Detection method for clenbuterol residues in animal hairs

Abstract

The invention discloses a detection method for clenbuterol residues in animal hairs. The detection method comprises the following steps: cleaning a cleaning solution for the animal hairs, cutting the animal hairs into pieces (preferably, the length is not greater than 2 cm), and drying the animal hairs; putting the dried hairs into a weak alkaline solution, performing stirring at the pressure of 10 to 15 atm for 1 to 2.5 hours, then relieving the pressure, adding an organic solvent, performing ultrasonic treatment for 10 to 20 minutes, standing the solution for layering, and extracting supernate serving as an extracting solution; purifying the extracting solution through a WCX or MCX column; measuring the content of the clenbuterol residues in the extracting solution through ultra-high performance liquid chromatography-electrospray tandem mass spectrometry. According to the detection method, the animal hairs can be cleaned, the extraction effect is good, the interference with the detection process is little, and the detection accuracy is high; occurrence of false positive detection is greatly avoided; the clenbuterol recycling rate is high.

Description

technical field [0001] The invention relates to a detection of veterinary drug residues, in particular to a detection method of clenbuterol residues in animal hair. Background technique [0002] Clenbuterol, also known as clenbuterol hydrochloride, is a drug that can promote the growth of lean meat and inhibit the growth of fat meat. A large number of experiments have proved that the residues of clenbuterol hydrochloride in animals are mainly concentrated in the eyes, hair, Lung, liver, kidney and muscle and adipose tissue. Among them, the retina, choroid, and hair of the eye have the highest residues because they are eliminated the slowest; the next are lungs, liver, and kidneys; the residues in muscle tissue and adipose tissue are roughly the same, about 1 / 5 of those in the liver. The urine or muscle tissue of animals is often used in the detection of clenbuterol in the technology. Such raw materials are likely to cause false positives during detection, and the detection ...

AI Technical Summary

Problems solved by technology

Among them, the retina, choroid, and hair of the eye have the highest residues because they are eliminated the slowest; the next are lungs, liver, and kidneys; the residues in muscle tissue and adipose tissue are roughly the same, about 1 / 5 of those in the liver. In the technology, animal urine or muscle tissue is often used in the detection of clenbuterol. Such raw materials are likely to cause false positives and the detection accuracy is not high.

There are many residues in the hair, and the possibility of false positive judgment will be greatly reduced, but because the hair is seriously dirty, the extraction is difficult, and the recovery rate of clenbuterol is easy to reduce, and the detection accuracy needs to be improved