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A kind of detoxification and micropropagation method of Jiabao fruit

A micropropagation and Gerber technology, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of long reproduction cycle, low multiplication efficiency, low seedling breeding efficiency, etc., and achieve low cost and high multiplication efficiency Effects of high and significant market potential and economic benefit prospects

Inactive Publication Date: 2019-06-11
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved in the present invention is to overcome the problems and technical deficiencies in the above-mentioned existing jabocarpa seedling breeding, utilize tissue culture culture technology, through the establishment of detoxification method, micro branch culture, proliferation culture and rooting culture, Established a virus-free micropropagation method for Jiabao fruit, which can effectively solve the problems of low seedling breeding efficiency, virus, seasonal restrictions, long reproductive cycle, low multiplication efficiency, high cost and poor genetic stability. The industrial production of seedlings and the introduction of virus-free germplasm resources of Jiabao fruit laid the foundation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A kind of detoxification and micropropagation method of Jiabao fruit, the steps are as follows:

[0039] ① Disinfection of explants. First, clean the twigs of Gerberia japonica collected from domestic planting bases with detergent, then rinse with running water until clean, then soak in 75% ethanol solution for 30s, and then rinse with sterile water for 3 times , and then sterilized with a compound disinfectant containing 1% Tween and 0.1% mercuric chloride for 7 minutes, and then rinsed with sterile water for 3 times to form sterile explants.

[0040]②Proliferation culture, which inoculates the sterilized Jabocaria explants on the MS solid medium as the basic medium, and also contains 0.5mg / L of 6-BA, 0.05mg / L of TDZ, 0.05mg / L NAA and sucrose with a mass concentration of 3.0% and a proliferation medium with a pH value of 5.6 were used for proliferation culture.

[0041] ③ For rooting culture, microbranches with a growth length of 2.0-3.0 cm were inoculated on MS solid...

Embodiment 2

[0046] A kind of detoxification and micropropagation method of Jiabao fruit, the steps are as follows:

[0047] ① Disinfection of explants. Clean the twigs of Jabocaria imported from abroad first with detergent, then rinse with running water until clean, then soak in 75% ethanol solution for 60s, then rinse with sterile water for 5 times, and then Disinfect with a compound disinfectant containing 1% Tween and 5% sodium hypochlorite for 7 minutes, and then rinse with sterile water 5 times to obtain sterile explants.

[0048] ② Proliferation culture, which inoculates the sterilized Jabocaria explants on the HS solid medium as the basic medium, and also contains 3.0 mg / L of 6-BA, 0.5 mg / L of TDZ, 0.1 mg / L NAA and sucrose with a mass concentration of 3.0% and a proliferation medium with a pH value of 6.0 were used for proliferation culture.

[0049] ③ For rooting culture, microbranches with a growth length of 2.0-3.0 cm were inoculated on HS solid medium as the basic medium, whic...

Embodiment 3

[0054] A kind of detoxification and micropropagation method of Jiabao fruit, the steps are as follows:

[0055] ① Disinfection of explants. The seeds of Jabocaria imported from abroad are first cleaned with detergent, rinsed with running water until clean, then soaked in 75% ethanol solution for 60s, then rinsed with sterile water for 5 times, and then rinsed with The compound disinfectant with a mass concentration of 1% Tween and 0.1% mercuric chloride was sterilized for 15 minutes, and then washed 5 times with sterile water to obtain sterile explants.

[0056] ② Proliferation culture, which inoculates the sterilized Jabocaria explants on the HS solid medium as the basic medium, and also contains 0.5 mg / L 6-BA, 0.1 mg / L TDZ, 0.1 mg / L NAA and sucrose with a mass concentration of 3.0% and a proliferation medium with a pH value of 6.0 were used for proliferation culture.

[0057] ③ For rooting culture, inoculate microbranches with a growth length of 2.0 to 3.0 cm on HS solid me...

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Abstract

The invention belongs to the field of seed seedling, agricultural biological technology, and tropic valuable and rare fruit tree seedling breeding, and discloses a virus-free micro-breeding method of jaboticaba. According to the virus-free micro-breeding method, plant virus-free breeding and tissue culture are adopted. The virus-free micro-breeding method comprises steps of explant disinfection, propagation and culturing, rooting, hardening seedling, and transplanting and seedling growing. The virus-free micro-breeding method is short in breeding period, high in proliferation rate; obtained seedlings contain no virus; seedling characters are uniform; cost is low; rooting rate and transplanting survival rate are high; growth period is not limited by seasons; problems in jaboticaba germplasm resource introduction and seedling breeding such as long breeding period, low propagation efficiency, virus carrying, and high cost are solved; the virus-free micro-breeding method possesses significant meaning on jaboticaba seedling breeding and popularization, and possesses excellent market potential and promosing economic benefit prospect.

Description

technical field [0001] The invention belongs to the technical field of plant seedling breeding. More specifically, it relates to a virus-free micropropagation method for jabo fruit. Background technique [0002] Gerbera ( Myrciaria cauliflora Berg), aliased as treasure fruit, tree grapes, and Myrtle, etc., the English name is "Jaboticaba", which is a tropical and subtropical fruit of the genus Myrtaceae, which is native to Brazil and other countries in South America. Known as the "King of Fruits", it is rich in anthocyanins, flavonoids, vitamin C, vitamin B, thiamine, niacin, calcium, phosphorus, iron and other nutrients. In addition to eating fruit, it can also be processed into jelly, candied fruit, jam, fruit wine, flavored food, food additives, etc. In recent years, there have been reports that it has been developed into health food and cosmetics. In developed countries such as Europe, America, Japan, Taiwan, Hong Kong, etc. And regions have important market potential...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 胡伟丁泽红铁韦韦颜彦
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI