High density fermentation method of genetically engineered bacterium of recombinant interferon

Abstract

The invention provides a high density fermentation method of a genetically engineered bacterium of recombinant interferon, and belongs to the field of fermentation engineering. The high density fermentation method comprises following steps: a genetic engineering seed stored in liquid nitrogen after construction recombination is subjected to activation, shaking culturing, and amplification culturing, and is delivered into a fermentation cylinder for aeration stirring culturing, when bacterium OD600 is increased to be about 5, an inducing agent is added; ingredient supply, stirring speed, and aeration amount are adjusted based on a bacterial strain growth curve, nutrient requirements, and pH change in the fermentation cylinder; and discharging is carried out when conversion from growth period into decline phase is realized, and crude purification and condensation are carried out, so that a crude enzyme product is obtained. According to the high density fermentation method, optimization of dissolved oxygen amount, fermentation medium, medium supply, and supplying method is realized, so that nutrient conditions and external environment required by rapid growth and metabolisms of bacteria are ensured, bacterium content and product yield in fermentation cylinder discharging are increased greatly, and bacterium content is increased from 25g / L to 45g / L.

Description

technical field [0001] The invention belongs to the technical field of fermentation process, and mainly relates to a high-density fermentation method of recombinant interferon genetically engineered bacteria. Background technique [0002] Interferon (Interferon, IFN) was first discovered by the British scientist Isaacs in 1957 when he used chicken embryo chorioallantoic membrane to study the interference phenomenon of influenza virus. It is a cytokine that has the functions of inhibiting cell division, regulating immunity, anti-virus, Anti-tumor and other effects. Its essence is protein, and its types can be divided into α, β, γ, ω and so on. IFN can induce cells to develop resistance to viral infection. It prevents or limits viral infection by interfering with viral gene transcription or translation of viral protein components. It is currently the most important anti-viral infection and anti-tumor biological product. [0003] Interferon is a trendy new drug. According to...

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Problems solved by technology

Relatively speaking, the production of IFN induced by inducers such as Chinese herbal medicine has greatly limited its clinical and scientific application due to the complex purification process, low yield, and mild effect.

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