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A kind of oral gel containing modified papain and its preparation method

A technology of papain and gel, which is applied in the medical field, can solve problems such as redness, blisters, skin allergies, and dyspnea, and achieve the effects of improving antibacterial ability, reducing hygroscopicity, and extending the protection period

Active Publication Date: 2018-08-14
武汉伢典生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the current preparations for removing dental caries all contain chloramine-T ingredients, which will contact the skin during use, and have greater harm to the human body after percutaneous absorption, such as: skin allergies, redness, Blister, easy to produce symptoms such as asthma and dyspnea after being inhaled into the respiratory tract, which will have a serious impact on the human body

Method used

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  • A kind of oral gel containing modified papain and its preparation method
  • A kind of oral gel containing modified papain and its preparation method
  • A kind of oral gel containing modified papain and its preparation method

Examples

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Effect test

Embodiment 1

[0032] Embodiment 1, a kind of oral gel containing modified papain

[0033] The oral gel containing modified papain consists of the following components and their weight percentages:

[0034] Modified papain 4%, citric acid 12%, carbomer 940 4% and distilled water 80%;

[0035] The preparation method of described modified papain is:

[0036] S1 Take papain, add diamide solution with a concentration of 90 μmol / L, the solid-liquid ratio of the above-mentioned papain to diamide solution is 1mg: 8ml; let it stand for 10min to obtain modified papain;

[0037] S2 Add the modified papain obtained in step S1 to a Tris-hydrochloric acid buffer solution with a concentration of 0.02mol / L and a pH value of 7. The solid-to-liquid ratio of the modified papain and Tris-hydrochloric acid buffer solution is 1mg: 1ml, at room temperature Add sodium chloride to the saturated state while stirring to precipitate the free enzyme, let it stand for 25 minutes, centrifuge, take the precipitate, and ...

Embodiment 2

[0041]Embodiment 2, a kind of oral gel containing modified papain

[0042] The oral gel containing modified papain consists of the following components and their weight percentages:

[0043] Modified papain 8%, citric acid 10%, carbomer 940 2% and distilled water 80%;

[0044] The preparation method of described modified papain is:

[0045] S1 Take papain, add diamide solution with a concentration of 95 μmol / L, the solid-liquid ratio of the above-mentioned papain to diamide solution is 1mg: 10ml; let it stand for 12min to obtain modified papain;

[0046] S2 Add the modified papain obtained in step S1 to a Tris-hydrochloric acid buffer solution with a concentration of 0.04mol / L and a pH value of 7.5. Add sodium chloride to the saturated state while stirring to precipitate the free enzyme, let it stand for 30 minutes, centrifuge, take the precipitate, and obtain the modified papain polymer;

[0047] S3 Take the modified papain polymer obtained in step S2 and add a polyethylen...

Embodiment 3

[0049] Embodiment 3, a kind of oral gel containing modified papain

[0050] The oral gel containing modified papain consists of the following components and their weight percentages:

[0051] Modified Papain 14%, Citric Acid 8%, Carbomer 940 1% and Distilled Water 77%;

[0052] The preparation method of described modified papain is:

[0053] S1 Take papain, add diamide solution with a concentration of 100 μmol / L, the solid-to-liquid ratio of papain to diamide solution is 1mg:12ml; let it stand for 15 minutes to obtain modified papain;

[0054] S2 Add the modified papain obtained in step S1 to a Tris-hydrochloric acid buffer solution with a concentration of 0.06mol / L and a pH value of 8. The solid-liquid ratio of the modified papain and Tris-hydrochloric acid buffer solution is 1mg:2ml, Add sodium chloride to a saturated state while stirring to precipitate the free enzyme. After standing still for 35 minutes, centrifuge and take the precipitate to obtain the modified papain p...

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Abstract

The invention belongs to the technical field of medical treatment, and particularly relates to oral gel with modified papain and a preparation method thereof. The oral gel mainly comprises the modified papain, citric acid, carbomer and distilled water. Components of the oral gel are reasonably and scientifically matched and mutually acted, so that the oral gel has the remarkable functions of killing germs, removing dental caries, whitening teeth, treating small oral ulcer and the like and remarkable treatment effect on the dental caries, mechanical property of dentine cannot be affected in the using process of the oral gel, and the oral gel is ideal and has high safety.

Description

technical field [0001] The invention belongs to the field of medical technology, and in particular relates to an oral gel containing modified papain and a preparation method thereof. Background technique [0002] Dental caries is listed by the World Health Organization (WHO) as one of the three major chronic non-communicable diseases that endanger human health, and it is also a common and frequently-occurring disease in children. Bacterial infection is the main pathogenesis of pulp disease and periapical disease, among which Gram anaerobic bacteria are the main pathogenic bacteria, and the detection rate is as high as 98.33%. At present, the treatment of dental caries is mainly completed through several steps such as rot removal, sterilization, and filling. Among them, there are two methods of caries removal and chemical mechanical rot removal. The reagents used in chemical mechanical methods are mainly composed of sodium hypochlorite solution and amino acid gel, which are ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/66A61K8/365A61K8/81A61K8/04A61Q11/00A61P1/02C12N9/96C12N9/50
CPCA61K8/042A61K8/365A61K8/66A61K8/8147A61Q11/00C12N9/63C12N9/96C12Y304/22002
Inventor 岳力群黄安振
Owner 武汉伢典生物科技有限公司