Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting doping of other collagen-containing substances in edible bird's nest

A technology containing collagen and bird's nest, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of unsatisfactory reproducibility and easy blockage of pipelines, achieve good reproducibility, ensure accuracy, reliability and accuracy high effect

Active Publication Date:
View PDF5 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The amino acid analyzer adopts cation exchange chromatographic separation and post-column ninhydrin derivatization spectrophotometric detection technology. The detection process of this method takes up to 1 hour, and the ninhydrin precipitation is easy to block the pipeline during the operation of the instrument, and the reproducibility is not good. ideal

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting doping of other collagen-containing substances in edible bird's nest
  • Method for detecting doping of other collagen-containing substances in edible bird's nest
  • Method for detecting doping of other collagen-containing substances in edible bird's nest

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0028] Use the method of the present invention to detect the content of 22 kinds of amino acids in 10 batches of bird's nests and judge whether there are other collagen-containing substances

[0029] 1. Instruments and reagents

[0030] Instruments: Agilent 1260infinity FLD detector with automatic sampler (1329B); Milli Q purification system; Nitrogen blowing instrument (Hangzhou Xuezhong Charcoal Company); 1 / 100,000 balance (Mettler Toledo Company);

[0031] Reagents: Borate buffer, OPA, FMOC, 17 kinds of amino acid mixed standards, asparagine, glutamine, tryptophan, hydroxyproline, sarcosine standard reagents are all Agilent; hydrochloric acid, dihydrogen phosphate Sodium and phenol are analytically pure; Lithium hydroxide is a product of Sigma Company, superior grade; Methanol and acetonitrile are products of Merck, chromatographically pure; experimental water is ultrapure water from Milli Q purification system. The sources and types of 10 batches of bird’s nest supplement...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for detecting doping of other collagen-containing substances in edible bird's nest. The method comprises the following steps: 1) pre-treatment of a sample of edible bird's nest: dividing the sample of the edible bird's nest into two parts, wherein one part employs acid for hydrolysis treatment and fixes the volume to be taken as an acid hydrolysis sample, the other part employs basic for hydrolysis treatment and fixes the volume to be taken as a basic hydrolysis sample, mixing the acid hydrolysis sample and the basic hydrolysis sample, fixing the volume, and taking a mixture as the pre-treatment sample; 2) pre-column derivatization treatment: respectively performing pre-column derivatization on primary amino acid and secondary amino acid of the pre-treated sample by OPA and FMOC to obtain a to-be-measured sample; and 3) respectively performing high performance liquid chromatography detection and analysis on the to-be-measured sample and a standard sample, by comparing the detection spectrums, determining whether the to-be-measured sample contains hydroxyproline and / or sarcosine or not, and whether the standard sample contains the standard object of hydroxyproline and / or sarcosine or not. The method is helpful for determination of quality of the edible bird's nest, and has the advantages of rapid analysis process and sensitive detection.

Description

technical field [0001] The invention belongs to the technical field of bird's nest identification and analysis, in particular to a method for detecting whether the bird's nest is doped with other collagen-containing substances. Background technique [0002] Bird's nest (Cubilose) is a nest built by swiftlets of the Swift family Swiftlet family and a variety of swiftlets belonging to the same species with saliva and a small amount of feathers mixed and bonded. According to modern research, bird's nest is rich in active sialic acid glycoprotein, minerals, carbohydrates, vitamins and amino acids and other natural nutrients, and has the effects of nourishing the middle and Qi, nourishing yin and moistening dryness, etc. It is a valuable nourishing food one. [0003] People's demand for bird's nest and its related products is constantly increasing. At present, there is no uniform product standard in the bird's nest industry in the market, and the quality is uneven. Some unscrupu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 上官国莲梁雪琪黎小鹏温玉辉
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com