Soybean protein hydrolyzate, preparation method and applications thereof

A technology of soybean protein and hydrolyzate, which is applied in the field of soybean protein hydrolyzate and its preparation, can solve the problems of uneven product quality and performance, affect the application of soybean protein, and difficult to control the fermentation process, etc., to overcome the uneven product performance , Promote self-synthesis ability, enhance the effect of skin protection

Inactive Publication Date: 2017-11-21
广州德臻生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Soy protein is a kind of protein (soyprotein) derived from soybean. It is a vegetable protein and has the characteristics of less methionine. The amino acid composition of soybean protein is similar to that of milk, and its amino acid gene structure is very close to that of human amino acids. Protein; at the same time, soybean protein dry matter is a mixture of soybean protein and other soybean ingredients. Its ingredients are mainly protein and contain other soybean ingredients. It has the effect of preventing hyperplasia, and at the same time, the estrogen-like effect has the effect of making the skin smooth, delicate, soft and elastic. Therefore, there are some cosmetics added

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  • Soybean protein hydrolyzate, preparation method and applications thereof
  • Soybean protein hydrolyzate, preparation method and applications thereof
  • Soybean protein hydrolyzate, preparation method and applications thereof

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Effect test

Embodiment 1

[0022] Embodiment 1 soybean protein hydrolyzate and hydrolysis method thereof

[0023] The preparation method of the soybean protein hydrolyzate of the present embodiment may further comprise the steps:

[0024] 1. Take soybean protein (protein content ≥ 55%, purchased from Shijiazhuang Chunxin Biotechnology Co., Ltd.) 100g and recombinant cathepsin L (manufacturer BioVision) 100mg, add water to 1000ml;

[0025] 2. Adjust the pH value to 6.8, stir at room temperature for 30 minutes, then place it in a warm water bath at 37°C, and carry out hydrolysis reaction for 24 hours while stirring to obtain 1000ml of soybean protein cathepsin hydrolyzate;

[0026] 3. Routine vacuum drying and concentration to obtain 100ml liquid protein hydrolyzate;

[0027] 4. Take 1ml of the liquid protein hydrolyzate and use GPC method (instrument: Agilent, chromatographic column: PLgel 5um MIXED-C) to measure the molecular weight. It is confirmed that the molecular weight distribution is 400-3000, a...

Embodiment 2

[0028] Embodiment 2 soybean protein hydrolyzate and hydrolysis method thereof

[0029] The preparation method of the soybean protein hydrolyzate of the present embodiment may further comprise the steps:

[0030] 1. Take soybean protein (protein content ≥ 55%, purchased from Shijiazhuang Chunxin Biotechnology Co., Ltd.) 50g and recombinant cathepsin L (manufacturer BioVision) 100mg, add water to 1000ml;

[0031] 2. Adjust the pH value to 7.2, stir at room temperature for 20 minutes, then place it in a warm water bath at 38°C, and carry out hydrolysis reaction for 20 hours while stirring to obtain 1000ml of soybean protein cathepsin hydrolyzate;

[0032] 3. Routine vacuum drying and concentration to obtain 100ml liquid protein hydrolyzate;

[0033] 4. Take 1ml of the liquid protein hydrolyzate and use GPC method (instrument: Agilent, chromatographic column: PLgel 5um MIXED-C) to measure the molecular weight. It is confirmed that the molecular weight distribution is 400-3000, an...

Embodiment 3

[0034] Embodiment 3 soybean protein hydrolyzate and hydrolysis method thereof

[0035] The preparation method of the soybean protein hydrolyzate of the present embodiment may further comprise the steps:

[0036] 1. Take soybean protein (protein content ≥ 55%, purchased from Shijiazhuang Chunxin Biotechnology Co., Ltd.) 10g and recombinant cathepsin L (manufacturer BioVision) 100mg, add water to 1000ml;

[0037] 2. Adjust the pH value to 7.8, stir at room temperature for 50 minutes, then place in a warm water bath at 42°C, carry out hydrolysis reaction for 18 hours while stirring, and obtain 1000ml of soybean protein cathepsin hydrolyzate;

[0038] 3. Routine vacuum drying and concentration to obtain 100ml liquid protein hydrolyzate;

[0039] 4. Take 1ml of the liquid protein hydrolyzate and use GPC method (instrument: Agilent, chromatographic column: PLgel 5um MIXED-C) to measure the molecular weight. It is confirmed that the molecular weight distribution is 400-3000, and the...

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PUM

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Abstract

The invention discloses a soybean protein hydrolyzate, a preparation method and applications thereof. The preparation method comprises: adding cathepsin L to soybean protein, adjusting the pH value to 5.5-8.5, stirring for 20-60 min at a room temperature, and carrying out a reaction for 2-24 h under a water bath condition of 35-42 DEG C so as to obtain the soybean protein hydrolyzate. According to the present invention, the soybean protein cathepsin L hydrolyzate can effectively improve the expression amounts of COL1A1 gene, COL1A2 gene and KPRP gene, can effectively increase the collagen I content in skin while enhance the protective function of skin, and can finally achieve effects of skin aging alleviating, skin protection improving and correspondingly improved skin moisturizing so as to achieve the purpose of skin care; with the estrogen-like effect of the soybeans, a lot of effects can be provided for the maintaining of the health of the skin; through the synergetic effect of a variety of factors and other unknown mechanisms, the soybean protein hydrolyzate of the present invention can effectively maintain the skin, and can alleviate the symptoms of various skin diseases.

Description

technical field [0001] The invention belongs to the technical field of skin care products and relates to a soybean protein hydrolyzate and its preparation method and application. Background technique [0002] Soy protein is a kind of protein (soyprotein) derived from soybean. It is a vegetable protein and has the characteristics of less methionine. The amino acid composition of soybean protein is similar to that of milk, and its amino acid gene structure is very close to that of human amino acids. Protein; at the same time, soybean protein dry matter is a mixture of soybean protein and other soybean ingredients. Its ingredients are mainly protein and contain other soybean ingredients. It has the effect of preventing hyperplasia, and at the same time, the estrogen-like effect has the effect of making the skin smooth, delicate, soft and elastic. Therefore, there are some cosmetics added with fermented soybean protein on the market, but soybean fermentation needs to be fermente...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K2/00A61K8/64A61Q19/00A61Q19/10
CPCA61K8/645A61Q19/00A61Q19/10C07K2/00C12P21/06
Inventor 金京勋崔红易梅仙易其
Owner 广州德臻生物技术有限公司
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