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Detection method for preparing spiny platinum-coated gold nanorod chiral dimer for DNA damage

A DNA damage, gold nanorod technology, used in food safety, analytical chemistry

Active Publication Date: 2017-10-24
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of nucleic acid to assemble to form thorny platinum-coated gold nanorod dimers produces circular dichroism spectral signals in the visible / near-infrared region, which is still blank for the detection of DNA damage by biotoxins and their metabolites

Method used

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  • Detection method for preparing spiny platinum-coated gold nanorod chiral dimer for DNA damage
  • Detection method for preparing spiny platinum-coated gold nanorod chiral dimer for DNA damage
  • Detection method for preparing spiny platinum-coated gold nanorod chiral dimer for DNA damage

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Embodiment 1

[0033] Example 1 Preparation of spiny platinum-coated gold nanorod chiral dimers for DNA damage detection

[0034] (1) Synthesis of gold nanorods

[0035] Gold nanorods with an aspect ratio of about 3.0 were synthesized according to the method of the patent "A Preparation Method of Self-Assembled Materials with Surface-Enhanced Raman Activity" (Patent No.: ZL201010605799.9). The simple steps are as follows:

[0036] ① Synthesis of seed crystals: Add 0.1 mL of pre-configured 2 g / L tetrachloroauric acid trihydrate to 1 mL of 0.2 M cetyltrimethylammonium bromide solution at 28.0 °C. At this time, The color of the solution changed from colorless to yellowish brown. Then add 0.12 mL of newly prepared 0.01 M sodium borohydride solution to the above mixed system and stir rapidly for 2 min. The color of the solution changed from yellowish brown to light brown. Put the above synthesized seed crystal into a water bath at 28.0°C and let it stand for 30 minutes to react, so that the s...

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Abstract

The invention discloses a detection method for preparing a spiny platinum-coated gold nanorod chiral dimer for DNA damage and belongs to the technical field of analytical chemistry and the technical field of food security. The method comprises the steps of (1) synthesis of a gold nanorod; (2) synthesis of a spiny platinum-coated gold nanorod; (3) surface-oriented nucleic acid functionalization of the spiny platinum-coated gold nanorod; (4) establishment of a spiny platinum-coated gold nanorod chiral dimer; (5) pre-damage of biotoxin to a DNA; and (6) detection of the DNA damage by using the spiny platinum-coated gold nanorod chiral dimer. A plasma chiral spectroscopy signal can be successfully applied to the field of biosensing detection. The spiny platinum-coated gold nanorod chiral dimer assembled by using a nucleic acid generates a circular dichromatic spectrum signal of a visible light / near-infrared region, thereby being applied to detection of the DNA damage through biotoxin and metabolites thereof.

Description

technical field [0001] The invention discloses a method for detecting DNA damage by preparing thorny platinum-wrapped gold nanorod chiral dimers, which belongs to the technical field of analytical chemistry and the technical field of food safety. Background technique [0002] DNA is an important genetic material of a living body, but DNA damage often occurs in a living body. Exogenous chemical or biological substances and their metabolites, such as biotoxins and their metabolites, often cause oxidation (base oxidation, base loss or single or double strand breaks) and / or base Structural damage including modifications (base adducts, base methylation, or formation of pyrimidine dimers). Such damaged DNA can easily cause gene mutation and even cause cancer of the body. Therefore, the development of DNA damage detection is closely related to environmental monitoring and food safety (especially cross-border food safety). At present, it is urgent to develop a new rapid detection ...

Claims

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Application Information

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IPC IPC(8): G01N21/21G01N33/53B22F1/02B82Y5/00B82Y30/00B82Y40/00
CPCG01N21/21G01N33/5308B82Y5/00B82Y30/00B82Y40/00G01N2021/216B22F1/17
Inventor 徐丽广胥传来匡华刘丽强马伟宋珊珊吴晓玲胡拥明
Owner JIANGNAN UNIV
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