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Novel water soluble natural polysaccharide antibacterial material and preparation method thereof

A technology of natural polysaccharides and antibacterial materials, applied in botany equipment and methods, chemicals for biological control, biocides, etc., can solve the problem that the antibacterial performance has not been significantly improved, so as to avoid the decline of antibacterial effect and operate Simple, less cytotoxic effects

Active Publication Date: 2017-11-24
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods have solved the water-solubility problem of chitosan well, antibacterial performance has not improved obviously.

Method used

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  • Novel water soluble natural polysaccharide antibacterial material and preparation method thereof
  • Novel water soluble natural polysaccharide antibacterial material and preparation method thereof
  • Novel water soluble natural polysaccharide antibacterial material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Add 0.5 gram of chitosan to 100 milliliters of dilute hydrochloric acid with a concentration of 0.1 mol / L, and mechanically stir for half an hour under the condition of an oil bath at 60° C., so that the chitosan is completely dissolved, thereby obtaining a chitosan concentration of 0.005 g / L. mL of a homogeneous solution; the oil bath was heated to 110°C, and 1.3 grams of dicyandiamide was added to the chitosan solution at one time, and the molar ratio of dicyandiamide to chitosan was 5:1, and kept stirring at a constant temperature for 6 hours; The reaction solution was cooled to room temperature, and then lysine, N-hydroxysuccinimide (NHS) and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide activated at room temperature for 2 hours were Mixed solution of hydrochloride (EDC·HCl) (the solvent is a buffer solution of 2-(N-morpholino)ethanesulfonic acid (MES) with a concentration of 30mmol / L, the pH value is about 5.0) 20mL is added to the above reaction solution In the pr...

Embodiment 2

[0056] Add 1.0 gram of chitosan into 100 milliliters of dilute hydrochloric acid with a concentration of 0.1 mol / L, and mechanically stir for one hour under an oil bath condition of 60° C., so that the chitosan dissolves completely, thereby obtaining a chitosan concentration of 0.01 g / L. mL of a homogeneous solution; the oil bath was heated to 105°C, and 1.05 grams of cyanamide was added once to the chitosan solution system, the molar ratio of cyanamide to chitosan was 4:1, and kept stirring at a constant temperature for 6 hours; then oil The reaction solution in the bath was cooled to room temperature, and the leucine, N-hydroxysuccinimide (NHS) and 1-(3-dimethylaminopropyl)-3-ethane activated for 3 hours were Add 20ml of a mixed solution of carbodiimide hydrochloride (EDC·HCl) (the buffer solution of 2-(N-morpholino)ethanesulfonic acid (MES) with a solvent of 30mmol / L) into the above reaction solution, Continue to stir and react at room temperature for 10 hours, wherein the ...

Embodiment 3

[0058] Add 2.0 grams of chitosan to 100 milliliters of dilute hydrochloric acid with a concentration of 0.15 mol / L, and mechanically stir for one hour under an oil bath condition of 60° C., so that the chitosan dissolves completely, thereby obtaining a chitosan concentration of 0.02 g / L. mL of uniform solution; heat the oil bath to 100°C, add 2.08 grams of dicyandiamide to the chitosan oil bath system at one time, the molar ratio of dicyandiamide to chitosan is 2:1, under the condition of 100°C oil bath Stir at constant temperature for 12 hours; then the reaction solution in the oil bath was cooled to room temperature, and the isoleucine, N-hydroxysuccinimide (NHS) and 1-(3-dimethyl Aminopropyl)-3-ethylcarbodiimide hydrochloride (EDC·HCl) mixed solution (the solvent is the buffer of 2-(N-morpholino)ethanesulfonic acid (MES) with a concentration of 30mmol / L Solution, pH value is about 5.0) 20ml is added to the above-mentioned reaction solution, and the stirring reaction is cont...

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Abstract

The invention discloses a novel water soluble natural polysaccharide antibacterial material. The molecules of the novel water soluble natural polysaccharide antibacterial material not only have guanidyl but also have amino acid groups, so that the biosafety of the novel water soluble natural polysaccharide antibacterial material is considered while the antibacterial property of chitosan is improved, and the novel water soluble natural polysaccharide antibacterial material is small in cytotoxicity and is a green antibacterial product. The invention also discloses a method of preparing the material. The method comprises the following steps: 1) dissolving chitosan in a diluted acid solution to obtain a diluted acid aqueous solution of chitosan; 2) adding cyanamide or dicyanamide into the diluted acid solution of chitosan for reaction; 3) adding an amino acid activated solution into a reaction system in the step 2) for amidation; 4) adding hydroxylamine hydrochloride for terminating reaction; and 5) dialyzing a reaction liquid which is filtered with deionized water, and performing microwave vacuum drying treatment to obtain the novel water soluble natural polysaccharide antibacterial material. The novel water soluble natural polysaccharide antibacterial material can be prepared in a reaction kettle by a primary reaction, and the used primary raw materials are rich in source and low in price and are suitable for industrial production.

Description

technical field [0001] The invention relates to the field of chitosan preparation, in particular to a novel water-soluble natural polysaccharide antibacterial material and a preparation method thereof. Background technique [0002] Chitosan, whose chemical name is polyglucosamine (1-4)-2-amino-B-D glucose, is a product obtained after deacetylation of chitin contained in the shells of crustaceans such as shrimp and crabs and fungal cell walls. A natural alkaline polysaccharide. Chitosan has excellent biocompatibility and biodegradability, and can be easily made into various derivatives. Because of its extremely rich sources, it can be dissolved in hydrochloric acid, acetic acid and other organic acids, and has been widely used in industrial and medical fields. Due to its biodegradability, biocompatibility, biological non-toxicity and antibacterial activity, chitosan has become one of the research hotspots in the development of natural antibacterial agents in recent years. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08A01N43/16A01P1/00
CPCA01N43/16C08B37/003
Inventor 牛忠伟蒋士冬
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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