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A method for establishing a tea tree tissue culture rapid propagation system

A technique of tissue culture rapid propagation and method establishment, which is applied in the field of establishment of tea tree tissue culture rapid propagation system, can solve the problems of explant browning, difficult rooting, long culture period, etc., achieve genetic stability, short growth cycle, The effect of rapid reproduction

Inactive Publication Date: 2019-11-01
NORTHWEST A & F UNIV
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Problems solved by technology

[0003] In summary, the existing problems in the prior art are: the tea tree itself carries many endophytic bacteria and has a high polyphenol content, so problems such as explant browning and pollution will occur during the test; Difficult, low survival rate in field cultivation

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[0029] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0030] The establishment method of the tea tree tissue culture rapid propagation system provided by the present invention comprises the following steps of explant selection, during the period of 3-5 months, it is collected from the year-old semi-woody shoots with full axillary buds in the greenhouse;

[0031] Primary culture: The best medium for the primary culture of Shaancha No. 1 is MS+6-BA 2.0mg / L+NAA 0.1 mg / L+GA 3 3.0mg / L+PVP 4.0mg / L; the best medium for ‘Anji Yellow Tea’ is MS+6-BA 2.0 mg / L+IBA 0.1mg / L+GA 3 3.0mg / L+PVP 4.0mg / L;

[0032] Subculture: The best medium for subculture of 'Shaancha No. 1' is MS+IBA 0.1mg / ...

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Abstract

The invention belongs to the technical field of tea tree propagation, and discloses an establishment method of a tea tree tissue culture rapid propagation system. The establishment method includes the steps: taking tea tree branches sheared at room temperature, removing large leaves, clipping nodes with axillary buds and cleaning surfaces by cleanser essence; immersing the nodes in alcohol in a sterile room, soaking the nodes in sodium hypochlorite solution and flushing the nodes by sterile water; disinfecting the nodes in the sterile room; illuminating the nodes for 13 hours per day with the light intensity of 1000Lux at the temperature of 25 DEG C and performing primary culture; performing secondary culture; performing root induction; transplanting test tube seedlings and hardening the seedlings. Explants are proliferated according to set series and can be annually produced in a stable culture environment, industrialized production and regulated and programmed production according to a certain technological process can be formed, and the establishment method has the advantages of high propagation speed, tidiness, uniformity, no insects, fewer diseases, short growth cycle and stable heredity.

Description

technical field [0001] The invention belongs to the technical field of tea tree propagation, in particular to a method for establishing a tea tree tissue culture rapid propagation system. Background technique [0002] Tea tree is a perennial woody plant. Due to its long growth cycle, low self-compatibility, and short flowering period, conventional tea tree breeding takes 20-25 years. The seedling cycle is long, the reproduction coefficient is small, and the germplasm is easily degraded. . [0003] In summary, the existing problems in the prior art are: the tea tree itself carries many endophytes and has a high polyphenol content, so problems such as explant browning and pollution will occur during the test; It is difficult, and the survival rate of field cultivation is low. Contents of the invention [0004] Aiming at the problems in the prior art, the invention provides a method for establishing a tea tree tissue culture rapid propagation system. [0005] The present i...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01H5/00
CPCA01H4/001A01H4/008A01H5/00
Inventor 鲍露谷星肖斌郭莎莎高岳芳
Owner NORTHWEST A & F UNIV
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