Application of glycosyl transferase UGT84A2 of Arabidopsis in regulation of flowering time of plants

A technology of Arabidopsis sugar and base transfer, applied in angiosperm/flowering plants, transferase, application, etc., can solve problems such as undiscovered

Inactive Publication Date: 2017-11-24
LINYI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the role of other Arabidopsis glycosyltransferases in regulating flowering time has not been identified so far

Method used

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  • Application of glycosyl transferase UGT84A2 of Arabidopsis in regulation of flowering time of plants
  • Application of glycosyl transferase UGT84A2 of Arabidopsis in regulation of flowering time of plants
  • Application of glycosyl transferase UGT84A2 of Arabidopsis in regulation of flowering time of plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Tissue specific expression analysis of Arabidopsis UGT84A2

[0045] 1. UGT84A2P::GUS recombinant plasmid construction and its transgenic homozygous strain obtained

[0046] The promoter sequence of the UGT84A2 gene was amplified from the wild-type Arabidopsis Col-0 genome (the promoter sequence is shown in SEQ ID NO: 5, and the amplification primers are shown in SEQ ID NO: 6 and SEQ ID NO: 7, respectively. Show), named UGT84A2P. The UGT84A2P promoter sequence was cloned and connected to the intermediate vector PBSK. After PCR verification and restriction enzyme digestion verification, the sequence was sent. The UGT84A2 promoter sequencing result was compared with the published sequence in the TAIR database, and the result was 100% identical, which proved that the cloned promoter sequence was completely correct and could be used to construct a plant expression vector for the GUS reporter gene. The cloning vector was digested with enzymes and then subjected to agar...

Embodiment 2

[0050] Example 2 Cloning of Arabidopsis glycosyltransferase gene UGT84A2, overexpression body and transgenic strain construction

[0051] 1. Cloning of Arabidopsis glycosyltransferase gene UGT84A2

[0052] The glycosyltransferase gene UGT84A2 involved in the present invention is cloned from Arabidopsis thaliana through RT-PCR amplification technology. The primers used in the amplification are: UGT84A2-a: 5'-TCGGATCCATGGAGCTAGAAT-3' (SEQ ID NO: 3); UGT84A2-b: 5'-CAGTCGACTTAAAAGCTTT-3' (SEQ ID NO: 4).

[0053] The RT-PCR amplification program is: 94°C (pre-denaturation), 5min; 94°C (denaturation), 10s; 55°C (annealing), 15s; 72°C (extension), 1.5min; 35cycle; 72°C (final extension) , 10min. Recover and purify the amplified product. The amplified target gene UGT84A2 was ligated with EcoRV single digested intermediate vector pBluescript SK to obtain vector pB84A2, and the cloned gene was sequenced.

[0054] 2. Sequence information and characteristics of UGT84A2

[0055] After sequencing...

Embodiment 3

[0060] Example 3 Phenotype analysis of Arabidopsis UGT84A2 high-expressing strains

[0061] 1. Late flowering phenotype analysis of Arabidopsis UGT84A2 high-expressing lines

[0062] At the reproductive growth stage, the flowering time of wild-type and over-expressing bodies was counted (with bolting 0.5 cm as the standard), and it was found that the high expression of UGT84A2 caused the phenotype that the flowering period was delayed by 4-7 days (see image 3 ). Counting the number of rosette leaves during bolting of the above two lines, it was found that the number of rosette leaves during bolting in WT was 12.29±0.49, mutant brt1-1 was 11.67±0.50, mutant 84a2 3-13 was 11.84±0.78, and overexpression body 84A2OE 2 -2 is 13.67±0.58, and the overexpression body 84A2OE 8-4 is 15.33±0.71 (see Figure 4 ), the number of leaves of the overexpression body is more than that of the wild type.

[0063] 2. Leaf phenotype analysis of Arabidopsis UGT84A2 highly expressing lines

[0064] In the v...

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Abstract

The invention discloses application of glycosyl transferase UGT84A2 of Arabidopsis in regulation of flowering time of plants. According to overexpression of glycosyl transferase UGT84A2 of Arabidopsis, plant growth and development can be regulated, and agronomic traits such as flowering delay, increase of rosette leaves during flowering and / or lengthened petiole at a vegetative growth phase are produced. The flowering time is an important agronomic trait in crop production. According to the application in the invention, significant economy benefits are brought to agricultural production.

Description

Technical field [0001] The invention belongs to the technical field of plant growth and development regulation and crop genetic improvement, and specifically relates to an application of a glycosyltransferase to regulate plant growth and development, and in particular to an application of an Arabidopsis glycosyltransferase UGT84A2 in regulating the flowering time of plants. Background technique [0002] Flower development is a key link for higher plants to transform from vegetative growth to reproductive growth and realize generational alternation. Plant flowering is a comprehensive development process, including flowering induction, signal transmission, attribute determination, organogenesis, and involves the conversion of different developmental modes. The flowering induction process of higher plants is determined by its own genetic factors and external environmental factors, and is the result of the sequential expression of flowering genes in time and space. Plants receive th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H5/00C12N9/10C12N15/54
CPCC12N9/1048C12N15/827
Inventor 张桂芝
Owner LINYI UNIVERSITY
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