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Polymer carbon dot with high fluorescent quantum yield and preparation method thereof, and application of polymer carbon dot to targeted detection of tumor cells

A fluorescent quantum yield and polymer technology, applied in fluorescence/phosphorescence, chemical instruments and methods, measuring devices, etc., can solve the problem of low fluorescent quantum yield of polymer carbon dots, and achieve excellent fluorescent properties and stable fluorescent performance , Excellent stability effect

Active Publication Date: 2017-10-03
JILIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the fluorescence quantum yield of polymer carbon dots prepared is not high. We designed a method to improve the fluorescence quantum yield of polymer carbon dots through intramolecular energy resonance transfer, and selected biological targeting molecule folic acid as raw material to prepare fluorescent quantum yields. polymer carbon dots

Method used

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  • Polymer carbon dot with high fluorescent quantum yield and preparation method thereof, and application of polymer carbon dot to targeted detection of tumor cells
  • Polymer carbon dot with high fluorescent quantum yield and preparation method thereof, and application of polymer carbon dot to targeted detection of tumor cells
  • Polymer carbon dot with high fluorescent quantum yield and preparation method thereof, and application of polymer carbon dot to targeted detection of tumor cells

Examples

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Effect test

Embodiment 1

[0024] (1) In a reactor containing 10 mL of deionized water, add 0.01 mmol of lysine and 0.01 mmol of folic acid, a material that has a targeting effect on cancer cells, and stir vigorously (400 rpm) at room temperature to make the two Fully dissolved to form a yellow aqueous solution;

[0025] (2) After putting the yellow solution obtained in step (1) into a microwave chemical reactor for 10 minutes, the solution changed from light yellow to dark yellow, and obtained polymer carbon dots;

[0026] (3) Filter the liquid obtained in step (2) with a 0.22 μm water phase filter head to remove impurities with larger sizes.

[0027] (4) Subsequently, the solution in step (3) was dialyzed with a dialysis bag with a molecular weight of 800-1000 for 48 hours, and the deionized water was changed every 4 hours. 3mL, measure the fluorescence quantum yield of the product to 56%, put the product in a 4°C refrigerator for later use;

[0028] (5) Dilute the polymer carbon dots obtained in st...

Embodiment 2

[0030] (1) In a reactor containing 10 mL of deionized water, add 0.02 mmol of lysine and 0.01 mmol of folic acid, a material that has a targeting effect on cancer cells, and stir vigorously (400 rpm) at room temperature to make the two Fully dissolved to form a yellow aqueous solution;

[0031] (2) Transfer the yellow solution obtained in step (1) to the lining of a 50mL polytetrafluoroethylene hydrothermal reaction kettle and install a steel jacket, gradually heat it to 180°C in an oven and keep the temperature constant for 10h, and naturally cool to room temperature to obtain Polymer carbon dot aqueous solution;

[0032] (3) Filter the liquid obtained in step (2) with a 0.22 μm water phase filter head to remove impurities with larger sizes.

[0033] (4) Subsequently, the solution in step (3) was dialyzed with a dialysis bag with a molecular weight of 800-1000 for 48 hours, and the deionized water was changed every 4 hours. 3mL, measure the fluorescence quantum yield of the...

Embodiment 3

[0036] (1) In a reactor filled with 12 mL of deionized water, add 0.01 mmol of tyrosine and 0.01 mmol of folic acid, a material that has a targeting effect on cancer cells, mix well at room temperature, and ultrasonicate for 10 minutes to fully dissolve the two , at which point a yellow aqueous solution was formed.

[0037](2) Transfer the yellow solution obtained in step (1) to the lining of a 50mL polytetrafluoroethylene hydrothermal reaction kettle and install a steel jacket, gradually heat to 180°C in an oven and keep the temperature constant for 10h, and naturally cool to room temperature to obtain Polymer carbon dots.

[0038] (3) Filter the liquid obtained in step (2) with a 0.22 μm water phase filter head to remove impurities with larger sizes.

[0039] (4) Subsequently, the solution in step (3) was dialyzed with a dialysis bag with a molecular weight of 800-1000 for 48 hours, and the deionized water was changed every 4 hours. 3mL, measure the fluorescence quantum yi...

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Abstract

The invention provides a polymer carbon dot with high fluorescent quantum yield and a preparation method thereof, and application of the polymer carbon dot to targeted detection of tumor cells, belonging to the technical field of polymer carbon points. According to the invention, a bio-targeting molecule folic acid is used as a raw material and a simple hydrothermal method is employed for one-step synthesis of the polymer carbon spot with high fluorescence quantum yield; and the polymer carbon spot has the functions of targeted detection and selective imaging of tumor cells. The polymer carbon dot provided by the invention has the advantages of simple synthesis method, small and uniform particle size, high quantum yield, low biological toxicity, good biocompatibility and the like. The fluorescent polymer carbon dot has high quantum yield, can be used for biological imaging, presents a tumor cell-targeting function, is applicable as a fluorescent imaging material for diagnosis and treatment of cancers, and plays an important role in fields like tumor detection and drug sustained release.

Description

technical field [0001] The invention belongs to the technical field of polymer carbon dots, and in particular relates to a polymer carbon dot with high fluorescence quantum yield, a preparation method and its application in the detection of targeted tumor cells. Background technique [0002] Polymer carbon dots are a new type of material developed from carbon dots. They are obtained by cross-linking or polymerization of linear polymers or monomers, or composed of polymer chains connected by carbon cores and surfaces. They can be divided into: There are two types of conjugated polymer carbon dots (CPDs) and non-conjugated polymer carbon dots (NCPDs). We synthesized CPDs from conjugated molecules through polymerization, such as hydrothermal, polymerization, cross-linking, self-assembly, and other methods. [0003] At present, nanosensors based on fluorescent materials have attracted widespread attention because of their fast response speed and high spatial resolution. Commonl...

Claims

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Application Information

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IPC IPC(8): C09K11/65C09K11/02G01N21/64
CPCC09K11/025C09K11/65G01N21/6486
Inventor 林权刘厚杨雪张川赵月杨柏
Owner JILIN UNIV
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