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DNA/RNA virus PCR real-time enhanced reaction kit, detection reagent and use method of kit

An RNA virus and enhanced reaction technology, applied in the field of detection, can solve the problems of rapid detection of unfavorable on-site samples, time-consuming, cumbersome operation steps, etc., and achieve the effect of saving detection time and labor and high detection efficiency

Inactive Publication Date: 2017-10-20
CHENGDU HAIZHIYUAN BIOTECHNOLOGY CO LTD
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The disadvantage of the existing DNA / RNA virus extraction method is that the operation steps are cumbersome and time-consuming, which is not conducive to the rapid detection of on-site samples; The solution will inhibit the next step of PCR enzyme activity and increase the threshold of the minimum detection sample concentration

Method used

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  • DNA/RNA virus PCR real-time enhanced reaction kit, detection reagent and use method of kit
  • DNA/RNA virus PCR real-time enhanced reaction kit, detection reagent and use method of kit
  • DNA/RNA virus PCR real-time enhanced reaction kit, detection reagent and use method of kit

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Embodiment

[0064] The invention is used for rapid detection of canine parvovirus type 2 gene, and canine parvovirus disease is a highly contagious severe infectious disease of dogs. Clinically, it is characterized by acute hemorrhagic enteritis and myocarditis.

[0065] Canine parvovirus is highly contagious to dogs, and dogs of all ages can be infected. However, dogs with just weaning to 90 days of age are more prone to disease, and the state of an illness is also more serious. According to clinical evidence, some puppies may present symptoms of myocarditis and die suddenly. The disease can occur throughout the year, but occurs frequently in cold winter and spring. The feces of sick dogs contain the highest levels of toxins.

[0066] Canine parvovirus is a single-stranded DNA virus. CPV-2 is the second parvovirus isolated from dogs after Binn et al. in 1967. , MVC) are significantly different in pathogenicity and antigenicity. CPV-2 is highly contagious among domestic dogs and othe...

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Abstract

The invention relates to a DNA / RNA virus PCR real-time enhanced reaction kit, a detection reagent and a use method of the kit, and belongs to the field of detection. The kit aims at DNA / RNA virus samples derived from saliva, feces, blood and nasal secretions without an extraction and purification step, a water bath boiling step or a high temperaturepreheating treatment step. The detection reagent of the PCR real-time enhanced reaction kit comprises ethylphenylpolyethylene glycol, lysozyme, trehalose, sodium acetate trihydrate and water used as a solvent. The detection reagent can promote the release of DNA / RNA from virus particles, the virus detection efficiency of the real-time enhanced PCR reaction kit is high, and the minimum detection concentration of the virus samples is 1 copy.

Description

technical field [0001] The invention relates to a DNA / RNA virus PCR real-time enhanced reaction kit, a detection reagent and a use method thereof, belonging to the detection field. The present invention is aimed at DNA / RNA virus samples derived from saliva, feces, blood, and nasal secretions, without the need for extraction and purification steps, water bath boiling steps, and high-temperature preheating steps. The reagents in this kit promote DNA / RNA from The virus particles are released, and the PCR reaction is enhanced in real time, and the minimum detection concentration of the virus sample is 1 copy. Background technique [0002] Polymerase chain reaction (PCR) is a useful tool for rapid testing of food, environmental, and clinical samples. A typical PCR reaction requires a pretreatment step of DNA extraction and purification of the sample to remove many factors that inhibit the PCR reaction. This pretreatment step usually takes 1 to 2 hours to complete. [0003] Comm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68
CPCC12Q1/686C12Q1/70
Inventor 莫邦辉唐闻静
Owner CHENGDU HAIZHIYUAN BIOTECHNOLOGY CO LTD
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