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Method for rapidly identifying and detecting manuka honey

A manuka honey, identification and detection technology, which is applied to measuring devices, instruments, scientific instruments, etc., can solve the problems of shoddy charging and adulteration, and achieve improved accuracy, sensitivity and high accuracy and high sensitivity. Effect

Inactive Publication Date: 2017-12-08
ANIMAL AND PLANT & FOOD DETECTION CENTER JIANGSU ENTRY EXIT INSPECTION AND QUARANTINE BUREAU +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the standard, only if it meets the color ≥ 62mmpfund, conductivity: 347-867μS / cm, taste: mineral flavor, slightly bitter, smell: wet earth, smell similar to heather plant, smell aromatic, pollen and energy of Manuka plant The honey with dihydroxyacetone (DHA) and methylglyoxal (MGO) indicators can only be marked with the word "Manuka" on the product label. However, these indicators cannot fundamentally rule out shoddy, adulterated and false The problem

Method used

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  • Method for rapidly identifying and detecting manuka honey
  • Method for rapidly identifying and detecting manuka honey
  • Method for rapidly identifying and detecting manuka honey

Examples

Experimental program
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Effect test

Embodiment 1

[0051] The investigation and establishment of embodiment 1 liquid chromatography, mass spectrometry method

[0052] Standards and reagents used in this embodiment are: 3,5-dimethoxybenzoic acid methyl ester-4-diglucoside purity is greater than 99%; Experimental acetonitrile (chromatographically pure) is purchased from German Merck company; Formic acid (chromatographically pure) Pure) from TEDIA, USA.

[0053] Accurately weigh an appropriate amount of standard substance, make a 1.0mg / mL standard stock solution with water, and store it in the refrigerator at 4°C. Dilute with acetonitrile water (1:9v / v) to a working solution with a concentration of 100μg / ml.

[0054] 1. Establishment of liquid chromatography conditions

[0055] A small amount of formic acid was added during liquid phase analysis.

[0056] The high performance liquid chromatography conditions are as follows:

[0057] Column: Dikma Diamonsil Plus C 18 150*4.6mm chromatographic column;

[0058] Mobile phase: A...

Embodiment 2

[0075] Determination of 3,5-dimethoxybenzoic acid methyl ester-4-diglucoside content in the honey of embodiment 2

[0076] 1. Content analysis of 3,5-dimethoxybenzoic acid methyl ester-4-diglucoside in manuka honey

[0077] Accurately weigh 1.0 g of the melted sample (accurate to 0.01 g) into a quantitative bottle, dilute to 10.0 mL with water, vortex until dissolved, take an appropriate amount of supernatant to filter the membrane, and prepare for sample analysis.

Embodiment 3

[0082] Example 3 Methodological investigation of methyl 3,5-dimethoxybenzoate-4-diglucoside as a characteristic marker of manuka honey

[0083] The standard stock solution was diluted with acetonitrile water (1:9v / v) into the standard working solution, and the contents were 1.0μg / mL, 5.0μg / mL, 10.0μg / mL, 20.0μg / mL, 50.0μg / mL and 100.0μg / mL standard solution, determined according to the following conditions, and quantified by the external standard method.

[0084] (1) High performance liquid chromatography separation conditions are as follows,

[0085] Column: Dikma Diamonsil Plus C 18 150*4.6mm chromatographic column;

[0086] Mobile phase: A is 0.1% (v / v) formic acid aqueous solution, B is acetonitrile;

[0087] Flow rate: 0.8mL / min;

[0088] Gradient elution program: 0-2min 70%A, 2-12min 70-10%A, 12-15min 10%A, 15-16min10-90%A, 16-18min 70%A;

[0089] Column temperature: 25°C;

[0090] Injection volume: 25 μL.

[0091] (2) The mass spectrometry detection selects a he...

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Abstract

The invention belongs to the field of food detection, and particularly relates to a method for rapidly identifying and detecting manuka honey. The detection method is characterized in that efficient liquid chromatography-quadrupole electrostatic field orbitrap high-resolution mass spectrum is treated as the detection manner, and 3, 5-methyl dimethoxybenzoate-4-diglucoside is treated as a characteristic detection mark, and, on that basis, the manuka honey can be rapidly and accurately identified. The method is high in integrity, high in specificity, high in controllability, and high in accuracy. With the adoption of the method, the technical support is supplied to the identification analyzing of the manuka honey; the legitimate rights of consumers are protected; the method is of important significance on bee product market standardizing and the promoting of healthy development of bee product business.

Description

technical field [0001] The invention belongs to the field of food detection, in particular to a rapid identification and detection method of Manuka honey. Background technique [0002] Manuka honey is a precious honey species unique to New Zealand. It is brewed by bees from the nectar of Manuka (Leptospermum scoparium), a shrub unique to New Zealand. What sets it apart from other honeys is the powerful and unique antibacterial activity of Manuka honey. Honey generally has antibacterial activity, which is caused by the high permeability of honey itself, strong acidity and generally containing peroxides. The antibacterial activity of manuka honey is not dependent on peroxide, so it is called non-peroxide antibacterial activity (NPA). At present, New Zealand's manuka honey exports have increased by about 10% every year for the past 10 years. In 2013, the value of honey exported from New Zealand was 128 million New Zealand dollars. However, according to statistics from a New ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72
Inventor 吴斌张睿丁涛邓晓军张峰刘芸费晓庆张晓燕柳菡沈伟健陈磊郭德华伊雄海何宇平
Owner ANIMAL AND PLANT & FOOD DETECTION CENTER JIANGSU ENTRY EXIT INSPECTION AND QUARANTINE BUREAU
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