A kind of inducing differentiation medium and method for inducing fat stem cells to differentiate into chondrocytes

A technique for inducing differentiation of adipose stem cells, which is applied in the field of inducing differentiation medium for inducing the differentiation of adipose stem cells into chondrocytes, can solve the problems of low expression efficiency of type II collagen, increase chondrocyte anabolism, and low differentiation rate, and achieve the promotion of cartilage Cell anabolism and chondrogenesis, low cost, improved expression effect
CN107338218BActive Publication Date: 2020-11-24FIRST HOSPITAL AFFILIATED TO GENERAL HOSPITAL OF PLA
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
FIRST HOSPITAL AFFILIATED TO GENERAL HOSPITAL OF PLA
Publication Date
2020-11-24

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Abstract

The invention discloses an inducer and a method for inducing differentiation of adipose-derived stem cells to cartilage cells, belonging to the technical field of cell biology. According to the technical scheme, the inducer comprises TGF-Beta 3, FGF-18, dexamethasone, insulin, selenium, transferrin, linoleic acid, BSA, sodium pyruvate solution, L-ascorbic acid-2-phosphate and proline. The inducer and the method have the advantages that the synthesis and metabolism of the cartilage cells are rapidly promoted, the generation of cartilage is promoted, the expression of cartilage extracellular matrix II type collagen and glycosaminoglycan is remarkably improved, and the time required for in-vitro induction culture is reduced; and the inducer and the method are widely suitable for various culture systems, especially a pellet system and a three-dimensional cell scaffold culture system.
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Description

technical field

[0001] The invention relates to the technical field of cell biology, in particular to a differentiation medium and a method for inducing the differentiation of fat stem cells into chondrocytes. Background technique

[0002] Articular cartilage is mainly composed of chondrocytes and extracellular matrix (ECM). It is a special form of connective tissue. Its function is to reduce the pressure on the subchondral bone during joint movement and reduce the friction of the articular surface. Since cartilage tissue has no blood supply, it can only rely on joint fluid to provide nutrition. Therefore, once cartilage tissue is damaged, its ability to regenerate and repair is extremely poor, and it is difficult to heal itself. There are many reasons for cartilage damage, such as external impact, autoimmune diseases such as rheumatoid arthritis, body degeneration caused by aging, etc., resulting in a high incidence of cartilage damage and high treatment costs, which is a m...

Claims

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