flower pots into two large white trays, add 3L of sterile water to one tray, and add 3L of OD to the other tray 600 A bacterial solution of Rhodobacter 3-2 with a value of 0.1. After the soil is completely moistened, at least two cucumber seeds are cultivated in the middle of the square flowerpot and placed in the cultivation room for cultivation. When the cucumber grows a true leaf, leave a cucumber seedling with the same growth in each pot. After 6 weeks of cultivation in the greenhouse, the cucumber plants were taken out and washed, and the plant height, dry weight and wet weight were measured (Table 3). According to Table 2, it can be seen that the plant height, dry weight and wet weight of the treatment with strain 3-2 are higher than the blank control, indicating that the applicati...
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