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Production method of low-molecular weight tremella polysaccharide

A tremella polysaccharide and low molecular weight technology, which is applied to the production method of low molecular weight tremella polysaccharide and its products, can solve the problems of many impurities and low yield, and achieve the effects of improving efficiency and simplifying operations

Active Publication Date: 2017-12-12
BLOOMAGE BIOTECHNOLOGY CORP LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to reduce the molecular weight of tremella polysaccharide and solve the problems of high impurities and low yield, the invention provides a production method of low molecular weight tremella polysaccharide and low molecular weight tremella polysaccharide with high purity

Method used

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  • Production method of low-molecular weight tremella polysaccharide

Examples

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Embodiment 1

[0028] Example 1 Preparation of Low Molecular Weight Tremella Polysaccharide

[0029] (1) Alcohol precipitation: Add 4% (w / v) sodium chloride into 4L tremella spore fermentation broth, fully dissolve, and precipitate with 95% ethanol that is 2.5 times the volume of the fermentation broth to obtain the crude product;

[0030] (2) Acid hydrolysis: dissolve the above crude product in water, adjust to a viscosity of 12000 mPa.s, add hydrochloric acid to a concentration of 0.2 mol / L, and acidify at 70°C for 12 hours;

[0031] (3) Decolorization: Use 40% NaOH to adjust the pH value of the acid solution in step (2) to 5.5, add 1% (w / v) 200-mesh activated carbon, absorb at 55°C for 2 hours, and filter with a plate frame to obtain the filtrate ;

[0032] (4) Protein removal: adjust the pH of the filtrate to 11.0, filter it with a plate frame after 1 hour, and fine filter it through a 0.22 μm polyethersulfone filter element to obtain a fine filtrate;

[0033] (5) Refining: Precipitate...

Embodiment 2

[0035] The preparation of embodiment 2 low molecular weight white fungus polysaccharides

[0036] (1) Alcohol precipitation: Add 5% (w / v) sodium chloride into 4L tremella spore fermentation broth, fully dissolve, and precipitate with 93% ethanol that is three times the volume of the fermentation broth to obtain the crude product;

[0037] (2) Acid hydrolysis: dissolve the above crude product in water, adjust the viscosity to 10,000 mPa.s, add hydrochloric acid to a concentration of 0.2 mol / L, and acidify at 75°C for 10 h;

[0038] (3) Decolorization: adjust the pH value of the acidolysis solution in step (2) to 5.8 with 40% NaOH, add 1% (w / v) 200 mesh activated carbon, absorb at 60°C for 1.5 h, and filter with a plate frame to obtain filtrate;

[0039] (4) Protein removal: adjust the pH of the filtrate to 11.0, filter it with a plate frame after 2 hours, and fine filter it through a 0.22 μm polyethersulfone filter element to obtain a fine filtrate;

[0040] (5) Precipitate t...

Embodiment 3

[0042] The preparation of embodiment 3 low molecular weight white fungus polysaccharides

[0043] (1) Alcohol precipitation: Add 3% (w / v) sodium chloride into 4L tremella spore fermentation broth, fully dissolve, and precipitate with 90% ethanol 3 times the volume of the fermentation broth to obtain the crude product;

[0044] (2) Dilute acid acid hydrolysis: dissolve the above crude product in water, adjust the viscosity to 10000 mPa.s, add hydrochloric acid to a concentration of 0.2mol / L, and acid hydrolyze at 80°C for 8 hours;

[0045] (3) Decolorization: adjust the pH value of the acidolysis solution in step (2) to 6.0 with 40% NaOH, add 2% (w / v) 200 mesh activated carbon, absorb at 65°C for 1 h, and filter with a plate frame to obtain filtrate;

[0046] (4) Protein removal: adjust the pH of the filtrate to 11.0, filter it with a plate frame after 1 hour, and perform fine filtration through a polyethersulfone filter element with a pore size of 0.22 μm to obtain a fine fil...

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Abstract

The invention provides a production method of low-molecular weight tremella polysaccharide. The production method comprises the following steps of using alcohol to settle tremella polysaccharide fermenting liquid produced by a fermenting method, acidifying, discoloring, removing protein, refining, drying, and the like, so as to obtain a low-molecular weight tremella polysaccharide product. The low-molecular weight tremella polysaccharide has the advantages that the wall breaking and the hydrolysis are combined by acidifying, so that the operation is simplified, and the yield rate can reach 26g / L; the relative molecular weight of the prepared tremella polysaccharide is lower than 100kDa, the content is greater than 80%, the content of protein is smaller than 0.5%, the yield rate is high, the content is high, the impurity is less, and the low-molecular weight tremella polysaccharide can be widely applied to the fields of medicines and daily chemicals.

Description

technical field [0001] The invention relates to a production method of tremella polysaccharide, in particular to a production method of low molecular weight tremella polysaccharide and its products. Background technique [0002] Tremella polysaccharide is the main active substance in tremella. It has no sweet taste and is easily soluble in hot water. The aqueous solution has optical activity and no mutarotation. Most tremella polysaccharides are mannose linked by α-(1→3) bonds as the main chain, mainly composed of xylose, glucose, fucose, mannose and glucuronic acid. [0003] Tremella polysaccharide has excellent moisturizing and water-locking ability. It is used in skin care cosmetics. It has the functions of repairing the epidermis and increasing the water content of the epidermis, increasing the water content of the stratum corneum, increasing the integral value of skin conductivity, water retention capacity, reducing the water release constant, and improving The skin's ...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003C08B37/006
Inventor 张天萌郭文逸孙劭靖郭学平
Owner BLOOMAGE BIOTECHNOLOGY CORP LTD