Phosphate solubilizing bacterium rhizosphere fungus and application thereof to promotion of growth of blueberries

A technology of phosphate-solubilizing bacteria and fungi, applied in the field of microorganisms

Active Publication Date: 2017-09-29
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In contrast, there are fewer reports on phosphate-solubilizing fungi

Method used

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  • Phosphate solubilizing bacterium rhizosphere fungus and application thereof to promotion of growth of blueberries
  • Phosphate solubilizing bacterium rhizosphere fungus and application thereof to promotion of growth of blueberries
  • Phosphate solubilizing bacterium rhizosphere fungus and application thereof to promotion of growth of blueberries

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Isolation, purification and screening of phosphorus-solubilizing endophytic fungi from blueberry roots, the steps are as follows:

[0025] 1. Collect wild blueberry roots with soil samples in the Greater Khingan Mountains area, wash the blueberry root sections with clear water, and then transfer them to an ultra-clean workbench for the following operations: soak the root sections in 10% (mass ratio) hydrogen peroxide for 8 minutes, During this period, use sterile tweezers to gently turn it several times to ensure thorough disinfection; after rinsing with sterile water for 2-3 times, transfer it to 6% (mass ratio) sodium hypochlorite, soak for 15 minutes, and rinse with sterile water twice; Blot off any remaining liquid on the root surface. The sterilized root segments were cut into 0.5 cm long root segments with sterile scissors, inserted into the prepared PDA plate, and cultured upside down in the dark in a constant temperature incubator at 25-30°C. Recipe for PDA pla...

Embodiment 2

[0029] Phosphorus dissolving ability test of WR1 strain, the steps are as follows:

[0030] 1. To detect the degradation of inorganic phosphorus and organic phosphorus by strain WR1. After the above primary screening, a strain was obtained, named WR1, and its d / D was 1.02. On the ultra-clean workbench, use a puncher to inoculate 5mm of WR1 bacteria blocks into the Erlenmeyer flasks containing 100mL of inorganic phosphorus and organic phosphorus liquid medium. All treatments were replicated 3 times with the inoculated sterile PDA medium block as the control. After culturing for 7 days, the hyphae were filtered out, and the OD value in the supernatant was measured by the molybdenum antimony anti-colorimetric method, and the available phosphorus content and phosphorus solubilization rate in the supernatant were calculated. Among them, phosphorus dissolving rate (%)=(inoculation soluble phosphorus content-control soluble phosphorus content) / amount of phosphate added×100. There ...

Embodiment 3

[0033] Morphological and molecular biological identification of strain WR1, the steps are as follows:

[0034] 1. Pick the above-mentioned preserved purified strains, inoculate them on PDA plates, and cultivate and activate them in a constant temperature incubator at 28°C for 1-2 weeks. Use a sterile puncher with a diameter of 0.5 cm to take the bacteria cake from the outermost layer of the colony, inoculate it on a new PDA plate, and culture it in a constant temperature incubator at 28°C for 1 week to observe the shape of the colony. Figure 5 It shows that the colony is off-white, fluffy, with dense hyphae, the middle of the colony is raised, and there are radial cracks, and the back of the colony is dark yellow.

[0035] 2. Use an inoculation needle to pick a small amount of purified single-colony hyphae, put it on a glass slide with a drop of normal saline to make a temporary mount, and observe the hyphae structure of the endophytic fungus under a microscope. Image 6 It...

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Abstract

The invention provides phosphate solubilizing bacterium rhizosphere fungus and application thereof to promotion of growth of blueberries and belongs to the technical field of biology. A strain is WR1 and has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.13884; when the strain is inoculated back to a blueberry tissue culture seedling and a blueberry seedling, the strain has a remarkable growth promotion effect. Therefore, the bacterium rhizosphere fungus WR1 can provide a good strain resource for development of microorganism bacterium fertilizer and has a good application and development prospect.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a phosphate-solubilizing mycorrhizal fungus and its application in promoting the growth of blueberries. Background technique [0002] Blueberry, also known as lingonberry, is a plant of the genus Vaccinium in the Rhododendronaceae family. It is an emerging fruit tree with high economic value and broad development prospects. Due to its unique flavor and nutritional and health value, blueberry is known as "the third-generation fruit and one of the five health foods for human beings". In recent years, large-scale cultivation and production have begun in Shandong, Jiangsu, Liaoning and other places in my country, and it has become one of the most promising fruit tree species. With the expansion of the cultivation area, the demand for blueberry seedlings is also increasing. Blueberry, like other rhododendrons, is a shallow-rooted plant without a large tap root. The root sys...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P21/00C12R1/645
CPCA01N63/30C12N1/14C12N1/145C12R2001/645
Inventor 王磊宿红艳李媛媛黄朝陈张胜男孙志超
Owner LUDONG UNIVERSITY
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