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Preparation method of stem cell preparation for repairing diabetes pancreas islet function

A technology of stem cell preparation and diabetes, which is applied in the field of stem cell regenerative medicine, can solve the problems of difficulty in exerting curative effect and weak targeting, and achieve the effect of no immune rejection, strong targeting and high proliferation characteristics

Inactive Publication Date: 2017-10-17
HEBEI MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, direct transplantation of bone marrow mesenchymal stem cells is often not very targeted, and it is difficult to exert curative effect

Method used

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  • Preparation method of stem cell preparation for repairing diabetes pancreas islet function
  • Preparation method of stem cell preparation for repairing diabetes pancreas islet function
  • Preparation method of stem cell preparation for repairing diabetes pancreas islet function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0020] S1. Acquisition and identification of bone marrow mesenchymal stem cells

[0021] Take out the femur of SD rats, rinse with PBS; cut off the proximal femoral epiphysis, use a syringe to absorb FBS-MSCM medium, insert the needle from the distal epiphyseal cavity, and repeatedly rinse the bone marrow cavity until the femur becomes pale; blow the bone marrow fluid gently, After washing with PBS, inoculate in a culture bottle and cultivate, wherein the culture medium in the culture bottle is 5% FBS-MSCM medium; after 24 hours, change the medium in full amount, wash twice with PBS to remove miscellaneous cells, and the cell density reaches 80% , the first passage can be carried out; the bone marrow mesenchymal stem cells of the third passage can be collected. Bone marrow mesenchymal stem cells were identified by flow cytometry: the results showed that the third-generation cells all expressed stem cell marker molecules CD44, CD90, and CD105, and the positive rates were 89.4%,...

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Abstract

The invention discloses a preparation method of stem cell preparation for repairing diabetes pancreas islet function, which includes steps of commonly culturing pancreas stem cells and mesenchymal stem cells so as to directionally induce the mesenchymal stem cells to divide towards the pancreatic stem cells; then infusing mesenchymal stem cells after induced directional differentiation into the body intravenously so as to well repair the pancreas islet function of the late diabetes type I and the diabetes type II. The mesenchymal stem cells after induced directional differentiation have the advantages of being high in proliferation potential, easy to acquire, few in ethic limit and low in immunogenicity; after the directional induction, the target property is clear, and the target to repair pancreas islet function is strong. The stem cell applies induced differentiation, and is free from any additive, drugs and factor, thus the safety is high; the cells acquired by induction are seed cells, namely, the stem cells; the preparation method has long-term stability.

Description

technical field [0001] The invention relates to the technical field of stem cell regenerative medicine, in particular to a preparation method of a stem cell preparation for repairing the function of diabetic islets. Background technique [0002] Diabetes mellitus (DM) is a worldwide epidemic, affecting nearly 382 million people worldwide (statistics in 2013), and the number of patients is expected to reach 592 million in 2035. Diabetes can cause a series of serious complications, especially chronic damage and dysfunction of the eyes, heart, blood vessels and nerves, so research on the treatment of diabetes is a global topic. The most important cause of diabetes is the loss of insulin-secreting beta cells in the islets of the pancreas (type I diabetes) or the inability of insulin to function effectively in the body (type II diabetes). Since traditional oral hypoglycemic drugs and exogenous insulin injections cannot fundamentally cure the disease, nor can it completely preven...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775C12N5/074A61K35/39A61P3/10
CPCA61K35/39C12N5/0663C12N5/0678C12N2506/1353C12N2509/00
Inventor 栗彦宁齐锦生张菁
Owner HEBEI MEDICAL UNIVERSITY
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