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Composition for preventing or improving non-alcoholic fatty liver disease containing white lentil extract as an active ingredient

A non-alcoholic, active ingredient technology, applied in the field of health functional food composition and pharmaceutical composition, can solve the problems of non-alcoholic fatty liver efficacy research, increase of physiological activity, reporting, etc.

Active Publication Date: 2021-01-08
KOREA INST OF ORIENTAL MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] White lentils have been used as a folk remedy since ancient times to invigorate the stomach, remove dampness, and relieve heat. Korean Patent No. 0316269 discloses that the methanol extract of white lentil leaves has the effect of increasing physiological activity. Korean Patent No. 0929979 discloses It has been known that the traditional Chinese medicine composition containing white lentils has the effect of reducing liver and kidney toxicity induced by the administration of anticancer agents, but there is no report on the study of the effect on non-alcoholic fatty liver

Method used

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  • Composition for preventing or improving non-alcoholic fatty liver disease containing white lentil extract as an active ingredient
  • Composition for preventing or improving non-alcoholic fatty liver disease containing white lentil extract as an active ingredient
  • Composition for preventing or improving non-alcoholic fatty liver disease containing white lentil extract as an active ingredient

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Experimental program
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Effect test

preparation example Construction

[0065] 2) Preparation of Oil Red O working solution

[0066] Mix 6 ml of the Oil Red O stock solution and 4 ml of ddH 2 After O was mixed, it was filtered through a 0.2 μm filter and left at room temperature for 20 minutes.

[0067] 3) Preparation of 10% formalin solution

[0068] ddH in 63ml 2 A stock solution (37%, Merck, Cat# K36658003) obtained by diluting 27ml of formalin stock solution in O was mixed with 10ml of 10XPBS.

[0069] 4) 100% isopropanol (Isopropanol; Merck, Cat# K36543834) was used as a blank control.

[0070] 5) Preparation of 60% isopropanol

[0071] Mix 6 ml of the 100% isopropanol and 4 ml of ddHO 2 O made by mixing.

Embodiment 1

[0072] Example 1. Liver cancer cell line (HepG 2 ) cultivation

[0073] Hepatoma cell line (HepG 2 ) was purchased from American Type Culture Collection (ATTC) and used. Hepatoma cell line (HepG 2 ) was cultured in a DMEM (Dulbecco's modified Eagle's medium) medium containing 10% FBS and 1% penicillin-streptomycin. Incubate the above cells at 37 °C in 5% CO 2 cultured in an incubator, and the cultured cells were cultured in a certain number (3×10 4 Cells / 500 μl well) were seeded into 24-well plates and experimented.

[0074] After the cells were seeded in the 24-well plate, the cells were completely attached to the 24-well plate and had the shape of the cells, and were used when the cells reached 75% confluence.

Embodiment 2

[0075] Embodiment 2. Preparation of free fatty acid (Free Fatty Acid; FFA)

[0076] (1) Preparation of 100mM palmitate (Sigma P-0500) and 100mM oleate (Sigma O-75010) stock solutions

[0077] It was prepared at 70° C. using a 0.1 M NaOH solution as a solvent, filtered through a 0.2 μm filter, and then sterilized.

[0078] (2) Preparation of 5% (w / v) BSA (Sigma A-6003) solution not containing free fatty acids

[0079] distilled water (ddH 2 O) It was prepared for use as a solvent, sterilized, and refrigerated at 4°C.

[0080] (3) The concentration of oleic acid ester and palmitic acid ester is the preparation of the 5mM mixed fatty acid stock solution that mixes at 2:1

[0081] At 60°C, the 5% (w / v) BSA solution that did not contain free fatty acids dissolved in distilled water prepared in (2) above was used as a solvent to prepare 10 ml of a fatty acid stock solution, so that the above (1) The concentrations of the respective fatty acids prepared in 2.0 were up to 5 mM. F...

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Abstract

A health functional food composition and a pharmaceutical composition for preventing or ameliorating a non-alcoholic fatty liver disease include an extract of Dolichos lablab Linne as an active ingredient. The extract of Dolichos lablab Linne, being an active ingredient, effectively reduces accumulation of triglyceride in hepatocarcinoma cell line (HepG2). Therefore, the compositions are used for functional foods and medicines for the prevention or amelioration of non-alcoholic fatty liver.

Description

technical field [0001] The invention relates to a health functional food composition and a pharmaceutical composition for preventing or improving non-alcoholic fatty liver disease, which contain white lentil (Dolichos lablab Linne) extract as an active ingredient. Background technique [0002] Liver disease mortality rate in South Korea is very high at 23.5 per 100,000 population, ranking first among 40-year-olds (41.1 per 100,000) and second among 50-year-olds (72.4 per 100,000), accounting for the third cause of death in the 30-year-old group (10 per 100,000), and liver disease is the main cause of death for the middle-aged population in South Korea. [0003] Among the liver diseases, fatty liver refers to a phenomenon exhibited by abnormal deposition of neutral fat that does not exist in normal cells in hepatocytes. About 5% of the normal liver is composed of adipose tissue, neutral fat, fatty acid, phospholipid, cholesterol and cholesteryl ester are the main components ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L33/105A23K10/30A61K36/48A61P1/16A61P3/04A61P3/10
CPCA23V2002/00A61K36/48A23K10/30A23L33/105A23V2200/02A23V2200/10A23V2200/222A23V2200/32A23V2200/332A23V2200/328A23V2250/21A23V2250/61A23V2250/5114A23V2250/5118A23V2250/6406A23V2250/612A23V2250/628A23V2250/5022A23V2250/6418A23V2250/642A23V2250/5026A23V2250/5432A23V2250/51084A23V2250/5108A23V2250/51088A23V2250/1578A23V2250/082A23V2250/1628A23V2250/161A61K2236/331A61P1/16A61P3/04A61P3/06A61P3/10
Inventor 宋光薰金润希李惠园林阿郎
Owner KOREA INST OF ORIENTAL MEDICINE
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