Synthesis method of amoxicillin artificial antigen

A technology of amoxicillin and artificial antigen, which is applied in the field of biochemical industry, can solve the problems of low product synthesis rate, many by-products, reducing antigen immunogenicity, etc., and achieve the effects of enhancing immune effect, increasing molecular weight, and enhancing immunogenicity Effect

Inactive Publication Date: 2017-12-15
樊之雄
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Problems solved by technology

[0005] Technical problem to be solved: the existing method for synthesizing amoxicillin antigen with carbodiimide has a low product synthesis rate and many by-products, and amoxicillin is relatively stable only under acidic conditions, and is easily hydrolyzed after entering the body. Thereby reducing the immunogenicity of the antigen, so it is necessary to further improve the synthesis yield and immunogenicity of the antigen

Method used

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  • Synthesis method of amoxicillin artificial antigen
  • Synthesis method of amoxicillin artificial antigen
  • Synthesis method of amoxicillin artificial antigen

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Embodiment 1

[0029] A kind of synthetic method of amoxicillin artificial antigen, comprises the steps:

[0030] (1) Synthesis of haptens

[0031] Amoxicillin and sulbactam were coupled by the carbodiimide method at a molar ratio of 1:1.5. First, the sulbactam was dissolved with 2-morpholineethanesulfonic acid buffer, and then the sulbactam was mixed with carbon diimide The imine was added to the carbodiimide solution at a molar ratio of 1:4, and after reacting for 2 hours under magnetic stirring, the reaction solution was freeze-dried to obtain a sulbactam-coupled amoxicillin hapten solid.

[0032] (2) Synthesis of complete antigen

[0033] Dissolve 2 mg of the hapten obtained in step (1) with 1 mL of N-N dimethylformamide, add 20 μL of tri-n-butylamine, react at 4°C for 20 min, then add 35 μL of isobutyl chloroformate to continue the reaction for 2 h, and dissolve it Add dropwise at a molar ratio of 50:1 to bovine serum albumin BSA dissolved in 0.01M pH7.2 hydroxyethylpiperazine ethanes...

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Abstract

The invention discloses a synthesis method of an amoxicillin artificial antigen, and belongs to the technical field of biochemical industry. The synthesis method comprises the following steps: coupling an amoxicillin parent nuclear structural analogue sulbactam and an amoxicillin hapten to obtain a novel amoxicillin hapten, then coupling the hapten and bovine serum albumin (BSA) by an isobutyl chloroformate method, and dialyzing to obtain an amoxicillin complete antigen. Through gel electrophoresis analysis on the complete antigen and a BSA protein band, a result shows that the amoxicillin is successfully coupled to the BSA, and an antibody obtained through animal immunization confirms the successful synthesis of the artificial antigen. By the synthesis method, the molecular weight of the amoxicillin is increased, the immunogenicity of the amoxicillin is further enhanced, the immunizing effect is enhanced, and an important basis is provided for preparation of a high-sensitivity amoxicillin antibody.

Description

technical field [0001] The invention discloses a method for synthesizing an artificial antigen of amoxicillin, which belongs to the technical field of biochemical engineering. Background technique [0002] Amoxicillin (AMX), also known as amoxycillin or amoxycillin, is the most commonly used semi-synthetic penicillin broad-spectrum β-lactam antibiotic, and is one of the most widely used oral semi-synthetic penicillins. The spectrum and antibacterial activity are basically the same as ampicillin, but its acid resistance is stronger than that of ampicillin, and its bactericidal effect is stronger and faster than that of the latter. The half-life is about 61.3 minutes, and it is more stable under acidic conditions. Amoxicillin has a strong bactericidal effect and a strong ability to penetrate the cell wall. After oral administration, the lactam group in the drug molecule is immediately hydrolyzed to form a peptide bond, which quickly binds to the transpeptidase in the bacteria ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/765C07K1/113C07K16/44
CPCC07K14/765C07K16/44C07K19/00
Inventor 樊之雄
Owner 樊之雄
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