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A method of processing low-value fish antibacterial peptides

A processing method and antimicrobial peptide technology, which is applied in the field of low-value fish antimicrobial peptide processing, can solve the problems of low purity, residual impurities, and low amount of antimicrobial peptides, and achieve improved hydrolysis activity, broad antibacterial spectrum, and improved hydrolysis reaction speed effect

Inactive Publication Date: 2017-08-29
舟山达康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The Chinese invention patent of CN 103388014 B discloses a preparation method of ferrous chelated antimicrobial peptide. The method has the characteristics of simple operation and low cost, but it has the disadvantages of residual impurities, less antimicrobial peptide acquisition, and low purity.

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  • A method of processing low-value fish antibacterial peptides

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Embodiment 1

[0019] A processing method of low-value fish antimicrobial peptides, including: preparation and sterilization, degreasing, microwave extraction and purification, centrifugal purification, enzymolysis, centrifugal purification, ammonium sulfate purification and finished products, specifically including the following steps:

[0020] 1) Preparation and sterilization: Take some fresh fish bladder, cut open and remove blood vessels and mucous membranes, wash with distilled water 3 times, cut and weigh 10g into a 500ml airtight stainless steel container, add 25g distilled water, at a pressure of 50MPa, Steam for 30 minutes at a temperature of 90℃;

[0021] 2) Degreasing: Add 0.010g of lipase to the fish maw slurry obtained after step 1, stir and ventilate at 35°C to react for 20 minutes, and then heat to 90°C and cook for 5 minutes to deactivate the enzyme. To normal temperature, complete lipase hydrolysis and fatty acid oxidation to obtain defatted fish maw slurry;

[0022] 3) Microwave ...

Embodiment 2

[0029] A processing method of low-value fish antimicrobial peptides, including: preparation and sterilization, degreasing, microwave extraction and purification, centrifugal purification, enzymolysis, centrifugal purification, ammonium sulfate purification and finished products, specifically including the following steps:

[0030] 1) Preparation and sterilization: Take a few fresh swim bladders, cut open and remove the blood vessels and mucous membranes, wash them with distilled water 5 times, cut and weigh 15g into a 500ml sealable stainless steel container, add 30g distilled water, at a pressure of 80MPa, Steam for 50 minutes at 100℃;

[0031] 2) Degreasing: Add 0.015g of lipase to the fish maw slurry obtained after step 1, stir and ventilate at 36°C for 30 minutes to react, then heat to 100°C and cook for 5 minutes to inactivate the enzyme. To normal temperature, complete lipase hydrolysis and fatty acid oxidation to obtain defatted fish maw slurry;

[0032] 3) Microwave extracti...

Embodiment 3

[0039] A processing method of low-value fish antimicrobial peptides, including: preparation and sterilization, degreasing, microwave extraction and purification, centrifugal purification, enzymolysis, centrifugal purification, ammonium sulfate purification and finished products, specifically including the following steps:

[0040] 1) Preparation and sterilization: Take some fresh fish bladder, cut open and remove blood vessels and mucous membranes, wash it with distilled water 5 times, cut and weigh 12g into a 500ml airtight stainless steel container, add 28g distilled water, at a pressure of 70MPa, Cook for 40 minutes at 95℃;

[0041] 2) Degreasing: Add 0.014g of lipase to the fish maw slurry obtained after step 1. Stir and ventilate at 37°C to react for 25 minutes, then heat to 95°C and cook for 5 minutes to inactivate the enzyme. To normal temperature, complete lipase hydrolysis and fatty acid oxidation to obtain defatted fish maw slurry;

[0042] 3) Microwave extraction and puri...

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Abstract

A method of processing low-value fish antibacterial peptides is disclosed. The method includes steps of preparing, sterilizing, degreasing, performing microwave extraction for purification, performing centrifugal purification, performing enzymatic hydrolysis, performing centrifugal purification, and performing purification with ammonium sulfate to obtain a finished product. Swim bladders of low-value fish are degreased, impurities are removed by filtration, proteins in the swim bladders are purified, then the enzymatic hydrolysis is performed to hydrolyze the proteins into antibacterial peptides, and enzymatic-activity peptides are used to increase activity of compound protease, thus accelerating hydrolysis and increasing the processing efficiency. Beneficial effects of the method are that the swim bladders of low-value fish are utilized to produce the antibacterial peptides to achieve high-value utilization of the swim bladders, the antibacterial peptides prepared by the method have characteristics of capability of being natural, nontoxic, pollution-free, antibacterial, efficient, wide in antibacterial spectrum, and the like, and the finally obtained antibacterial peptides are high in yield and purity, can be applied in industrial production and have a good development prospect.

Description

Technical field [0001] The invention relates to the field of antibacterial peptide processing, in particular to a method for processing low-value fish antibacterial peptides. technical background [0002] Antibacterial peptides are a kind of small molecule peptides with antibacterial effect, generally composed of 12-15 amino acids. Antibacterial peptides have reduced the incidence of infectious diseases due to their excellent antibacterial properties, and become an ideal substitute for antibiotics. They have been discovered in nature. There are more than 2,000 kinds of antibacterial peptides. Antibacterial peptides have broad-spectrum antibacterial properties and can kill Gram-positive bacteria, Gram-negative bacteria and fungi. Antibacterial peptides as antibacterial drugs have the advantages of no toxic side effects, no residues, no pollution, etc. At present, the preparation of antibacterial peptides and the study of antibacterial mechanism have been widely carried out. One o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/46
CPCC07K14/461C12P21/06
Inventor 叶力
Owner 舟山达康科技有限公司
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