Method for inhibiting CAS genetic expressions of Siraitia grosvenorii

A technology of gene expression and Luo Han Guo, applied in the field of plant biology, can solve the problem of low yield of mogroside V, achieve the effect of increasing accumulation, increasing content, and promoting synthesis

Inactive Publication Date: 2017-10-24
韦荣昌
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] An object of the present invention is to solve the problem of low yield of mogroside V in traditional cultivation and to provide at least the advantages which will be explained later

Method used

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  • Method for inhibiting CAS genetic expressions of Siraitia grosvenorii

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Embodiment 1

[0022] A method for inhibiting the expression of the CAS gene of Luo Han Guo, inoculating the tissue cultured seedlings of Luo Han Guo in the induction medium with a methyl jasmonate concentration of 325 μmol / L, placing the humidity at 60%, the light intensity at 1200 lux, the light time at 8 h / d, and the temperature at 23 Cultured at ±2°C for 30 days. The solid medium components are: MS+6-BA 1.5mg / L+indole butyric acid 0.3mg / L+agar 3.5g / L+sucrose 30g / l+activated carbon 1.0g / L; the method of adding methyl jasmonate is: Dissolve methyl jasmonate in aqueous ethanol solution with a volume fraction of 2% to prepare methyl jasmonate mother liquor, and sterilize with a 0.22 μm microporous membrane, cool to 24-26 °C after sterilization, and then cool the methyl jasmonate The ester mother solution was added to the above solid medium until the final concentration of methyl jasmonate was 325 μmol / L to obtain an induction medium.

Embodiment 2

[0024] A method for inhibiting the expression of the CAS gene of Luo Han Guo. The tissue cultured seedlings of Luo Han Guo are inoculated in the induction medium with a methyl jasmonate concentration of 375 μmol / L, placed in a humidity of 65%, light intensity of 1400 lux, light time of 8 h / d, and a temperature of 23 Cultured at ±2°C for 30 days. The solid medium components are: MS+6-BA 1.5mg / L+indole butyric acid 0.3mg / L+agar 3.5g / L+sucrose 30g / l+activated carbon 1.0g / L; the method of adding methyl jasmonate is: Dissolve methyl jasmonate in aqueous ethanol solution with a volume fraction of 2% to prepare methyl jasmonate mother liquor, and sterilize with a 0.22 μm microporous membrane, cool to 24-26 °C after sterilization, and then cool the methyl jasmonate The ester mother solution was added to the above solid medium until the final concentration of methyl jasmonate was 375 μmol / L to obtain an induction medium.

Embodiment 3

[0026] A method for inhibiting the expression of the CAS gene of Luo Han Guo, inoculating the tissue cultured seedlings of Luo Han Guo in a solid medium containing methyl jasmonate at a concentration of 400 μmol / L, placing the humidity at 66%, the light intensity at 1400 lux, the light time at 8 h / d, and the temperature Cultivate for 30 days at 23±2°C. The solid medium components are: MS+6-BA 1.5mg / L+indole butyric acid 0.3mg / L+agar 3.5g / L+sucrose 30g / l+activated carbon 1.0g / L; the method of adding methyl jasmonate is: Dissolve methyl jasmonate in aqueous ethanol solution with a volume fraction of 2% to prepare methyl jasmonate mother liquor, and sterilize with a 0.22 μm microporous membrane, cool to 24-26 °C after sterilization, and then cool the methyl jasmonate The ester mother solution was added to the above solid medium until the final concentration of methyl jasmonate was 400 μmol / L to obtain an induction medium.

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Abstract

The invention discloses a method for inhibiting the expression of the CAS gene of Luo Han Guo. The tissue cultured seedlings of Luo Han Guo are inoculated in an induction medium with a methyl jasmonate concentration of 325-400 μmol/L and cultivated for 28-30 days. The invention has the functions of inhibiting the expression of the mogroside CAS gene and increasing the content of mogroside V.

Description

technical field [0001] The present invention relates to the field of plant biology. More specifically, the present invention relates to a method for inhibiting the expression of the CAS gene of Luo Han Guo. Background technique [0002] Mogroside V is extracted from the specialty economic plant in Guangxi—Mogros grosvenori. Its sweetness is 300 times that of sucrose, and its calories are zero. Prevention and control effect, the current market demand for mogroside V is large, so it is necessary to improve the planting efficiency to increase the output of mogroside V to meet the needs of the market. Mogroside V belongs to the cucurbitane-type tetracyclic triterpenoids. Our research group has deduced the possible biosynthetic pathway of Mogroside V based on the transcriptome data of Luo Han Guo. The precursors for the biosynthesis of mogroside V are isopentenyl diphosphate (IPP) and 3,3-dimethylenylpyrophosphate (DMAPP), both of which are produced by mevalonate (MVA) and meth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/06A01H4/00
CPCA01H4/008A01G7/06A01H4/001
Inventor 韦荣昌黄小华
Owner 韦荣昌
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