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Method for detecting trichlorphon through quantum dot-labeled bionic immunoassay-capillary electrophoresis

A technology of capillary electrophoresis and bionic immunity, which is applied in the rapid and sensitive detection of trichlorfon, quantum dot label bionic immunoassay-capillary electrophoresis detection of trichlorfon, can solve the problems of low sensitivity, long detection time, expensive instruments, etc. Achieve high sensitivity, low detection limit, and reduce the effect of detection limit

Active Publication Date: 2017-09-26
SHANDONG AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to overcome the shortcomings of the traditional trichlorfon detection method, such as long detection time, expensive equipment, low sensitivity, etc., to provide a bionic immunoassay with quantum dot labeling-capillary electrophoresis method for detecting trichlorfon

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  • Method for detecting trichlorphon through quantum dot-labeled bionic immunoassay-capillary electrophoresis
  • Method for detecting trichlorphon through quantum dot-labeled bionic immunoassay-capillary electrophoresis
  • Method for detecting trichlorphon through quantum dot-labeled bionic immunoassay-capillary electrophoresis

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Embodiment Construction

[0020] Below in conjunction with embodiment, the present invention is further described; Following embodiment is illustrative, not limiting, can not limit protection scope of the present invention with following embodiment.

[0021] The invention combines the bionic immune technology with the capillary electrophoresis detection method to establish a rapid detection method with high sensitivity for trichlorfon. Its specific embodiment is:

[0022] 1. Synthesis of quantum dot-labeled hapten: Mix 16 μL trichlorfon hapten (1 mM), 32 μL 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (10 mM) and 10 μL aminoquantum Add points (8μM) to the reaction vessel in turn, and mix well; add 72μL of borate buffer solution with pH 7.4, and react at room temperature in the dark for 2h; The quantum dot-labeled hapten was obtained by filtration and concentration for separation and purification; the quantum dot-labeled hapten was stored at 2-8°C.

[0023] 2. Dissolve 0.257g trichlorfon and 0.172g ...

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Abstract

The invention relates to a method for detecting trichlorphon through quantum dot-labeled bionic immunoassay-capillary electrophoresis. The method comprises the following steps: taking a molecular imprinted polymer as a bionic antibody and a quantum dot as a label; diluting quantum dot-labeled haptens 300-1,000 times with borate solution with the pH value of 7.4, mixing 1-5m: of diluted quantum dot-labeled haptens and 2-5mL of trichlorphon standard sample gradient diluent, adding 5-10mg of molecular imprinted polymer particles into the mixture and stirring the mixture at room temperature for 60-100min; centrifuging the mixture at 5,000r / min for 20min, filtering with a filtration membrane with the thickness of 0.22mum and detecting the trichlorphon through capillary electrophoresis; calculating an inhibition ratio according to the peak area of capillary electrophoresis and drawing a trichlorphon standard curve; replacing the trichlorphon standard sample gradient diluent with sample extracting solution, repeating the operation in step 1) and calculating the content of the trichlorphon in a to-be-detected object. The structure of a quantum dot of the label used in the method is smaller, so that a competing reaction can be promoted; therefore, the sensitivity of the detection method is improved.

Description

technical field [0001] The invention relates to a bionic immunoassay-capillary electrophoresis detection method for trichlorfon with quantum dot labeling, which belongs to the technical field of food safety detection, in particular to a rapid and sensitive detection method for trichlorfon. Background technique [0002] Trichlorfon is widely used in the prevention and control of plant diseases and insect pests, and it can easily enter the human body through the skin and respiratory tract. In recent years, trichlorfon has been widely concerned by countries all over the world because of its high toxicity and potential carcinogenic properties. GB 16319-1996 stipulates that the maximum residue of trichlorfon in food shall not be higher than 0.1mg / kg. [0003] At present, there are many reports on the detection of trichlorfon content in food at home and abroad, including: gas chromatography; high performance liquid chromatography; liquid, gas chromatography and mass spectrometry;...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/561G01N33/58G01N33/532
CPCG01N33/532G01N33/561G01N33/588
Inventor 徐志祥姜名荻孔非凡乔旭光
Owner SHANDONG AGRICULTURAL UNIVERSITY
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