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Functional protein peptide product

A technology of functional protein and protein peptide, which is applied in the field of biological protein, can solve the problems of product quality to be improved, long preparation time, light yellow color, etc., and achieve the goal of reducing time cost and labor cost, good control effect and low production cost Effect

Inactive Publication Date: 2017-12-12
ZHEJIANG FENGYU MARINE BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above antioxidant peptide products take a long time to prepare, have a fishy smell and are light yellow in color, and the product quality needs to be improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A functional protein peptide product, the amino acid sequence is SCASRCKSRCRARRCRARCGYFGKRCICKCFRC.

[0021] A method for preparing a functional protein peptide product includes the following steps:

[0022] 1) Chymolysis: Add chymotrypsin to crucian carp minced meat, the amount of chymotrypsin added is 0.1~0.3%, the enzymolysis temperature is 45~55℃, the pH is 7.5~8.5, the enzymolysis time It is 15~25min. Microwave-assisted enzymolysis, microwave power is 100~120W, frequency is 20~30kHZ. After enzymolysis, the enzyme is inactivated in a boiling water bath for 2 to 5 minutes. Microwave-assisted enzymolysis can greatly shorten the time of protease enzymolysis, reduce time and labor costs, and reduce production costs. Chymotrypsin enzymolyzes the viscous fish meat into a solid-liquid mixture with a smaller viscosity and molecular weight, exposing more restriction sites to prepare for the next enzymolysis;

[0023] 2) Papain enzymolysis: add papain to the enzymolysis solution...

Embodiment 2

[0031] A functional protein peptide product, the amino acid sequence is SCASRCKSRCRARRCRARCGYFGKRCICKCFRC.

[0032] A method for preparing a functional protein peptide product includes the following steps:

[0033] 1) Chymolysis: Add chymotrypsin to crucian carp minced meat, the amount of chymotrypsin added is 0.2%, the enzymolysis temperature is 50℃, the pH is 8.0, and the enzymolysis time is 20min. Microwave-assisted enzymolysis, microwave power is 110W, frequency is 25kHZ. After enzymolysis, the enzyme is inactivated in a boiling water bath for 4 minutes. Microwave-assisted enzymolysis can greatly shorten the time of protease enzymolysis, reduce time and labor costs, and reduce production costs. Chymotrypsin enzymolyzes the viscous fish meat into a solid-liquid mixture with a smaller viscosity and molecular weight, exposing more restriction sites to prepare for the next enzymolysis;

[0034] 2) Papain enzymolysis: add papain to the enzymolysis solution, the amount of papain add...

Embodiment 3

[0042] A functional protein peptide product, the amino acid sequence is SCASRCKSRCRARRCRARCGYFGKRCICKCFRC.

[0043] A method for preparing a functional protein peptide product includes the following steps:

[0044] 1) Chymolysis: Add chymotrypsin to crucian carp minced meat. The addition amount of chymotrypsin is 0.3%, the enzymolysis temperature is 50℃, the pH is 8.0, and the enzymolysis time is 16min. Microwave-assisted enzymatic hydrolysis, microwave power is 110W, frequency is 25kHZ. After enzymolysis, the enzyme is inactivated in a boiling water bath for 4 minutes. Microwave-assisted enzymolysis can greatly shorten the time of protease enzymolysis, reduce time and labor costs, and reduce production costs. Chymotrypsin digests the viscous fish meat chyme into a solid-liquid mixture with a smaller viscosity and molecular weight, exposing more enzyme cutting sites to prepare for the next enzymolysis;

[0045] 2) Papain enzymolysis: add papain to the enzymolysis solution, the amo...

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Abstract

The invention discloses a functional protein peptide product. An amino acid sequence is SCASRCKSRCRARRCRARCGYFGKRCICKCFRC. A preparation method comprises the steps of chymotrypsin enzymolysis, papain enzymolysis, isoelectric precipitation, decoloration and fishy smell removal and purification. The functional protein peptide product has the advantages that a step-by-step enzymolysis method is adopted for enzymolysis, the enzymolysis efficiency of protease is high, the cost is reduced, enzymolysis is performed by sequentially adopting chymotrypsin and papain, and a large amount of micromolecular protein and polypeptide are obtained. The obtained functional protein peptide has the repairing effect on pancreatic beta cell, insulin structure gene transcription is promoted to form messenger ribonucleic acid (mRNA) of preproinsulin, and insulin is synthesized in a ribosome of endoplasmic reticulum. Therefore, the functional protein peptide product has the better preventing and treating effect on diabetes.

Description

Technical field [0001] The invention relates to the technical field of biological proteins, in particular to a method for preparing functional protein peptide products. Background technique [0002] With the development of society and economy, people's health awareness has become stronger and stronger, and they have paid more and more attention to the quality and nutrition of food. Biologically active peptides are multifunctional compounds derived from proteins. They play an important regulatory role in life activities. They are easy to digest and absorb. They can promote immunity, hormone regulation, antibacterial, antiviral, antioxidant, blood pressure, blood lipid, and blood sugar. It has high edible safety, and is currently the most popular research topic in the international food industry and a functional factor with great development prospects. At present, the research of functional peptides has been listed in the high-tech fields supported by the state. [0003] Crucian ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/46C07K1/36C07K1/34C07K1/30C07K1/20C07K1/18C12P21/06
CPCC07K14/461C12P21/06
Inventor 周龙锡张银兴
Owner ZHEJIANG FENGYU MARINE BIOLOGICAL PROD