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GFP-labeled Fusarium proliferatum and applications thereof

A green fluorescent layer, fusarium technology, applied in the direction of application, fungi, gardening, etc.

Inactive Publication Date: 2017-12-22
CHINA NAT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, in the prior art, there is no application of Fusarium laminarum labeled with green fluorescent protein (green fluorescent protein, GFP) for ear rot of rice.

Method used

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  • GFP-labeled Fusarium proliferatum and applications thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1: green fluorescent layer goes out fusarium ( Fusarium proliferatum) Isolation and identification of FP-GFP

[0013] Susceptible rice ears were taken from areas A-D of Fuyang Experimental Base of China Rice Research Institute in Hangzhou City, Zhejiang Province and the experimental field of Plant Protection Station in Jiaxing City, Zhejiang Province, and stored in vacuum at low temperature. The rice varieties for sampling mainly include "Xiushui 09" (japonica rice), "Yongyou" and "Chunyou" series (indica-japonica hybrid rice), "Guodao No. 6" (super hybrid rice) and other varieties (combinations).

[0014] Potato dextrose agar medium (PDA): distilled water 1000 mL, potato 200 g, agar powder 20 g, glucose 20 g, high temperature and high pressure sterilization (121 ° C, 2.4 × 10 5 Pa, 20 min) before use.

[0015] Water agar medium (WA): 1 000 mL of distilled water, 20 g of agar powder, sterilized at high temperature and high pressure (121°C, 2.4×10 5 Pa, 2...

Embodiment 2

[0024] Embodiment 2: green fluorescent layer goes out fusarium ( Fusarium proliferatum) Applications of FP-GFP

[0025] Through field and indoor inoculation experiments, the green fluorescent pathogenic bacteria can be used to conduct research on disease tracking, disease transmission speed, and disease severity prediction.

[0026] Through field and indoor inoculation experiments, pathogenic bacteria are isolated by sampling, and then the number of green fluorescent strains is observed under a microscope to obtain a lot of useful data. For example, disease tracking can be obtained by sampling different parts of the plant to see if there are green fluorescent bacteria in the isolated pathogenic bacteria. Achieve the goal; the disease transmission speed can be obtained by sampling the areas at different distances from the inoculation area, and counting the presence or absence of green fluorescent bacteria isolated in the area samples at different distances; the severity of the ...

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Abstract

The invention relates to GFP-labeled Fusarium proliferatum and applications thereof, belongs to the field of biotechnology, and provides a GFP-labeled Fusarium proliferatum strain FP-GFP and applications thereof. According to the present invention, through field experiments, indoor experiments and other inoculation experiments of the GFP-labeled Fusarium proliferatum strain FP-GFP, disease tracking can be performed with the GFP-labeled pathogenic bacteria so as to study the disease propagation speed, the disease severity prediction and other fields.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a green fluorescent layered Fusarium fungus and its application. Background technique [0002] Rice spikelet rot disease (RSRD) is a late-stage panicle disease of rice, which causes rot, discoloration, and deformity of mature rice, resulting in reduced or false seed setting rate and seriously affects yield. Also due to the pigment and toxin produced by pathogenic bacteria, the appearance of rice is changed and the quality of rice is reduced. Ear rot, also known as ear browning, glume blight, and grain blight, is a fungal disease. After rice infection, the grains will rot, and the toxins produced by the pathogens will also cause harm to the health of humans and animals. The main infection source of rice ear rot is very complex, generally mixed infection by various fungi. The types of fungi isolated in China generally include Curvularia, Alternaria, Fusarium and Phoma, et...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01G13/00C12R1/77
CPCA01G13/00C12N1/145C12R2001/77
Inventor 孙磊刘联盟王玲黄世文
Owner CHINA NAT RICE RES INST
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