A kind of method for extracting active protein and glutathione from Tongkat Ali

Abstract

A method for extracting active proteins and eurycomanone from Eurycoma longifolia comprises the following steps: (1) crushing a raw material, carrying out continuous countercurrent extraction, and cooling and filtering the extracted raw material to obtain an extract; (2) carrying out centrifuging and ultrafiltration on the extract, adding water, and rinsing the treated extract to obtain a treated liquid; (3) carrying out nanofiltration and concentration to obtain a concentrate a; (4) adding the concentrate a into an alcohol solution, carrying out stationary alcohol precipitation, centrifuging the obtained solution, filtering the centrifuged solution, drying obtained filter residues to obtain the Eurycoma longifolia active proteins, decolorizing the obtained filtrate with active carbon, centrifuging the decolorized filtrate, and concentrating the centrifuged filtrate to obtain a concentrate b; (5) adding the concentrate b to an alumina column, carrying out chromatography, washing the column with water, carrying out gradient elution by using an organic solvent, and concentrating the obtained eluate to obtain extraction extract; and (6) dissolving the extraction extract, introducing the obtained solution into a liquid chromatograph, carrying out elution, collecting the obtained target eluate, concentrating the target eluate, and drying the concentrated target eluate to obtain the highly pure eurycomanone product. The purity and yield of the water-soluble proteins and the eurycomanone product obtained through the method are high; and the method has the advantages of simple operating process, low cost, and realization of continuous large-scale production.

Description

technical field [0001] The invention relates to a method for extracting active protein and ketone, in particular to a method for extracting active protein and ketone from Tongkat Ali. Background technique [0002] Tongkat Ali is a plant of the genus Tongkat Ali in the bitter wood family, also known as Malaysian ginseng, native ginseng and natural Viagra. Tongkat Ali is a shrub that grows in moist sandy soil in the primitive tropical rainforest near the equator. It is mainly distributed in Southeast Asia such as Malaysia and Indonesia. The maturity period is generally more than 5 years. The root is often used as medicine, and together with bird's nest and tinware, it is called the three national treasures of Malaysia. Traditionally, in Malaysia, Tongkat Ali is often used as an aphrodisiac, tonic, antipyretic and antidote to poisoning or snake venom, and has significant effects. Modern medical research has proved that the active ingredients of Tongkat Ali extract have signif...

AI Technical Summary

Problems solved by technology

Although the cost of water extraction of Tongkat Ali is low, the operation is simple, and the separation and purification of macroporous adsorption resin can also purify ketanthanone to a certain extent, but there are problems such as low extraction rate and poor adsorption selectivity, and the degree of purity improvement is limited.