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A kind of enzyme-catalyzed conversion preparation method of ginsenoside rd

A technology of ginsenoside and catalytic transformation, applied in the field of preparation of ginsenoside Rd, can solve problems such as difficult-to-scale molding, achieve the effect of improving reaction efficiency, overcoming natural sources and difficulty in large-scale preparation

Active Publication Date: 2021-04-09
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the problems of the existing natural sources of ginsenoside Rd and the difficulty in large-scale preparation, so that the enzymatic conversion process of ginsenoside Rb1 occurs on the surface of the macroporous resin, producing very significant beneficial effects

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Take 50ml of ginsenoside Rb1 20 mg solution, add 5ml of pretreated macroporous resin for static adsorption, so that ginsenoside Rb1 is fully adsorbed on the macroporous resin, and then add enzyme-containing aqueous solution to make the enzyme activity greater than 100U / ml, Use hydrochloric acid to adjust the pH to 2, and control the temperature at 40°C for enzymatic hydrolysis. After hydrolysis for 60 minutes, filter, wash with a certain amount of 20% ethanol, elute with 50% ethanol, and wash with detergent and eluent. And elute the macroporous resin, the eluate is concentrated to dryness, dissolved by ultrasonic with 95% ethanol and the volume is adjusted to 10ml. Filter through a 0.45 µm filter head, inject 10 µl of sample, measure by HPLC, and calculate the content, conversion rate and reaction yield of ginsenoside Rb1 and ginsenoside Rd respectively.

Embodiment 2

[0017] The difference from Example 1 is: take 500 mg of American ginseng medicinal material powder and add it to a 25 ml stoppered test tube, adjust the pH to 4 with hydrochloric acid, and carry out enzymatic hydrolysis at 40° C. for 120 minutes.

Embodiment 3

[0019] The difference from Example 2 is that the pH is adjusted to 6 with hydrochloric acid, the temperature is controlled at 40° C. for enzymatic hydrolysis, and the hydrolysis takes 180 minutes.

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PUM

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Abstract

The invention relates to a preparation method of ginsenoside Rd, in particular to a method for preparing ginsenoside Rd by enzymatically transforming ginsenoside Rb1. In view of the fact that the existing biotransformation technology is difficult to achieve large-scale preparation of ginsenoside Rd, ginsenoside Rb1 was immobilized on the macroporous resin for the first time, and then an aqueous solution containing enzyme was added to make the enzyme-catalyzed conversion occur on the surface of the macroporous resin. The conversion process does not contact organic solvents, which not only improves the reaction efficiency, but also the enzyme solution can be used repeatedly, and the conversion rate of ginsenoside Rb1 is 84.04‑97.38%. The preparation method of the invention is simple, low in cost and high in yield, and provides a new method for the large-scale preparation of ginsenoside Rd.

Description

technical field [0001] The invention relates to a preparation method of ginsenoside Rd, in particular to a method for preparing ginsenoside Rd by enzymatically transforming ginsenoside Rb1. Background technique [0002] Ginsenoside Rd is a specific receptor-manipulating calcium ion channel inhibitor, which has therapeutic effects on focal cerebral ischemia, reperfusion injury, prevention and treatment of SHRSP stroke, and anti-neuron cell death and apoptosis. Protective and therapeutic effects, effects on cerebrovascular and cerebral blood flow, effects on energy metabolism of acute cerebral ischemia, effects on hypobaric hypoxia resistance, and reduction of vertebrobasilar artery contraction response caused by 5-HT, clinical Medicines are in high demand. At present, ginsenoside Rd is mainly extracted and separated from medicinal materials such as Chinese medicine Panax notoginseng, and its output is greatly restricted by medicinal material resources. Because ginsenosides ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P33/20
CPCC12P33/005C12P33/20
Inventor 房海燕魏英勤孙新杰
Owner QILU UNIV OF TECH