Immunoglobulin A detection kit and detection method thereof
An immunoglobulin and detection kit technology, which is applied in biological testing, measuring devices, material testing products, etc., can solve the problems of inaccurate detection results of reagents, inability to meet the requirements for use, affecting the detection effect, etc., and achieve good stability of the reagents. , wide versatility, accurate detection results
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Embodiment 1
[0040] In order to enable those skilled in the art to better understand the technical solutions in the application, the present invention will be further described below in conjunction with the embodiments. Obviously, the described embodiments are only some of the embodiments of the application, not all of them. Based on the embodiments in this application, all other embodiments obtained by persons of ordinary skill in the art without creative efforts shall fall within the scope of protection of this application. Embodiment 1 Immunoglobulin A detection kit
[0041] Reagent R1:
[0042] TRIS buffer
20mmol / L
Sodium chloride
1g / L
Sodium azide
0.5g / L
polyethylene glycol 6000
1g / L
Ethylphenyl polyethylene glycol
1g / L
[0043] Reagent R2:
[0044] TRIS buffer
20mmol / L
Sodium chloride
1g / L
Sodium azide
0.5g / L
Goat anti-human immunoglobulin A antibody
10mg / L
Ethylphenyl ...
Embodiment 2
[0047] Embodiment 2 Immunoglobulin A detection kit
[0048] Reagent R1:
[0049] Phosphate buffer
40mmol / L
potassium chloride
7g / L
phenol
0.7g / L
polyethylene glycol 6000
30g / L
Ethylphenyl polyethylene glycol
50g / L
[0050] Reagent R2:
[0051] Phosphate buffer
40mmol / L
potassium chloride
5g / L
phenol
0.7g / L
Goat anti-human immunoglobulin A antibody
50mg / L
Ethylphenyl polyethylene glycol
50g / L
magnesium sulfate
15.5g / L
calcium acetate
23g / L
[0052] Calibrator:
[0053] Immunoglobulin A antigen standard diluent (50mmol / L glycine buffer, 10g / L sodium chloride, 0.5g / L sodium azide, 20g / L dextran, 25g / L trehalose, 30g / L sucrose , 20g / L bovine serum albumin) was dissolved, tested with a commercially available control reagent and adjusted to 100mg / L, and stored in -20°C. Take it out before use, and dilute it with standard diluent to obt...
Embodiment 3
[0054] Embodiment 3 Immunoglobulin A detection kit
[0055] Reagent R1:
[0056] acetate buffer
80mmol / L
potassium chloride
20g / L
Sodium azide
0.8g / L
polyethylene glycol 6000
45g / L
Ethylphenyl polyethylene glycol
5g / L
[0057] Reagent R2:
[0058] acetate buffer
80mmol / L
potassium chloride
20g / L
Sodium azide
1g / L
Goat anti-human immunoglobulin A antibody
300mg / L
Ethylphenyl polyethylene glycol
5g / L
magnesium sulfate
0.05g / L
calcium acetate
0.04g / L
[0059] Calibrator:
[0060] Immunoglobulin A antigen standard diluent (70mmol / L TRIS buffer, 25g / L sodium chloride, 1g / L sodium azide, 55g / L dextran, 50g / L trehalose, 60g / L sucrose, 75g / L bovine serum albumin) was dissolved, detected and adjusted to 200mg / L with a commercially available control reagent, subpackaged and stored at -20°C. Take it out before use, and dilute it with standa...
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