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Method for preparing fatty glyceride

A technology of fatty acid glycerides and free fatty acids, which is applied in the field of synthetic fatty acid glycerides, can solve the problems of long time required for equilibrium and low conversion rate of fatty acids, and achieve the effects of reducing separation and purification steps, increasing conversion rate, and high efficiency

Inactive Publication Date: 2017-11-10
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims at the disadvantages of low fatty acid conversion rate and long time required for the esterification reaction to reach equilibrium in the existing enzymatic esterification reaction method, and provides a method for catalytically preparing fatty acid glycerides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 (two-step enzymatic method)

[0026] The fatty acid by-products in deep-sea fish oil processing are taken as raw materials, and the fatty acid content is 99.5%, and the DHA content is 20% and the EPA content is 22%. Take 100g of this fatty acid and 100g of glycerin and mix them in a reaction vessel, add 4g of Lipozyme TL 100L (a lipase derived from Thermomyces lanuginosus) and 5g of water, put them in a glycerin bath at 50°C and react for 2h under stirring , The reaction esterification rate detected by liquid chromatography is 62%. Centrifuge the oil phase and glycerol phase in the above reaction, keep the glycerol phase for recycling, and dehydrate the oil phase; take 100g of the above oil phase and place it in a reaction vessel, add 1.6g Novozym435 lipase (a lipase derived from Candida antarctica) ), placed in a constant temperature magnetic stirrer at 55°C, and stirred and reacted for 5h under a vacuum of 100pa to obtain a reaction product, which was de...

Embodiment 2

[0027] Embodiment 2 (two-step enzymatic method)

[0028] Get the deodorized effluent in the deodorization and refining process of soybean oil as the reaction raw material, wherein the fatty acid content is 91.3%, get the compound 500g, glycerol 600g, water 11g, 5 g of L (lipase derived from Candida antarctica) was mixed and then placed in a reactor at 50° C. for a stirring reaction for 4 hours. The esterification rate of this reaction was detected by liquid chromatography to be 59%. The oil phase and glycerin phase in the above reaction were separated by centrifugation, the glycerin phase was retained for recycling, and the oil phase was dehydrated; 300 g of the above oil sample was added to 10 g of Novozym 435 lipase, stirred and reacted under 100 Pa vacuum for 4 hours to obtain the reaction product, the liquid phase Chromatographic detection shows that in the product at this time, the TAG content is 41.5%, the DAG content is 52.27%, the MAG is 3.51%, and the FFA is 2.74%. T...

Embodiment 3

[0029] Embodiment 3 (two-step enzymatic method)

[0030] Take acidified oil, a by-product of soybean oil refining, in which the fatty acid content is 51.5%, and the triglyceride content is 48.3%. Take 500g of the compound, 250g of glycerin, 11g of water, 5g of Lipozyme TL 100L lipase, mix and place in the reactor The reaction was stirred at 45°C for 4h. Centrifuge the oil phase and glycerin phase in the above reaction, keep the glycerin phase for recycling, and dehydrate the oil phase; take 400g of the above oil sample, add 8g of Novozym435 lipase, stir and react under 100Pa vacuum for 4h to obtain the reaction product, liquid chromatography It was detected that in the product at this time, the TAG content was 66.5%, the DAG content was 30.27%, the MAG content was 2.09%, and the FFA content was 1.14%; the esterification rate of fatty acid was 97.78%.

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PUM

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Abstract

The invention discloses a method for preparing fatty glyceride. The method comprises steps as follows: (1) glycerinum and free fatty acid in the mass ratio being (1-5):1 are mixed, free lipase accounting for 0.1%-2% of the total mass of a reactant and water accounting for 1%-6% of the total mass of the reactant are added, and the mixture is stirred to react at the temperature of 30-60 DEG C for 2-4 h; (2) an oil phase and a glycerinum phase in the reaction are subjected to centrifugal separation, the glycerinum phase is retained to be recycled, and the oil phase is dehydrated; (3) immobilized lipase accounting for 1%-5% of the total mass of the oil phase is added to the dehydrated oil phase, the mixture is subjected to a high-vacuum reaction at the temperature of 45-65 DEG C for 4-8 h, and fatty glyceride is obtained. Fatty glyceride is esterified and synthesized with a two-step method, the conversion rate of fatty acid in the final product is 98% or higher, the conversion rate of the fatty acid is increased, the separation and purification steps are omitted, and the method has good economic value and environmental protection value.

Description

technical field [0001] The invention relates to a method for synthesizing fatty acid glycerides. Background technique [0002] Glycerides are the most important lipid components in human and animal diets, especially in vegetable oils and animal oils, where the content of glycerides is usually greater than 90%, or even higher than 99%. Free fatty acids are widely found in various oils. In common animal and vegetable oils, the content of free fatty acids can reach 1% to 5%, and in some high acid value oils, it can reach more than 25%. During oil processing, free fatty acids are separated as by-products, which produces a large amount of free fatty acid products of natural animal and vegetable oils. Free fatty acids are unstable in nature and generally have a bad smell during processing, so they cannot be used as animal feed or human food raw materials. Therefore, the development of a suitable process to convert fatty acids into glycerides is an important subject of long-term ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
CPCC12P7/6454
Inventor 王永华连伟帅朱东奇李道明严慧玲杨博王卫飞
Owner SOUTH CHINA UNIV OF TECH
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