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Method for culturing chlorella by using crude glycerine optimized sludge dewatering liquid

A technology for sludge dehydration and crude glycerin, which is applied in the field of environmental engineering and microalgae biology, can solve the problems of difficult treatment and high cost of crude glycerin purification, and achieve the effect of reducing costs

Active Publication Date: 2017-09-19
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the purification cost of crude glycerin is high and difficult to handle

Method used

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  • Method for culturing chlorella by using crude glycerine optimized sludge dewatering liquid
  • Method for culturing chlorella by using crude glycerine optimized sludge dewatering liquid
  • Method for culturing chlorella by using crude glycerine optimized sludge dewatering liquid

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Experimental program
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Effect test

Embodiment 1

[0030] Embodiment 1: Sludge dehydration liquid treatment

[0031] The settled and filtered sludge dehydration liquid was divided into two parts: one part was used as the original water sample (without any treatment), and the other part was sterilized at 121°C for 30 minutes as the sterilized water sample for the experiment . The water quality indicators before and after sterilization are shown in Table 1.

[0032] Table 1 Water quality characteristics before and after sterilization

[0033]

Embodiment 2

[0034] Example 2: Chlorella growth under different concentrations of crude glycerol in non-sterilized sludge dehydration liquid

[0035] Centrifuge the chlorella liquid cultivated to the logarithmic phase at 4000r / min for 5 minutes, wash with deionized water three times and then centrifuge, and dehydrate the unsterilized sludge with crude glycerin concentration of 0, 1, 2, 4, 6g / L respectively Resuspend the algal bodies (numbers 0, 1, 2, 4, 6) in 600ml of liquid solution, adjust the algae density (OD) to about 0.3, and place the Erlenmeyer flask in a constant temperature light incubator. The culture conditions were 25°C, 5000 lux light, 12h:12h light-to-dark ratio, and the pH of each sewage water sample was not adjusted. Shake the Erlenmeyer flask 5 times a day and take samples regularly.

[0036] Determination of algae density (OD680nm) in the water sample, the experimental results are as follows figure 1 As shown, the biomass of Chlorella increased with the culture time un...

Embodiment 3

[0037] Example 3: Nitrogen and phosphorus consumption under different crude glycerol concentrations in unsterilized sludge dehydration liquid

[0038] The water sample sample of timing sampling among the embodiment 2 measures the change of nutrient salt (N, P) content in the water sample, and experimental result is as follows figure 2 , image 3 , Figure 4 . Such as figure 2 As shown, the ammonia nitrogen content decreased with the culture time. The removal rates of ammonia nitrogen in the non-sterilized experimental group were 64%, 98%, 99%, 95% and 91%, respectively. The ammonia nitrogen content of the crude glycerin low concentration experimental group decreased rapidly in the first two days, and was basically fully utilized on the third day, with a good removal effect.

[0039] Such as image 3 As shown, the total nitrogen content decreased continuously with the culture time. The final total nitrogen content of the experimental group without crude glycerin was hi...

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Abstract

The invention discloses a method for culturing chlorella by using crude glycerine optimized sludge dewatering liquid, and belongs to the technical field of environment engineering and microalgae biology. Crude glycerine with different concentrations is used as a carbon source for chlorella growth; the crude glycerine is added into the sludge dewatering liquid for improving the biomass of chlorella. The method has the advantages that biodiesel byproducts of crude glycerine are used as the carbon source to be added into the sludge dewatering liquid for culturing the chlorella; during the biomass energy source production, nitrogen and phosphorus in the sewage are recovered and utilized; the sewage treatment cost is reduced. The win-win effect of environment benefits and economic benefits is realized; meanwhile, the chlorella absorbs and utilizes the crude glycerine; the treatment cost of the crude glycerine is reduced; in the culture process, other nitrogen, phosphorus and carbon sources are not added, so that the considerable biomass is realized.

Description

technical field [0001] The invention relates to a method for cultivating chlorella by using crude glycerin to optimize sludge dehydration liquid, and belongs to the field of environmental engineering and microalgae biotechnology. Background technique [0002] Microalgae have the characteristics of high photosynthetic efficiency, short growth cycle, no need to occupy agricultural land, and strong environmental adaptability. Microalgae are easy to optimize and control to increase yield, easy to crush and dry, and can produce high-value by-products such as protein and pigment. Among all biodiesel feedstocks, microalgae have the highest oil production rate and fix carbon dioxide during the growth and accumulation of oil. [0003] With the acceleration of urbanization in my country, the discharge of urban domestic wastewater has also increased. Although the method of using microorganisms to treat wastewater can convert nitrogen in wastewater into nitrogen gas for discharge, pho...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 任洪艳脱金华阮文权
Owner JIANGNAN UNIV
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