Application of DGM1 protein in improving generation capacity of plant root hairs

A technology that generates 1. DGM1, applied in applications, plant peptides, plant products, etc., can solve problems such as unclear molecular mechanisms, achieve small risks, and improve the effect of root hair length and density

Active Publication Date: 2017-12-15
TSINGHUA UNIV
View PDF3 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the molecular mechanisms regu...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of DGM1 protein in improving generation capacity of plant root hairs
  • Application of DGM1 protein in improving generation capacity of plant root hairs
  • Application of DGM1 protein in improving generation capacity of plant root hairs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1, the acquisition of AtDGM1 gene and its functional verification

Embodiment approach

[0051] In order to identify important genes involved in the regulation of plant root hair development, the inventors of the present invention conducted screening of Arabidopsis mutants with abnormal root hairs. A mutant hps5 with increased root hair formation under normal conditions was obtained by screening the mutant library of Arabidopsis thaliana seeds. Gene cloning revealed that HPS5 encodes the ethylene receptor ERS1. Through the comparative analysis of the transcriptomes of hps5 and the ethylene-insensitive mutant ein3, etc., the inventors identified a series of target genes involved in root hair development downstream of ethylene, and proved that EIN3 can directly bind to the promoters of these root hair-related genes Above all, the expression levels of these target genes were increased, thereby increasing the formation of root hairs. Most of these genes encode cell wall proteins, cell wall modifying enzymes, proton pumps, jasmonic acid signaling and synthesis-related...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an application of DGM1 protein in improving generation capacity of plant root hairs and provides an application of DGM1 protein or an encoding gene thereof in improving generation capacity of plant root hairs; and the DGM1 protein can be any protein of sequence number 2-6 in a sequence list. In the application disclosed by the invention, AtDGM1 is overexpressed in plants and is capable of significantly increasing length and density of the plant root hairs, without changing other growth and developmental traits of plants. As coming from plants diretly, an AtDGM1 gene has a relatively low risk in biosafety. In addition, as the AtDGM1 gene has homologous genes in other important crops such as rice, soybean, rape and tomato, more alternatives are provided during construction of transgenic plants. The application disclosed by the invention provides genetic resources for cultivating new crop varieties which efficiently absorb soil nutrients by utilizing genetic engineering means, and can be used for cultivating the new crop varieties which efficiently absorb soil nutrients.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the application of DGM1 protein in improving plant root hair generation ability. Background technique [0002] The surface area of ​​root hairs accounts for about 70% of the total surface area of ​​plant roots. It is the most active tissue for roots to absorb water and nutrients, and is an important part of plant roots' perception of external signals. Root hairs are unicellular, tubular projections formed by the outgrowth of specific epidermal cells in the mature zone of the root. Root hairs are the direct contact part between the plant root and the soil. The increase in the number and length of the root hair can increase the contact area between the plant root epidermal cells and the soil, and help to improve the stability of the root in the soil, the interaction between the root and microorganisms, and the uptake of soil nutrients. Root hairs can also affect the environment around ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00
CPCC07K14/415C12N15/8261
Inventor 刘栋宋丽
Owner TSINGHUA UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap