Preparation method of multifunctional mimic enzyme composite sphere and application thereof

A technology of simulating enzymes and composite balls, applied in chemical instruments and methods, water pollutants, water/sludge/sewage treatment, etc., can solve the problems of weakened catalytic activity, easily destroyed structure, low catalytic activity, etc., and achieve recycling Easy and fast operation, low cost and high catalytic activity

Inactive Publication Date: 2017-09-08
QUFU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, heme itself has low catalytic activity and is insoluble in water. It is easy to aggregate into dimers with low activity in aqueous solution, and its structure is easily destroyed in oxidative media, resulting in weakened catalytic activity.

Method used

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  • Preparation method of multifunctional mimic enzyme composite sphere and application thereof
  • Preparation method of multifunctional mimic enzyme composite sphere and application thereof
  • Preparation method of multifunctional mimic enzyme composite sphere and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A preparation method of a multifunctional imitation enzyme composite ball, the proportioning ratio of each raw material is as follows:

[0041] HAuCl 4 : 10.0 mM, 1.0 mL

[0042] Hemin: 0.25 mg mL -1 , 1.0 mL

[0043] NaOH: 1.0 M, 105 μL

[0044] SG: 5.0mg

[0045] SA: 0.5 wt%, 1.0 mL

[0046] Fe 3 o 4 : 15.0 mg

[0047] CaCl 2 : 1.0 wt %, 20.0 mL

[0048] The steps are:

[0049] 1) At room temperature, the FeCl 3 ·6H 2 O (1.73 g ) was dissolved in ethylene glycol (35 mL) with a concentration of 0.183M, stirred by magnetic force, and after it was completely dissolved, 3.83 g of sodium acetate was added, the sodium acetate was 0.804 M in the solution, continued to stir for 30 min and then transferred to high pressure In the still, adjust the temperature to 200 °C, react for 8 h, then cool to room temperature, separate with a magnet, collect the solid adsorbed by the magnet, wash with water and ethanol, and dry at 60 °C for 12 h to obtain hollow nanospheres F...

Embodiment 2

[0053] A preparation method of a multifunctional imitation enzyme composite ball, the proportioning ratio of each raw material is as follows:

[0054] HAuCl 4 : 10 mM, 1.0 mL

[0055] Hemin: 0.60 mg mL -1 , 1.0 mL

[0056] NaOH: 1.0 M, 115 μL

[0057] SG: 10.0mg

[0058] SA: 1.0 wt%, 1.0 mL

[0059] Fe 3 o 4 : 25 mg

[0060] CaCl 2 : 1.5 wt%, 20 mL

[0061] The steps are:

[0062] 1) At room temperature, the FeCl 3 ·6H 2 O (1.73 g ) was dissolved in ethylene glycol (35 mL) with a concentration of 0.183M, stirred by magnetic force, and after it was completely dissolved, 3.83 g of sodium acetate was added, the sodium acetate was 0.804 M in the solution, continued to stir for 30 min and then transferred to high pressure In the still, adjust the temperature to 200 °C, react for 8 h, then cool to room temperature, separate with a magnet, collect the solid adsorbed by the magnet, wash with water and ethanol, and dry at 60 °C for 12 h to obtain hollow nanospheres Fe 3 o...

Embodiment 3

[0066] A preparation method of a multifunctional imitation enzyme composite ball, the proportioning ratio of each raw material is as follows:

[0067] HAuCl 4 : 10.0 mM, 1.0 mL

[0068] Hemin: 1.0 mg mL -1 , 1.0 mL

[0069] NaOH: 1.0 M, 125 μL

[0070] SG: 12.0mg

[0071] SA: 2.5w%, 1.0mL

[0072] Fe 3 o 4 : 35.0 mg

[0073] CaCl 2 : 3.0w%, 20mL

[0074] The steps are:

[0075] 1) At room temperature, the FeCl 3 ·6H 2 O (1.73 g ) was dissolved in ethylene glycol (35 mL) with a concentration of 0.183M, stirred by magnetic force, and after it was completely dissolved, 3.83 g of sodium acetate was added, the sodium acetate was 0.804 M in the solution, continued to stir for 30 min and then transferred to high pressure In the still, adjust the temperature to 200 °C, react for 8 h, then cool to room temperature, separate with a magnet, collect the solid adsorbed by the magnet, wash with water and ethanol, and dry at 60 °C for 12 h to obtain hollow nanospheres Fe 3 o 4...

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Abstract

The invention relates to a preparation method of a multifunctional mimic enzyme composite sphere and application thereof. A one pot type in-situ synthesis method is adopted; in an alkaline condition, hemin is used as a reducing agent and a stabilizing agent; after the hemin is mixed with chloroauric acid (HAuCl4), a mineralization reaction is carried out; a gold nanocluster mimic enzyme (Hemin-Au) with high catalytic activity is formed; afterwards, the gold nanocluster mimic enzyme, spherical graphene (SG) and ferroferric oxide (Fe3O4) are mixed and react; subsequently, sodium alginate (SA) and a calcium ion are added to carry out embedment treatment, so as to obtain the multifunctional mimic enzyme composite sphere (SA-Hemin-Au-SG-Fe3O4). The preparation method does to relate to the use of a complicated photoelectric instrument, and has advantages of being high in the catalytic activity, simple to operate, quick in response and low in cost, and the like. The mimic enzyme composite sphere is favorable in effect when being used for the catalysis, the adsorption and the degradation of some environmental organic poisons, such as phenol (Ph), methylene blue (MB), rhodamine B (RB) and the like.

Description

technical field [0001] The invention belongs to the technical field of enzyme catalysis and catalytic degradation of environmental poisons, and relates to a preparation method and application of a multifunctional simulated enzyme composite ball. Background technique [0002] Enzymes are important biocatalysts with excellent properties such as strong specificity, high catalytic efficiency, and no pollution. The vast majority of enzymes are macromolecular proteins, which must be catalyzed under mild and suitable conditions. They are unstable in high temperature, high ion concentration, strong acid, strong alkali and some organic solvents, and are easy to denature and inactivate to reduce their catalytic performance. . In order to solve these problems, researchers try to modify natural enzymes to form mimetic enzymes to improve their catalytic performance. Mimetic enzymes are a class of synthetic non-protein structure catalysts that have similar catalytic activity to natural ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F101/30C02F101/34
CPCC02F3/342C02F2101/308C02F2101/345
Inventor 王桦张立燕董敏敏陈丽君刘欢段志强
Owner QUFU NORMAL UNIV
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