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Glycosylated hemoglobin, hemoglobin detection kit and detection method thereof

A technology of glycosylated hemoglobin and detection kit, which is applied in the field of glycosylated hemoglobin detection, can solve the problems such as unfavorable development of convenient household detection devices, unfavorable promotion of convenient kits, complicated technical solutions, etc., and achieves the goal of being favorable for market promotion and increasing detection accuracy. The effect of reducing the amount of blood collection

Active Publication Date: 2019-03-08
深圳市东邦生物医疗技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although this technical solution can measure the content of glycosylated hemoglobin more accurately than the existing technology to a certain extent, its technical solution is complex and takes a long time to detect, which is not conducive to the development of portable household detection devices
The applicant also retrieved that the invention patent with application publication number CN105301261A discloses a kit for detecting glycosylated hemoglobin and its preparation method and use method, wherein the kit includes a reaction solution, a washing solution and a reaction plate, but the reaction The storage conditions of liquid and washing liquid are strictly limited to 2-8°C, which limits the detection under natural conditions and is not conducive to the promotion of convenient kits

Method used

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  • Glycosylated hemoglobin, hemoglobin detection kit and detection method thereof
  • Glycosylated hemoglobin, hemoglobin detection kit and detection method thereof
  • Glycosylated hemoglobin, hemoglobin detection kit and detection method thereof

Examples

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Embodiment 1

[0035] Embodiment 1 provides a kind of glycosylated hemoglobin and hemoglobin detection kit, including R1 reagent, R2 reagent, R3 reagent, R4 reagent, glycosylated hemoglobin-hemoglobin deposition film; wherein,

[0036] R1 reagent: its pH is 7.2-7.5, the solvent is an aqueous solution of sodium chloride with a concentration equal to 0.35wt%, the solute and its content in the solvent are: surfactant 0.12% w / v; the surfactant specifically refers to The mass ratio of Tween20 and Triton X-100 is 2:1;

[0037] R2 reagent: the solvent is glycine-sodium hydroxide buffer solution, the solute and its content in the solvent are: xylene-aniline-phenylboronic acid conjugate 0.001wt%, surfactant 0.12%w / v; The surfactant specifically refers to Tween20 and Triton X-100 with a mass ratio of 2:1;

[0038] R3 reagent: specifically physiological saline;

[0039]R4 reagent: the solvent is glycine-sodium hydroxide buffer solution, the solute and its content in the solvent are: zinc chloride 10m...

Embodiment 2

[0042] Embodiment 2 provides a kind of glycosylated hemoglobin and hemoglobin detection kit, including R1 reagent, R2 reagent, R3 reagent, R4 reagent, glycosylated hemoglobin-hemoglobin deposition film; wherein,

[0043] R1 reagent: its pH is 7.2-7.5, the solvent is a sodium chloride aqueous solution with a concentration of 0.20wt%, the solute and its content in the solvent are: surfactant 0.18% w / v; the surfactant specifically refers to The mass ratio of Tween20 and Triton X-100 is 2:1;

[0044] R2 reagent: the solvent is glycine-sodium hydroxide buffer solution, the solute and its content in the solvent are: xylene-aniline-phenylboronic acid conjugate 0.003wt%, surfactant 0.18%w / v; The surfactant specifically refers to Tween20 and Triton X-100 with a mass ratio of 2:1;

[0045] R3 reagent: specifically glycine-sodium hydroxide buffer;

[0046] R4 reagent: the solvent is glycine-sodium hydroxide buffer solution, the solute and its content in the solvent are: zinc sulfate 15...

Embodiment 3

[0049] Embodiment 3 provides a method for detecting glycosylated hemoglobin and hemoglobin, specifically based on the kits provided in Embodiment 1 and Embodiment 2, and specifically includes the following steps:

[0050] Take 2.5 μL of the blood sample to be tested and 100 μL of R1 reagent, shake for 3 seconds, and obtain the blood sample after hemolysis;

[0051] Add an equal volume of R2 reagent to the hemolyzed blood sample and shake for 3 seconds to obtain a test sample for glycosylated hemoglobin;

[0052] Put the glycosylated hemoglobin test sample on the glycosylated hemoglobin-hemoglobin deposition film, let it stand for 5s, then add the R4 reagent solution, and let it stand for 5s;

[0053] Washing the glycosylated hemoglobin-hemoglobin deposition film with R3 reagent;

[0054] The sample sites on the glycosylated hemoglobin-hemoglobin deposition film are detected based on reflectance photometry, and the contents of glycosylated hemoglobin and hemoglobin in the bloo...

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Abstract

The invention belongs to the technical field of glycated hemoglobin detection, discloses a glycated hemoglobin and hemoglobin detection kit and a detection method based thereon, and particularly relates to a glycated hemoglobin and hemoglobin detection kit based on boric acid affinity chromatography and a detection method based thereon; the kit comprises reagent R1, reagent R2, reagent R3, reagent R4, and glycated hemoglobin-hemoglobin deposited film; the detection method is specifically a method of detecting glycated hemoglobin and hemoglobin at the same time. Compared with the prior art, the glycated hemoglobin and hemoglobin detection kit and the detection method based thereon have the advantages that by using the kit and detection method, it is possible to shorten the detection time, lower blood taking amount, extend shelf life of the kit, improve detection accuracy, and improve mildness of detection conditions.

Description

technical field [0001] The invention belongs to the technical field of glycosylated hemoglobin detection, and in particular relates to glycosylated hemoglobin, a hemoglobin detection kit and a detection method thereof. Background technique [0002] According to IDF statistics, there were about 415 million people with diabetes worldwide in 2015, and 1 in every 11 people has diabetes. It is predicted that by 2040, there will be 642 million people with diabetes in the world, 151 million people with diabetes in China, and as many as 224 million people with prediabetes. According to statistics from the National Health and Family Planning Commission and IDF, the prevalence of diabetes among adults aged 18 and over in my country was 10.7% in 2015, and the prevalence of prediabetes was as high as 16%. . [0003] Diabetic patients in China rank first in the world and are tending to be younger. According to DF statistics, 1.3 million people died of diabetes and its complications in m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/72
CPCG01N33/723G01N2333/805
Inventor 杨晓斌伯彬李淮彬张耀祥
Owner 深圳市东邦生物医疗技术有限公司